Altered gut microbiome in convalescent patients with coronavirus disease 2019
IntroductionCoronavirus disease 2019 (COVID-19) alters the gut microbiome. This study aimed to assess the association between the disease severity of COVID-19 and changes in stool microbes through a seven-month follow-up of stool collection.MethodsWe conducted a multicentre, prospective longitudinal...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1455295/full |
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| author | Kyoung Hwa Lee Yeong Ouk Kim Yeong Ouk Kim So Hee Dho Jen J. H. Yong Hyun-Seok Oh Je Hee Lee Seung-Jo Yang Inseong Cha Jongsik Chun Eun Hwa Lee Su Jin Jeong Wonjin Woo Jae-Phil Choi Sang Hoon Han Gloria B. Choi Jun R. Huh Jun R. Huh Lark Kyun Kim Young Goo Song |
| author_facet | Kyoung Hwa Lee Yeong Ouk Kim Yeong Ouk Kim So Hee Dho Jen J. H. Yong Hyun-Seok Oh Je Hee Lee Seung-Jo Yang Inseong Cha Jongsik Chun Eun Hwa Lee Su Jin Jeong Wonjin Woo Jae-Phil Choi Sang Hoon Han Gloria B. Choi Jun R. Huh Jun R. Huh Lark Kyun Kim Young Goo Song |
| author_sort | Kyoung Hwa Lee |
| collection | DOAJ |
| description | IntroductionCoronavirus disease 2019 (COVID-19) alters the gut microbiome. This study aimed to assess the association between the disease severity of COVID-19 and changes in stool microbes through a seven-month follow-up of stool collection.MethodsWe conducted a multicentre, prospective longitudinal study of 58 COVID-19 patients and 116 uninfected controls. Differences in the gut microbiota were analysed using 16S ribosomal RNA sequencing. The first stool samples were collected at an early convalescent phase of COVID-19, and the second sample was collected at least seven months after COVID-19 infection.Results and discussionAt the order level, Eubacteriales and Bifidobacteriales decreased, while Bacteroidales and Burkholderiales increased in the COVID-19 group compared to the controls. Alpha diversity also decreased in COVID-19 patients compared to controls, with imperfect recovery of the gut microbiome after seven months. The compositional change in the gut microbiome between the early and late convalescent phases was largest in the moderate and severe groups. The severity of COVID-19 was the most influential clinical variable for microbiome composition (Sum of Sqs = 0.686, P = 0.006), and its effect persisted even after partialling out other effects such as antibiotic use and age. Thus, our study indicates a possible interaction between respiratory viral infection and the composition of the gut microbiota community, warranting future mechanistic and prospective longitudinal studies. Additionally, we were able to detect microbiome changes in patients who were re-infected with SARS-CoV-2. Notably, the dominant bacteria in the re-infected group were Lachnospiraceae and Faecalimonas umbilicata, compared to the one-time infected group. |
| format | Article |
| id | doaj-art-ce6e6a620e1f4fa0883458e60e999df8 |
| institution | OA Journals |
| issn | 2235-2988 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-ce6e6a620e1f4fa0883458e60e999df82025-08-20T02:07:24ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882024-11-011410.3389/fcimb.2024.14552951455295Altered gut microbiome in convalescent patients with coronavirus disease 2019Kyoung Hwa Lee0Yeong Ouk Kim1Yeong Ouk Kim2So Hee Dho3Jen J. H. Yong4Hyun-Seok Oh5Je Hee Lee6Seung-Jo Yang7Inseong Cha8Jongsik Chun9Eun Hwa Lee10Su Jin Jeong11Wonjin Woo12Jae-Phil Choi13Sang Hoon Han14Gloria B. Choi15Jun R. Huh16Jun R. Huh17Lark Kyun Kim18Young Goo Song19Division of Infectious Diseases, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of KoreaCJ Bioscience, Inc., Seoul, Republic of KoreaInterdisciplinary Program in Bioinformatics, Seoul National University, Seoul, Republic of KoreaDepartment of Biomedical Sciences, Graduate School of Medical Science, Brain Korea 21 Project, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of KoreaDepartment of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA, United StatesCJ Bioscience, Inc., Seoul, Republic of KoreaCJ Bioscience, Inc., Seoul, Republic of KoreaCJ Bioscience, Inc., Seoul, Republic of KoreaCJ Bioscience, Inc., Seoul, Republic of KoreaCJ Bioscience, Inc., Seoul, Republic of KoreaDivision of Infectious