Production of Secretory Leucocyte Protease Inhibitor (SLPI) in Human Pancreatic β-Cells
Secretory leucocyte protease inhibitor (SLPI) is a potent inhibitor of granulocyte elastase and cathepsin G, and also an inhibitor of pancreatic enzymes like trypsin, chymotrypsin and pancreatic elastase. SLPI has also been shown to inhibit HIV-1 infections by blocking viral DNA synthesis. Since SLP...
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| Format: | Article |
| Language: | English |
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Wiley
1999-01-01
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| Series: | Mediators of Inflammation |
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| Online Access: | http://dx.doi.org/10.1080/09629359990478 |
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| author | Max Nyström Magnus Bergenfeldt Irena Ljungcrantz Èsa Lindeheim Kjell Ohlsson |
| author_facet | Max Nyström Magnus Bergenfeldt Irena Ljungcrantz Èsa Lindeheim Kjell Ohlsson |
| author_sort | Max Nyström |
| collection | DOAJ |
| description | Secretory leucocyte protease inhibitor (SLPI) is a potent inhibitor of granulocyte elastase and cathepsin G, and also an inhibitor of pancreatic enzymes like trypsin, chymotrypsin and pancreatic elastase. SLPI has also been shown to inhibit HIV-1 infections by blocking viral DNA synthesis. Since SLPI is an inhibitor of pancreatic proteases we wished to investigate whether SLPI was also actually produced in the pancreas. M-RNA from human pancreatic tissue showed evidence of SLPI production using the reverse transcriptase polymer chain reaction technique (RTPCR). Using immunohistochemical methods SLPI was demonstrated in the β-ce1ls of the islets of Langerhans. The function could be local protease/antiprotease regulation or antiviral/antibacterial defence in the close vicinity of the cell surface, or even inside the β-cell itself. |
| format | Article |
| id | doaj-art-ce66b01a41604bdf82ea91ea620ecb68 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1999-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-ce66b01a41604bdf82ea91ea620ecb682025-02-03T06:01:18ZengWileyMediators of Inflammation0962-93511466-18611999-01-018314715110.1080/09629359990478Production of Secretory Leucocyte Protease Inhibitor (SLPI) in Human Pancreatic β-CellsMax Nyström0Magnus Bergenfeldt1Irena Ljungcrantz2Èsa Lindeheim3Kjell Ohlsson4Department of Surgery, Lund University, Lund, Malmö University Hospital, Malmö SE-205 02, SwedenDepartment of Surgery, Lund University, Lund, Malmö University Hospital, Malmö SE-205 02, SwedenDepartment of Surgical Pathophysiology, Malmö University Hospital, Malmö SE-205 02, SwedenDepartment of Surgical Pathophysiology, Malmö University Hospital, Malmö SE-205 02, SwedenDepartment of Surgical Pathophysiology, Malmö University Hospital, Malmö SE-205 02, SwedenSecretory leucocyte protease inhibitor (SLPI) is a potent inhibitor of granulocyte elastase and cathepsin G, and also an inhibitor of pancreatic enzymes like trypsin, chymotrypsin and pancreatic elastase. SLPI has also been shown to inhibit HIV-1 infections by blocking viral DNA synthesis. Since SLPI is an inhibitor of pancreatic proteases we wished to investigate whether SLPI was also actually produced in the pancreas. M-RNA from human pancreatic tissue showed evidence of SLPI production using the reverse transcriptase polymer chain reaction technique (RTPCR). Using immunohistochemical methods SLPI was demonstrated in the β-ce1ls of the islets of Langerhans. The function could be local protease/antiprotease regulation or antiviral/antibacterial defence in the close vicinity of the cell surface, or even inside the β-cell itself.http://dx.doi.org/10.1080/09629359990478SLPIIslets of LangerhansPancreasβ-CellAntiviralAntibacterial. |
| spellingShingle | Max Nyström Magnus Bergenfeldt Irena Ljungcrantz Èsa Lindeheim Kjell Ohlsson Production of Secretory Leucocyte Protease Inhibitor (SLPI) in Human Pancreatic β-Cells Mediators of Inflammation SLPI Islets of Langerhans Pancreas β-Cell Antiviral Antibacterial. |
| title | Production of Secretory Leucocyte Protease Inhibitor (SLPI) in Human Pancreatic β-Cells |
| title_full | Production of Secretory Leucocyte Protease Inhibitor (SLPI) in Human Pancreatic β-Cells |
| title_fullStr | Production of Secretory Leucocyte Protease Inhibitor (SLPI) in Human Pancreatic β-Cells |
| title_full_unstemmed | Production of Secretory Leucocyte Protease Inhibitor (SLPI) in Human Pancreatic β-Cells |
| title_short | Production of Secretory Leucocyte Protease Inhibitor (SLPI) in Human Pancreatic β-Cells |
| title_sort | production of secretory leucocyte protease inhibitor slpi in human pancreatic β cells |
| topic | SLPI Islets of Langerhans Pancreas β-Cell Antiviral Antibacterial. |
| url | http://dx.doi.org/10.1080/09629359990478 |
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