The Relationship Between ADAMTS‐4 and ADAMTS‐5 Enzyme Levels in Patients With Degenerative Disc Disease: A Prospective Biochemical Study
ABSTRACT Study Design Prospective biochemical study of comparison of A Disintegrin and Metalloproteinase with Thrombospondin motifs‐4 (ADAMTS‐4) and A Disintegrin and Metalloproteinase with Thrombospondin motifs 5 (ADAMTS5) levels in preoperative and postoperative venous blood, as well as in disc ti...
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Wiley
2025-03-01
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| Online Access: | https://doi.org/10.1002/jsp2.70037 |
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| author | Cansu Tuncer Emin Mehmet Eminoğlu Ömer Emre Yağlı Gülçin Baş Ahmet Sancar Topal Ece Onur Habib Özdemir Şemsi Güliz Yılmaz Gülgün Yılmaz Ovalı Cüneyt Temiz |
| author_facet | Cansu Tuncer Emin Mehmet Eminoğlu Ömer Emre Yağlı Gülçin Baş Ahmet Sancar Topal Ece Onur Habib Özdemir Şemsi Güliz Yılmaz Gülgün Yılmaz Ovalı Cüneyt Temiz |
| author_sort | Cansu Tuncer |
| collection | DOAJ |
| description | ABSTRACT Study Design Prospective biochemical study of comparison of A Disintegrin and Metalloproteinase with Thrombospondin motifs‐4 (ADAMTS‐4) and A Disintegrin and Metalloproteinase with Thrombospondin motifs 5 (ADAMTS5) levels in preoperative and postoperative venous blood, as well as in disc tissue obtained during surgery, in patients undergoing surgery for intervertebral disc disease, with enzyme levels in venous blood from a control group. Objective To compare the levels of ADAMTS‐4 and ADAMTS‐5 between patients with degenerative intervertebral discs and a healthy control group, aiming to identify biomarkers associated with intervertebral disc degeneration. Literature Although numerous studies have investigated the relationship between ADAMTS‐4 and ADAMTS‐5 enzymes and degeneration in experimental rat models and human tissues, no study has correlated their serum levels with intervertebral disc degeneration. Method and Materials Venous blood samples were obtained preoperatively and postoperatively from 41 patients (age: 42 ± 9.7 years, range 20–63) diagnosed with intervertebral disc disease. The affected disc levels were L4‐L5 in 22 patients and L5‐S1 in 19 patients. These patients were selected based on surgical indications due to radicular pain that persisted after an adequate course of conservative management, without any non‐neurological deficit. Disc tissue samples were also obtained during surgery. Additionally, venous blood samples were collected from a control group with no diagnosed diseases, and lumbar MRIs of the control group showed no significant signs of degeneration. ADAMTS‐4 and ADAMTS‐5 levels were measured using the ELISA method on samples obtained after centrifugation of the collected blood and tissue specimens. Results The level of ADAMTS‐4 in patient serum was found to be lower compared to the control group, while the level of ADAMTS‐5 was higher in the patient serum and lower in the control group. Conclusion Elevated levels of ADAMTS‐5 in the blood may be associated with intervertebral disc degeneration. |
| format | Article |
| id | doaj-art-ce4940d10fb54776a4f0b11cf6adbb94 |
| institution | DOAJ |
| issn | 2572-1143 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
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| series | JOR Spine |
| spelling | doaj-art-ce4940d10fb54776a4f0b11cf6adbb942025-08-20T03:11:04ZengWileyJOR Spine2572-11432025-03-0181n/an/a10.1002/jsp2.70037The Relationship Between ADAMTS‐4 and ADAMTS‐5 Enzyme Levels in Patients With Degenerative Disc Disease: A Prospective Biochemical StudyCansu Tuncer0Emin Mehmet Eminoğlu1Ömer Emre Yağlı2Gülçin Baş3Ahmet Sancar Topal4Ece Onur5Habib Özdemir6Şemsi Güliz Yılmaz7Gülgün Yılmaz Ovalı8Cüneyt Temiz9Department of Neurosurgery, Sorgun State Hospital Yozgat TurkeyDepartment of Neurosurgery Celal Bayar University, Faculty of Medicine Manisa TurkeyDepartment of Neurosurgery, Grandmedical Hospital Manisa TurkeyDepartment of Neurosurgery İstanbul University‐Cerrahpaşa Cerrahpaşa Faculty of Medicine