Diseases, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of KoreaDivision of Infectious Diseases, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of KoreaDepartment of Biomedical Sciences, Graduate School of Medical Science, Brain Korea 21 Project, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of KoreaDivision of Infectious Diseases, Department of Internal Medicine, Seoul Medical Center, Seoul, Republic of KoreaDivision of Infectious Diseases, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of KoreaThe Picower Institute for Learning and Memory, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA, United StatesDepartment of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA, United StatesBio2Q, Keio University, Tokyo, JapanDepartment of Biomedical Sciences, Graduate School of Medical Science, Brain Korea 21 Project, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of KoreaDivision of Infectious Diseases, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Republic of KoreaIntroductionCoronavirus disease 2019 (COVID-19) alters the gut microbiome. This study aimed to assess the association between the disease severity of COVID-19 and changes in stool microbes through a seven-month follow-up of stool collection.MethodsWe conducted a multicentre, prospective longitudinal study of 58 COVID-19 patients and 116 uninfected controls. Differences in the gut microbiota were analysed using 16S ribosomal RNA sequencing. The first stool samples were collected at an early convalescent phase of COVID-19, and the second sample was collected at least seven months after COVID-19 infection.Results and discussionAt the order level, Eubacteriales and Bifidobacteriales decreased, while Bacteroidales and Burkholderiales increased in the COVID-19 group compared to the controls. Alpha diversity also decreased in COVID-19 patients compared to controls, with imperfect recovery of the gut microbiome after seven months. The compositional change in the gut microbiome between the early and late convalescent phases was largest in the moderate and severe groups. The severity of COVID-19 was the most influential clinical variable for microbiome composition (Sum of Sqs = 0.686, P = 0.006), and its effect persisted even after partialling out other effects such as antibiotic use and age. Thus, our study indicates a possible interaction between respiratory viral infection and the composition of the gut microbiota community, warranting future mechanistic and prospective longitudinal studies. Additionally, we were able to detect microbiome changes in patients who were re-infected with SARS-CoV-2. Notably, the dominant bacteria in the re-infected group were Lachnospiraceae and Faecalimonas umbilicata, compared to the one-time infected group.https://www.frontiersin.org/articles/10.3389/fcimb.2024.1455295/fullstool microbiomemetagenomicsSARS-CoV-2severity of coronavirus disease 2019 (COVID-19)convalescent patients |
| spellingShingle | Kyoung Hwa Lee Yeong Ouk Kim Yeong Ouk Kim So Hee Dho Jen J. H. Yong Hyun-Seok Oh Je Hee Lee Seung-Jo Yang Inseong Cha Jongsik Chun Eun Hwa Lee Su Jin Jeong Wonjin Woo Jae-Phil Choi Sang Hoon Han Gloria B. Choi Jun R. Huh Jun R. Huh Lark Kyun Kim Young Goo Song Altered gut microbiome in convalescent patients with coronavirus disease 2019 Frontiers in Cellular and Infection Microbiology stool microbiome metagenomics SARS-CoV-2 severity of coronavirus disease 2019 (COVID-19) convalescent patients |
| title | Altered gut microbiome in convalescent patients with coronavirus disease 2019 |
| title_full | Altered gut microbiome in convalescent patients with coronavirus disease 2019 |
| title_fullStr | Altered gut microbiome in convalescent patients with coronavirus disease 2019 |
| title_full_unstemmed | Altered gut microbiome in convalescent patients with coronavirus disease 2019 |
| title_short | Altered gut microbiome in convalescent patients with coronavirus disease 2019 |
| title_sort | altered gut microbiome in convalescent patients with coronavirus disease 2019 |
| topic | stool microbiome metagenomics SARS-CoV-2 severity of coronavirus disease 2019 (COVID-19) convalescent patients |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2024.1455295/full |
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