PSAT1 regulates hair follicle growth and stem cell behavior in cashmere goats
Abstract Background The Arbas Cashmere Goat from Inner Mongolia is renowned for its superior-quality cashmere, which is primarily produced by secondary hair follicles (SHFs). Secondary hair follicle stem cells (SHFSCs) are critical regulators of SHF growth and development. However, the specific regu...
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BMC
2025-04-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-025-04736-6 |
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| author | Xiao-yu Han Jia-ning Liu Nan-xiang Sun Yin-xian Zhang Hao-bing Bai Wei-guo Song Xiao Hu Hao Liang Xiong Miao Yun-mei He Dong-jun Liu Xu-dong Guo |
| author_facet | Xiao-yu Han Jia-ning Liu Nan-xiang Sun Yin-xian Zhang Hao-bing Bai Wei-guo Song Xiao Hu Hao Liang Xiong Miao Yun-mei He Dong-jun Liu Xu-dong Guo |
| author_sort | Xiao-yu Han |
| collection | DOAJ |
| description | Abstract Background The Arbas Cashmere Goat from Inner Mongolia is renowned for its superior-quality cashmere, which is primarily produced by secondary hair follicles (SHFs). Secondary hair follicle stem cells (SHFSCs) are critical regulators of SHF growth and development. However, the specific regulatory mechanisms of phosphoserine aminotransferase 1 (PSAT1) in SHFSCs remain unclear. This study aimed to examine the expression pattern of the PSAT1 gene during SHF cycle transitions in cashmere goats and analyze its effects on SHFSC survival and wound healing. Results PSAT1 expression was significantly higher in the anagen phase than in the telogen phase, and was predominantly localized to the bulge region. Functional analyses revealed that elevated PSAT1 expression inhibited SHFSC survival and delayed wound healing; on the other hand, a reduced expression promoted SHFSC survival and accelerated healing. Transcriptomic profiling further demonstrated that PSAT1 expression levels markedly altered the gene expression landscape of SHFSCs. Notably, key signaling pathways essential for hair follicle growth and development, such as Wnt/β-catenin, MAPK, and TGF-β, were significantly affected by PSAT1 modulation. Conclusions This study highlights PSAT1 as a critical regulator of SHFSC function in cashmere goats, affecting both cellular survival and regenerative capacity. Through its modulation of multiple signaling pathways, PSAT1 plays a pivotal role in the SHF cycle and may serve as a potential molecular target for improving cashmere fiber production. |
| format | Article |
| id | doaj-art-ce5ebc12dac046bf9e88803f03e7e031 |
| institution | OA Journals |
| issn | 1746-6148 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj-art-ce5ebc12dac046bf9e88803f03e7e0312025-08-20T02:17:50ZengBMCBMC Veterinary Research1746-61482025-04-0121111510.1186/s12917-025-04736-6PSAT1 regulates hair follicle growth and stem cell behavior in cashmere goatsXiao-yu Han0Jia-ning Liu1Nan-xiang Sun2Yin-xian Zhang3Hao-bing Bai4Wei-guo Song5Xiao Hu6Hao Liang7Xiong Miao8Yun-mei He9Dong-jun Liu10Xu-dong Guo11Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia UniversityKey Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia UniversityKey Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia UniversityKey Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia UniversityKey Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia UniversityKey Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia UniversityKey Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia UniversityKey Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia UniversityAgriculture and Animal Husbandry Technology Extension CenterAgriculture and Animal Husbandry Technology Extension CenterKey Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia UniversityKey Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia UniversityAbstract Background The Arbas Cashmere Goat from Inner Mongolia is renowned for its superior-quality cashmere, which is primarily produced by secondary hair follicles (SHFs). Secondary hair follicle stem cells (SHFSCs) are critical regulators of SHF growth and development. However, the specific regulatory mechanisms of phosphoserine aminotransferase 1 (PSAT1) in SHFSCs remain unclear. This study aimed to examine the expression pattern of the PSAT1 gene during SHF cycle transitions in cashmere goats and analyze its effects on SHFSC survival and wound healing. Results PSAT1 expression was significantly higher in the anagen phase than in the telogen phase, and was predominantly localized to the bulge region. Functional analyses revealed that elevated PSAT1 expression inhibited SHFSC survival and delayed wound healing; on the other hand, a reduced expression promoted SHFSC survival and accelerated healing. Transcriptomic profiling further demonstrated that PSAT1 expression levels markedly altered the gene expression landscape of SHFSCs. Notably, key signaling pathways essential for hair follicle growth and development, such as Wnt/β-catenin, MAPK, and TGF-β, were significantly affected by PSAT1 modulation. Conclusions This study highlights PSAT1 as a critical regulator of SHFSC function in cashmere goats, affecting both cellular survival and regenerative capacity. Through its modulation of multiple signaling pathways, PSAT1 plays a pivotal role in the SHF cycle and may serve as a potential molecular target for improving cashmere fiber production.https://doi.org/10.1186/s12917-025-04736-6CashmereSecondary hair folliclesSecondary hair follicle stem cellsPhosphoserine aminotransferase 1Cell survivalWound healing |
| spellingShingle | Xiao-yu Han Jia-ning Liu Nan-xiang Sun Yin-xian Zhang Hao-bing Bai Wei-guo Song Xiao Hu Hao Liang Xiong Miao Yun-mei He Dong-jun Liu Xu-dong Guo PSAT1 regulates hair follicle growth and stem cell behavior in cashmere goats BMC Veterinary Research Cashmere Secondary hair follicles Secondary hair follicle stem cells Phosphoserine aminotransferase 1 Cell survival Wound healing |
| title | PSAT1 regulates hair follicle growth and stem cell behavior in cashmere goats |
| title_full | PSAT1 regulates hair follicle growth and stem cell behavior in cashmere goats |
| title_fullStr | PSAT1 regulates hair follicle growth and stem cell behavior in cashmere goats |
| title_full_unstemmed | PSAT1 regulates hair follicle growth and stem cell behavior in cashmere goats |
| title_short | PSAT1 regulates hair follicle growth and stem cell behavior in cashmere goats |
| title_sort | psat1 regulates hair follicle growth and stem cell behavior in cashmere goats |
| topic | Cashmere Secondary hair follicles Secondary hair follicle stem cells Phosphoserine aminotransferase 1 Cell survival Wound healing |
| url | https://doi.org/10.1186/s12917-025-04736-6 |
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