İstanbul TurkeyDepartment of Neurosurgery, Turhal State Hospital Tokat TurkeyDepartment of Medical Biochemistry Celal Bayar University, Faculty of Medicine Manisa TurkeyDepartment of Medical Biochemistry Celal Bayar University, Faculty of Medicine Manisa TurkeyDepartment of Radiology Celal Bayar University, Faculty of Medicine Manisa TurkeyDepartment of Radiology Celal Bayar University, Faculty of Medicine Manisa TurkeyDepartment of Neurosurgery Celal Bayar University, Faculty of Medicine Manisa TurkeyABSTRACT Study Design Prospective biochemical study of comparison of A Disintegrin and Metalloproteinase with Thrombospondin motifs‐4 (ADAMTS‐4) and A Disintegrin and Metalloproteinase with Thrombospondin motifs 5 (ADAMTS5) levels in preoperative and postoperative venous blood, as well as in disc tissue obtained during surgery, in patients undergoing surgery for intervertebral disc disease, with enzyme levels in venous blood from a control group. Objective To compare the levels of ADAMTS‐4 and ADAMTS‐5 between patients with degenerative intervertebral discs and a healthy control group, aiming to identify biomarkers associated with intervertebral disc degeneration. Literature Although numerous studies have investigated the relationship between ADAMTS‐4 and ADAMTS‐5 enzymes and degeneration in experimental rat models and human tissues, no study has correlated their serum levels with intervertebral disc degeneration. Method and Materials Venous blood samples were obtained preoperatively and postoperatively from 41 patients (age: 42 ± 9.7 years, range 20–63) diagnosed with intervertebral disc disease. The affected disc levels were L4‐L5 in 22 patients and L5‐S1 in 19 patients. These patients were selected based on surgical indications due to radicular pain that persisted after an adequate course of conservative management, without any non‐neurological deficit. Disc tissue samples were also obtained during surgery. Additionally, venous blood samples were collected from a control group with no diagnosed diseases, and lumbar MRIs of the control group showed no significant signs of degeneration. ADAMTS‐4 and ADAMTS‐5 levels were measured using the ELISA method on samples obtained after centrifugation of the collected blood and tissue specimens. Results The level of ADAMTS‐4 in patient serum was found to be lower compared to the control group, while the level of ADAMTS‐5 was higher in the patient serum and lower in the control group. Conclusion Elevated levels of ADAMTS‐5 in the blood may be associated with intervertebral disc degeneration.https://doi.org/10.1002/jsp2.70037ADAMTS‐4ADAMTS‐5biomarkerslumbar disc degeneration |
| spellingShingle | Cansu Tuncer Emin Mehmet Eminoğlu Ömer Emre Yağlı Gülçin Baş Ahmet Sancar Topal Ece Onur Habib Özdemir Şemsi Güliz Yılmaz Gülgün Yılmaz Ovalı Cüneyt Temiz The Relationship Between ADAMTS‐4 and ADAMTS‐5 Enzyme Levels in Patients With Degenerative Disc Disease: A Prospective Biochemical Study JOR Spine ADAMTS‐4 ADAMTS‐5 biomarkers lumbar disc degeneration |
| title | The Relationship Between ADAMTS‐4 and ADAMTS‐5 Enzyme Levels in Patients With Degenerative Disc Disease: A Prospective Biochemical Study |
| title_full | The Relationship Between ADAMTS‐4 and ADAMTS‐5 Enzyme Levels in Patients With Degenerative Disc Disease: A Prospective Biochemical Study |
| title_fullStr | The Relationship Between ADAMTS‐4 and ADAMTS‐5 Enzyme Levels in Patients With Degenerative Disc Disease: A Prospective Biochemical Study |
| title_full_unstemmed | The Relationship Between ADAMTS‐4 and ADAMTS‐5 Enzyme Levels in Patients With Degenerative Disc Disease: A Prospective Biochemical Study |
| title_short | The Relationship Between ADAMTS‐4 and ADAMTS‐5 Enzyme Levels in Patients With Degenerative Disc Disease: A Prospective Biochemical Study |
| title_sort | relationship between adamts 4 and adamts 5 enzyme levels in patients with degenerative disc disease a prospective biochemical study |
| topic | ADAMTS‐4 ADAMTS‐5 biomarkers lumbar disc degeneration |
| url | https://doi.org/10.1002/jsp2.70037 |
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