Insulin in Myenteric Neurons: Time-Dependent and Regional Changes in Type 1 Diabetic Rats
Enteric neurons regulating motility display regional damage to diabetes. By inhibiting neuroinflammation, insulin can contribute to neuronal survival, therefore, we aimed to investigate the presence of insulin in myenteric neurons and their nitrergic population in acute and chronic rat models of typ...
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2025-05-01
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| author | Abigél Egyed-Kolumbán Benita Onhausz Bence Pál Barta Zita Szalai Ildikó Huliák Mónika Kiricsi Mária Bagyánszki Nikolett Bódi |
| author_facet | Abigél Egyed-Kolumbán Benita Onhausz Bence Pál Barta Zita Szalai Ildikó Huliák Mónika Kiricsi Mária Bagyánszki Nikolett Bódi |
| author_sort | Abigél Egyed-Kolumbán |
| collection | DOAJ |
| description | Enteric neurons regulating motility display regional damage to diabetes. By inhibiting neuroinflammation, insulin can contribute to neuronal survival, therefore, we aimed to investigate the presence of insulin in myenteric neurons and their nitrergic population in acute and chronic rat models of type 1 diabetes. One or ten weeks after the onset of hyperglycemia, gut segments and the pancreas of control, diabetic, and insulin-treated diabetic rats were investigated. In the controls, insulin-immunoreactive neurons comprised 8–9% of the total myenteric neuronal population in the ileum and colon and 2–4% in the duodenum. Except for the duodenum, this proportion was significantly increased in acute hyperglycemic rats and was decreased in the colon of the chronic ones. However, the proportion of insulin-immunoreactive nitrergic neurons remained unchanged in all segments in chronic hyperglycemia. Immunogold electron microscopy revealed an increased density of insulin-labelling gold particles in diabetic duodenal ganglia of the chronic experiment. Insulin mRNA was not detected in intestinal samples either in controls or diabetics. These findings support time-dependent and regional alterations in the proportion of insulin-immunoreactive myenteric neurons and their nitrergic subpopulation. Regionally different insulin content of myenteric neurons may contribute to their protection from diabetic damage. |
| format | Article |
| id | doaj-art-ce70a85bb5b140e1b24498d65b0fbb17 |
| institution | DOAJ |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-ce70a85bb5b140e1b24498d65b0fbb172025-08-20T03:11:30ZengMDPI AGCells2073-44092025-05-01141180910.3390/cells14110809Insulin in Myenteric Neurons: Time-Dependent and Regional Changes in Type 1 Diabetic RatsAbigél Egyed-Kolumbán0Benita Onhausz1Bence Pál Barta2Zita Szalai3Ildikó Huliák4Mónika Kiricsi5Mária Bagyánszki6Nikolett Bódi7Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryDepartment of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, Közép Fasor 52, H-6726 Szeged, HungaryEnteric neurons regulating motility display regional damage to diabetes. By inhibiting neuroinflammation, insulin can contribute to neuronal survival, therefore, we aimed to investigate the presence of insulin in myenteric neurons and their nitrergic population in acute and chronic rat models of type 1 diabetes. One or ten weeks after the onset of hyperglycemia, gut segments and the pancreas of control, diabetic, and insulin-treated diabetic rats were investigated. In the controls, insulin-immunoreactive neurons comprised 8–9% of the total myenteric neuronal population in the ileum and colon and 2–4% in the duodenum. Except for the duodenum, this proportion was significantly increased in acute hyperglycemic rats and was decreased in the colon of the chronic ones. However, the proportion of insulin-immunoreactive nitrergic neurons remained unchanged in all segments in chronic hyperglycemia. Immunogold electron microscopy revealed an increased density of insulin-labelling gold particles in diabetic duodenal ganglia of the chronic experiment. Insulin mRNA was not detected in intestinal samples either in controls or diabetics. These findings support time-dependent and regional alterations in the proportion of insulin-immunoreactive myenteric neurons and their nitrergic subpopulation. Regionally different insulin content of myenteric neurons may contribute to their protection from diabetic damage.https://www.mdpi.com/2073-4409/14/11/809animal modelshyperglycemiainsulinmyenteric neuronsneuronal insulin uptakeneuronal nitric oxide synthase |
| spellingShingle | Abigél Egyed-Kolumbán Benita Onhausz Bence Pál Barta Zita Szalai Ildikó Huliák Mónika Kiricsi Mária Bagyánszki Nikolett Bódi Insulin in Myenteric Neurons: Time-Dependent and Regional Changes in Type 1 Diabetic Rats Cells animal models hyperglycemia insulin myenteric neurons neuronal insulin uptake neuronal nitric oxide synthase |
| title | Insulin in Myenteric Neurons: Time-Dependent and Regional Changes in Type 1 Diabetic Rats |
| title_full | Insulin in Myenteric Neurons: Time-Dependent and Regional Changes in Type 1 Diabetic Rats |
| title_fullStr | Insulin in Myenteric Neurons: Time-Dependent and Regional Changes in Type 1 Diabetic Rats |
| title_full_unstemmed | Insulin in Myenteric Neurons: Time-Dependent and Regional Changes in Type 1 Diabetic Rats |
| title_short | Insulin in Myenteric Neurons: Time-Dependent and Regional Changes in Type 1 Diabetic Rats |
| title_sort | insulin in myenteric neurons time dependent and regional changes in type 1 diabetic rats |
| topic | animal models hyperglycemia insulin myenteric neurons neuronal insulin uptake neuronal nitric oxide synthase |
| url | https://www.mdpi.com/2073-4409/14/11/809 |
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