Allele specific CAPS marker development and characterization of chalcone synthase gene in Indian mulberry (Morus spp., family Moraceae).
Chalcone synthase (CHS) is an essential enzyme in the phenylpropanoid pathway that catalyzes the first step in flavonoid biosynthesis in plants under diverse environmental stress. We have used CHS as a candidate gene in mulberry and developed Single Nucleotide Polymorphism (SNP) based co-dominant Cl...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179189&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850230489781108736 |
|---|---|
| author | Vivek Arora M K Ghosh Soumili Pal Gaurab Gangopadhyay |
| author_facet | Vivek Arora M K Ghosh Soumili Pal Gaurab Gangopadhyay |
| author_sort | Vivek Arora |
| collection | DOAJ |
| description | Chalcone synthase (CHS) is an essential enzyme in the phenylpropanoid pathway that catalyzes the first step in flavonoid biosynthesis in plants under diverse environmental stress. We have used CHS as a candidate gene in mulberry and developed Single Nucleotide Polymorphism (SNP) based co-dominant Cleaved Amplified Polymorphic Sequence (CAPS) marker associated with the CHS locus. The segregation pattern of the marker was studied in an F1 population derived from a hybridization program between two mulberry genotypes showing polymorphism for the CHS locus. Differential CHS activity of the recombinants has been correlated with the segregation pattern of the marker. Homology modelling and docking studies are performed for both the identified CHS alleles and correlated with respective CHS activity. Phenotyping of Powdery Mildew infected F1 population indicated a probable association with the CAPS marker. |
| format | Article |
| id | doaj-art-ce525083ad0549d7b75d58bae4d5adba |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-ce525083ad0549d7b75d58bae4d5adba2025-08-20T02:03:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01126e017918910.1371/journal.pone.0179189Allele specific CAPS marker development and characterization of chalcone synthase gene in Indian mulberry (Morus spp., family Moraceae).Vivek AroraM K GhoshSoumili PalGaurab GangopadhyayChalcone synthase (CHS) is an essential enzyme in the phenylpropanoid pathway that catalyzes the first step in flavonoid biosynthesis in plants under diverse environmental stress. We have used CHS as a candidate gene in mulberry and developed Single Nucleotide Polymorphism (SNP) based co-dominant Cleaved Amplified Polymorphic Sequence (CAPS) marker associated with the CHS locus. The segregation pattern of the marker was studied in an F1 population derived from a hybridization program between two mulberry genotypes showing polymorphism for the CHS locus. Differential CHS activity of the recombinants has been correlated with the segregation pattern of the marker. Homology modelling and docking studies are performed for both the identified CHS alleles and correlated with respective CHS activity. Phenotyping of Powdery Mildew infected F1 population indicated a probable association with the CAPS marker.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179189&type=printable |
| spellingShingle | Vivek Arora M K Ghosh Soumili Pal Gaurab Gangopadhyay Allele specific CAPS marker development and characterization of chalcone synthase gene in Indian mulberry (Morus spp., family Moraceae). PLoS ONE |
| title | Allele specific CAPS marker development and characterization of chalcone synthase gene in Indian mulberry (Morus spp., family Moraceae). |
| title_full | Allele specific CAPS marker development and characterization of chalcone synthase gene in Indian mulberry (Morus spp., family Moraceae). |
| title_fullStr | Allele specific CAPS marker development and characterization of chalcone synthase gene in Indian mulberry (Morus spp., family Moraceae). |
| title_full_unstemmed | Allele specific CAPS marker development and characterization of chalcone synthase gene in Indian mulberry (Morus spp., family Moraceae). |
| title_short | Allele specific CAPS marker development and characterization of chalcone synthase gene in Indian mulberry (Morus spp., family Moraceae). |
| title_sort | allele specific caps marker development and characterization of chalcone synthase gene in indian mulberry morus spp family moraceae |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0179189&type=printable |
| work_keys_str_mv | AT vivekarora allelespecificcapsmarkerdevelopmentandcharacterizationofchalconesynthasegeneinindianmulberrymorussppfamilymoraceae AT mkghosh allelespecificcapsmarkerdevelopmentandcharacterizationofchalconesynthasegeneinindianmulberrymorussppfamilymoraceae AT soumilipal allelespecificcapsmarkerdevelopmentandcharacterizationofchalconesynthasegeneinindianmulberrymorussppfamilymoraceae AT gaurabgangopadhyay allelespecificcapsmarkerdevelopmentandcharacterizationofchalconesynthasegeneinindianmulberrymorussppfamilymoraceae |