Detection of plasmid-borne mcr genes conferring colistin resistance in MDR and XDR Gram-negative bacterial isolates from an Egyptian hospital
Background: Infections with multi-drug resistant (MDR) Gram-negative bacteria represent a serious public health risk, especially with the emergence of colistin resistance. Colistin resistance is mainly mediated by chromosomal mutations; however, there are reports of transferable plasmid-mediated col...
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Zagazig University, Faculty of Medicine
2025-08-01
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| Series: | Microbes and Infectious Diseases |
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| Online Access: | https://mid.journals.ekb.eg/article_422773_5ee7e792e3f03b755d38724453bf162d.pdf |
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| author | Doaa Yousef Ahmed Soliman Fatma Sonbol Amal Abo Kamer Hazem Ramadan Mahmoud Farghali |
| author_facet | Doaa Yousef Ahmed Soliman Fatma Sonbol Amal Abo Kamer Hazem Ramadan Mahmoud Farghali |
| author_sort | Doaa Yousef |
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| description | Background: Infections with multi-drug resistant (MDR) Gram-negative bacteria represent a serious public health risk, especially with the emergence of colistin resistance. Colistin resistance is mainly mediated by chromosomal mutations; however, there are reports of transferable plasmid-mediated colistin resistance genes, namely mcr, which have been universally documented. Thus, our study aimed to examine the incidence of mobile colistin resistance genes (mcr-1 and mcr-2) among MDR and extensively drug-resistant (XDR) Gram-negative bacteria. Methods Two hundred and forty-two Gram-negative clinical bacterial isolates were obtained in our study. Using standard microbiological methods, the bacteria were isolated and identified. Colistin resistance was phenotypically detected utilizing the broth microdilution technique. The colistin-resistant isolates were examined for their antimicrobial susceptibility profile using the Kirby-Bauer disk diffusion method. We used PCR to identify the mcr-1 and mcr-2 genes. Results Our data revealed that up to 18 (7.8%) isolates were colistin-resistant, including 11 Klebsiella spp. isolates, six Pseudomonas spp. isolates and only one Escherichia coli isolate. The PCR results revealed that mcr-1 was found in two isolates (11.1%), including one isolate of Klebsiella spp. (colistin MIC=32 µg/ml) and one isolate of Escherichia coli (colistin MIC=4 µg/ml). None of the colistin-resistant isolates carried mcr-2. Conclusions Based on our data, a relatively low incidence of colistin resistance was observed among clinical isolates. However, the detection of mcr-1 in two isolates of different species is concerning because of the possibility of spreading to susceptible strains. Public Health authorities should implement colistin resistance monitoring programs and infection control strategies in healthcare settings. |
| format | Article |
| id | doaj-art-ce7fa8060b8343e886da4412ea4f1d12 |
| institution | Kabale University |
| issn | 2682-4132 2682-4140 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Zagazig University, Faculty of Medicine |
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| series | Microbes and Infectious Diseases |
| spelling | doaj-art-ce7fa8060b8343e886da4412ea4f1d122025-08-20T03:37:23ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402025-08-01634020403410.21608/mid.2025.368471.2628422773Detection of plasmid-borne mcr genes conferring colistin resistance in MDR and XDR Gram-negative bacterial isolates from an Egyptian hospitalDoaa Yousef0Ahmed Soliman1Fatma Sonbol2Amal Abo Kamer3Hazem Ramadan4Mahmoud Farghali5Department of Microbiology and Immunology, Faculty of Pharmacy, Tanta University, Tanta 31527, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Kafr-Elsheikh University, Kafr-Elsheikh 33516, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Tanta University, Tanta 31527, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Tanta University, Tanta 31527, EgyptHygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Tanta University, Tanta 31527, EgyptBackground: Infections with multi-drug resistant (MDR) Gram-negative bacteria represent a serious public health risk, especially with the emergence of colistin resistance. Colistin resistance is mainly mediated by chromosomal mutations; however, there are reports of transferable plasmid-mediated colistin resistance genes, namely mcr, which have been universally documented. Thus, our study aimed to examine the incidence of mobile colistin resistance genes (mcr-1 and mcr-2) among MDR and extensively drug-resistant (XDR) Gram-negative bacteria. Methods Two hundred and forty-two Gram-negative clinical bacterial isolates were obtained in our study. Using standard microbiological methods, the bacteria were isolated and identified. Colistin resistance was phenotypically detected utilizing the broth microdilution technique. The colistin-resistant isolates were examined for their antimicrobial susceptibility profile using the Kirby-Bauer disk diffusion method. We used PCR to identify the mcr-1 and mcr-2 genes. Results Our data revealed that up to 18 (7.8%) isolates were colistin-resistant, including 11 Klebsiella spp. isolates, six Pseudomonas spp. isolates and only one Escherichia coli isolate. The PCR results revealed that mcr-1 was found in two isolates (11.1%), including one isolate of Klebsiella spp. (colistin MIC=32 µg/ml) and one isolate of Escherichia coli (colistin MIC=4 µg/ml). None of the colistin-resistant isolates carried mcr-2. Conclusions Based on our data, a relatively low incidence of colistin resistance was observed among clinical isolates. However, the detection of mcr-1 in two isolates of different species is concerning because of the possibility of spreading to susceptible strains. Public Health authorities should implement colistin resistance monitoring programs and infection control strategies in healthcare settings.https://mid.journals.ekb.eg/article_422773_5ee7e792e3f03b755d38724453bf162d.pdfmulti-drug resistant (mdr)colistin resistanceplasmid-mediated resistancemcr genesgram-negative bacteria |
| spellingShingle | Doaa Yousef Ahmed Soliman Fatma Sonbol Amal Abo Kamer Hazem Ramadan Mahmoud Farghali Detection of plasmid-borne mcr genes conferring colistin resistance in MDR and XDR Gram-negative bacterial isolates from an Egyptian hospital Microbes and Infectious Diseases multi-drug resistant (mdr) colistin resistance plasmid-mediated resistance mcr genes gram-negative bacteria |
| title | Detection of plasmid-borne mcr genes conferring colistin resistance in MDR and XDR Gram-negative bacterial isolates from an Egyptian hospital |
| title_full | Detection of plasmid-borne mcr genes conferring colistin resistance in MDR and XDR Gram-negative bacterial isolates from an Egyptian hospital |
| title_fullStr | Detection of plasmid-borne mcr genes conferring colistin resistance in MDR and XDR Gram-negative bacterial isolates from an Egyptian hospital |
| title_full_unstemmed | Detection of plasmid-borne mcr genes conferring colistin resistance in MDR and XDR Gram-negative bacterial isolates from an Egyptian hospital |
| title_short | Detection of plasmid-borne mcr genes conferring colistin resistance in MDR and XDR Gram-negative bacterial isolates from an Egyptian hospital |
| title_sort | detection of plasmid borne mcr genes conferring colistin resistance in mdr and xdr gram negative bacterial isolates from an egyptian hospital |
| topic | multi-drug resistant (mdr) colistin resistance plasmid-mediated resistance mcr genes gram-negative bacteria |
| url | https://mid.journals.ekb.eg/article_422773_5ee7e792e3f03b755d38724453bf162d.pdf |
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