Prolonged sitting is not associated with altered shear‐mediated dilation of the internal carotid artery, despite impairing lower limb endothelial function
Abstract The present study aims to examine the effect of 4 h of continuous sitting on cerebral endothelial function, which is a crucial component of cerebral blood flow regulation. We hypothesized that 4 h of sitting may impair cerebral endothelial function similarly to how it affects lower limb vas...
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Wiley
2025-02-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.70097 |
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| author | Shotaro Saito Kento Dora Marino Karaki Narumi Kunimatsu Hayato Tsukamoto Jun Sugawara Erika Iwamoto Shigehiko Ogoh |
| author_facet | Shotaro Saito Kento Dora Marino Karaki Narumi Kunimatsu Hayato Tsukamoto Jun Sugawara Erika Iwamoto Shigehiko Ogoh |
| author_sort | Shotaro Saito |
| collection | DOAJ |
| description | Abstract The present study aims to examine the effect of 4 h of continuous sitting on cerebral endothelial function, which is a crucial component of cerebral blood flow regulation. We hypothesized that 4 h of sitting may impair cerebral endothelial function similarly to how it affects lower limb vasculature. Thirteen young, healthy participants were instructed to remain seated for 4 h without moving their lower limbs. The blood flow and shear rate (SR) in the popliteal and internal carotid artery (ICA) were measured using duplex Doppler ultrasound. During the 4‐h sitting, peripheral (popliteal artery) and cerebral (ICA) endothelial function were assessed every hour. We induced peripheral and cerebral flow‐mediated dilation (pFMD and ICA FMD) using hyperemia (5 min of cuff inflation on lower limb, then deflation) or hypercapnia (30s of hypercapnia, end‐tidal partial pressure of CO2 + 9 mmHg), respectively. We then calculated each relative peak dilation from the baseline diameter to identify both pFMD and ICA FMD. We observed a significant decrease in pFMD starting at 2 h from the onset of sitting, and this reduction persisted throughout the 4‐h sitting [Base (6.8 ± 4.2%) vs. 2‐h (3.9 ± 2.0%), p = 0.044; vs. 3‐h (3.2 ± 1.8%), p = 0.016; vs. 4‐h (3.2 ± 1.9%), p = 0.005]. In contrast, during the 4‐h sitting, ICA blood flow, SR, and ICA FMD remained unchanged (p = 0.062, p = 0.068, and p = 0.203, respectively). Unlike peripheral endothelial function, cerebral endothelial function remained stable during 4‐h sitting. This suggests that the acute effect of prolonged sitting on cerebral vasculature differs from that of lower limb vasculature. |
| format | Article |
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| institution | OA Journals |
| issn | 2051-817X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wiley |
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| series | Physiological Reports |
| spelling | doaj-art-ce55e7b9a7e341b2a6c8f3e15ae6fec92025-08-20T02:31:08ZengWileyPhysiological Reports2051-817X2025-02-01133n/an/a10.14814/phy2.70097Prolonged sitting is not associated with altered shear‐mediated dilation of the internal carotid artery, despite impairing lower limb endothelial functionShotaro Saito0Kento Dora1Marino Karaki2Narumi Kunimatsu3Hayato Tsukamoto4Jun Sugawara5Erika Iwamoto6Shigehiko Ogoh7Department of Biomedical Engineering Toyo University Saitama JapanDepartment of Biomedical Engineering Toyo University Saitama JapanGraduate School of Health and Sport Sciences Chukyo University Aichi JapanDepartment of Biomedical Engineering Toyo University Saitama JapanFaculty of Sport Sciences Waseda University Saitama JapanHuman Informatics and Interaction Research Institute National Institute of Advanced Industrial Science and Technology Ibaraki JapanSchool of Health Sciences Sapporo Medical University Sapporo Hokkaido JapanDepartment of Biomedical Engineering Toyo University Saitama JapanAbstract The present study aims to examine the effect of 4 h of continuous sitting on cerebral endothelial function, which is a crucial component of cerebral blood flow regulation. We hypothesized that 4 h of sitting may impair cerebral endothelial function similarly to how it affects lower limb vasculature. Thirteen young, healthy participants were instructed to remain seated for 4 h without moving their lower limbs. The blood flow and shear rate (SR) in the popliteal and internal carotid artery (ICA) were measured using duplex Doppler ultrasound. During the 4‐h sitting, peripheral (popliteal artery) and cerebral (ICA) endothelial function were assessed every hour. We induced peripheral and cerebral flow‐mediated dilation (pFMD and ICA FMD) using hyperemia (5 min of cuff inflation on lower limb, then deflation) or hypercapnia (30s of hypercapnia, end‐tidal partial pressure of CO2 + 9 mmHg), respectively. We then calculated each relative peak dilation from the baseline diameter to identify both pFMD and ICA FMD. We observed a significant decrease in pFMD starting at 2 h from the onset of sitting, and this reduction persisted throughout the 4‐h sitting [Base (6.8 ± 4.2%) vs. 2‐h (3.9 ± 2.0%), p = 0.044; vs. 3‐h (3.2 ± 1.8%), p = 0.016; vs. 4‐h (3.2 ± 1.9%), p = 0.005]. In contrast, during the 4‐h sitting, ICA blood flow, SR, and ICA FMD remained unchanged (p = 0.062, p = 0.068, and p = 0.203, respectively). Unlike peripheral endothelial function, cerebral endothelial function remained stable during 4‐h sitting. This suggests that the acute effect of prolonged sitting on cerebral vasculature differs from that of lower limb vasculature.https://doi.org/10.14814/phy2.70097cerebral blood flowcerebrovascular shear rateinternal carotid arterysedentarism |
| spellingShingle | Shotaro Saito Kento Dora Marino Karaki Narumi Kunimatsu Hayato Tsukamoto Jun Sugawara Erika Iwamoto Shigehiko Ogoh Prolonged sitting is not associated with altered shear‐mediated dilation of the internal carotid artery, despite impairing lower limb endothelial function Physiological Reports cerebral blood flow cerebrovascular shear rate internal carotid artery sedentarism |
| title | Prolonged sitting is not associated with altered shear‐mediated dilation of the internal carotid artery, despite impairing lower limb endothelial function |
| title_full | Prolonged sitting is not associated with altered shear‐mediated dilation of the internal carotid artery, despite impairing lower limb endothelial function |
| title_fullStr | Prolonged sitting is not associated with altered shear‐mediated dilation of the internal carotid artery, despite impairing lower limb endothelial function |
| title_full_unstemmed | Prolonged sitting is not associated with altered shear‐mediated dilation of the internal carotid artery, despite impairing lower limb endothelial function |
| title_short | Prolonged sitting is not associated with altered shear‐mediated dilation of the internal carotid artery, despite impairing lower limb endothelial function |
| title_sort | prolonged sitting is not associated with altered shear mediated dilation of the internal carotid artery despite impairing lower limb endothelial function |
| topic | cerebral blood flow cerebrovascular shear rate internal carotid artery sedentarism |
| url | https://doi.org/10.14814/phy2.70097 |
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