Development of a Fluorescent Rapid Test Sensing System for Influenza Virus
This paper presents a sensitive and stable fluorescence rapid test sensing system for the quantitative analysis of influenza rapid test results, integrating a detection reader to minimize errors from conventional visual interpretation. The hardware includes a control board, touchscreen, camera modul...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-05-01
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| Online Access: | https://www.mdpi.com/2072-666X/16/6/635 |
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| author | Wei-Chien Weng Yu-Lin Wu Zia-Jia Lin Wen-Fung Pan Yu-Cheng Lin |
| author_facet | Wei-Chien Weng Yu-Lin Wu Zia-Jia Lin Wen-Fung Pan Yu-Cheng Lin |
| author_sort | Wei-Chien Weng |
| collection | DOAJ |
| description | This paper presents a sensitive and stable fluorescence rapid test sensing system for the quantitative analysis of influenza rapid test results, integrating a detection reader to minimize errors from conventional visual interpretation. The hardware includes a control board, touchscreen, camera module, UV LED illumination, and a dark chamber, while the software handles camera and light source control, as well as image processing. Validation shows strong linearity, high precision, and reproducibility. For influenza A (H1N1), the system achieved a coefficient of determination (R<sup>2</sup>) of 0.9782 (25–200 ng/mL) and 0.9865 (1–10 ng/mL); for influenza B (Yamagata), the coefficient of determination (R<sup>2</sup>) was 0.9762 (2–10 ng/mL). The coefficient of variation ranged from 1–5% for influenza A and 4–9% for influenza B. Detection limits were 4 ng/mL for influenza A and 6 ng/mL for influenza B. These results confirm the system’s capability for accurate quantitative analysis while reducing reliance on subjective interpretation. Its compact, portable design supports on-site rapid testing and allows for potential expansion to detect other targets, such as COVID-19, RSV, and myocardial enzymes. The system’s scalability makes it a promising tool for clinical diagnostics, point-of-care testing (POCT), and infectious disease monitoring. |
| format | Article |
| id | doaj-art-ce75ff82d1c34a1d9e360d6ac91ab2c8 |
| institution | Kabale University |
| issn | 2072-666X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj-art-ce75ff82d1c34a1d9e360d6ac91ab2c82025-08-20T03:27:40ZengMDPI AGMicromachines2072-666X2025-05-0116663510.3390/mi16060635Development of a Fluorescent Rapid Test Sensing System for Influenza VirusWei-Chien Weng0Yu-Lin Wu1Zia-Jia Lin2Wen-Fung Pan3Yu-Cheng Lin4Department of Engineering Science, National Cheng Kung University, Tainan 70101, TaiwanDepartment of Engineering Science, National Cheng Kung University, Tainan 70101, TaiwanDepartment of Engineering Science, National Cheng Kung University, Tainan 70101, TaiwanDepartment of Engineering Science, National Cheng Kung University, Tainan 70101, TaiwanDepartment of Engineering Science, National Cheng Kung University, Tainan 70101, TaiwanThis paper presents a sensitive and stable fluorescence rapid test sensing system for the quantitative analysis of influenza rapid test results, integrating a detection reader to minimize errors from conventional visual interpretation. The hardware includes a control board, touchscreen, camera module, UV LED illumination, and a dark chamber, while the software handles camera and light source control, as well as image processing. Validation shows strong linearity, high precision, and reproducibility. For influenza A (H1N1), the system achieved a coefficient of determination (R<sup>2</sup>) of 0.9782 (25–200 ng/mL) and 0.9865 (1–10 ng/mL); for influenza B (Yamagata), the coefficient of determination (R<sup>2</sup>) was 0.9762 (2–10 ng/mL). The coefficient of variation ranged from 1–5% for influenza A and 4–9% for influenza B. Detection limits were 4 ng/mL for influenza A and 6 ng/mL for influenza B. These results confirm the system’s capability for accurate quantitative analysis while reducing reliance on subjective interpretation. Its compact, portable design supports on-site rapid testing and allows for potential expansion to detect other targets, such as COVID-19, RSV, and myocardial enzymes. The system’s scalability makes it a promising tool for clinical diagnostics, point-of-care testing (POCT), and infectious disease monitoring.https://www.mdpi.com/2072-666X/16/6/635fluorescence immunoassay (FIA)image processinginfluenza detectionoptical detectionrapid test detectionsensing system |
| spellingShingle | Wei-Chien Weng Yu-Lin Wu Zia-Jia Lin Wen-Fung Pan Yu-Cheng Lin Development of a Fluorescent Rapid Test Sensing System for Influenza Virus Micromachines fluorescence immunoassay (FIA) image processing influenza detection optical detection rapid test detection sensing system |
| title | Development of a Fluorescent Rapid Test Sensing System for Influenza Virus |
| title_full | Development of a Fluorescent Rapid Test Sensing System for Influenza Virus |
| title_fullStr | Development of a Fluorescent Rapid Test Sensing System for Influenza Virus |
| title_full_unstemmed | Development of a Fluorescent Rapid Test Sensing System for Influenza Virus |
| title_short | Development of a Fluorescent Rapid Test Sensing System for Influenza Virus |
| title_sort | development of a fluorescent rapid test sensing system for influenza virus |
| topic | fluorescence immunoassay (FIA) image processing influenza detection optical detection rapid test detection sensing system |
| url | https://www.mdpi.com/2072-666X/16/6/635 |
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