Exploring the Molecular Mechanisms of Autophagy-related Genes in Hepatic Ischemia/Reperfusion Injury Using Bioinformatics
Background. Autophagy is a highly conserved cellular process. In the context of hepatic ischemia/reperfusion injury (HIRI), dysregulation of autophagy may lead to hepatocyte dysfunction. Therefore, we conducted a comprehensive transcriptomics analysis to investigate the biomolecular mechanisms under...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer
2025-07-01
|
| Series: | Transplantation Direct |
| Online Access: | http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000001829 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849304238385004544 |
|---|---|
| author | Qi Xiao, PhD Xiaoxiao Hu, MD Qiong Chen, MD WenYu Wang, MD JianSheng Xiao, PhD Biqi Fu, PhD |
| author_facet | Qi Xiao, PhD Xiaoxiao Hu, MD Qiong Chen, MD WenYu Wang, MD JianSheng Xiao, PhD Biqi Fu, PhD |
| author_sort | Qi Xiao, PhD |
| collection | DOAJ |
| description | Background. Autophagy is a highly conserved cellular process. In the context of hepatic ischemia/reperfusion injury (HIRI), dysregulation of autophagy may lead to hepatocyte dysfunction. Therefore, we conducted a comprehensive transcriptomics analysis to investigate the biomolecular mechanisms underlying autophagy in HIRI.
Methods. Bioinformatics were used to analyze the GSE112713 data set, with the objective of identifying the differential expression of autophagy-related genes (DEARGs). The expression and diagnostic potential of DEARGs were validated using in vitro models and receiver operating characteristic curves. Additionally, potential therapeutic drugs targeting DEARGs were predicted.
Results. Transcriptome bioinformatics analysis revealed widespread dysregulation of autophagy in HIRI. Seven DEARGs (IL6, JUN, HSPA1A, PPP1R15A, ERN1, DNAJB1, and HSPA1B) were confirmed in vitro. Based on these findings, we predicted potential drugs that may mitigate HIRI by modulating autophagy.
Conclusions. The present study identified 7 DEARGs (IL6, JUN, HSPA1A, PPP1R15A, ERN1, DNAJB1, and HSPA1B) in HIRI, which provides a reliable therapeutic target for HIRI. |
| format | Article |
| id | doaj-art-ce476acf8a174be0b3da3d9add25bc4d |
| institution | Kabale University |
| issn | 2373-8731 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wolters Kluwer |
| record_format | Article |
| series | Transplantation Direct |
| spelling | doaj-art-ce476acf8a174be0b3da3d9add25bc4d2025-08-20T03:55:48ZengWolters KluwerTransplantation Direct2373-87312025-07-01117e182910.1097/TXD.0000000000001829202507000-00010Exploring the Molecular Mechanisms of Autophagy-related Genes in Hepatic Ischemia/Reperfusion Injury Using BioinformaticsQi Xiao, PhD0Xiaoxiao Hu, MD1Qiong Chen, MD2WenYu Wang, MD3JianSheng Xiao, PhD4Biqi Fu, PhD51 Department of Transplantation, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nan Chang, Jiangxi, China.1 Department of Transplantation, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nan Chang, Jiangxi, China.1 Department of Transplantation, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nan Chang, Jiangxi, China.1 Department of Transplantation, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nan Chang, Jiangxi, China.1 Department of Transplantation, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nan Chang, Jiangxi, China.2 Department of Immunology, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nan Chang, Jiangxi, China.Background. Autophagy is a highly conserved cellular process. In the context of hepatic ischemia/reperfusion injury (HIRI), dysregulation of autophagy may lead to hepatocyte dysfunction. Therefore, we conducted a comprehensive transcriptomics analysis to investigate the biomolecular mechanisms underlying autophagy in HIRI. Methods. Bioinformatics were used to analyze the GSE112713 data set, with the objective of identifying the differential expression of autophagy-related genes (DEARGs). The expression and diagnostic potential of DEARGs were validated using in vitro models and receiver operating characteristic curves. Additionally, potential therapeutic drugs targeting DEARGs were predicted. Results. Transcriptome bioinformatics analysis revealed widespread dysregulation of autophagy in HIRI. Seven DEARGs (IL6, JUN, HSPA1A, PPP1R15A, ERN1, DNAJB1, and HSPA1B) were confirmed in vitro. Based on these findings, we predicted potential drugs that may mitigate HIRI by modulating autophagy. Conclusions. The present study identified 7 DEARGs (IL6, JUN, HSPA1A, PPP1R15A, ERN1, DNAJB1, and HSPA1B) in HIRI, which provides a reliable therapeutic target for HIRI.http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000001829 |
| spellingShingle | Qi Xiao, PhD Xiaoxiao Hu, MD Qiong Chen, MD WenYu Wang, MD JianSheng Xiao, PhD Biqi Fu, PhD Exploring the Molecular Mechanisms of Autophagy-related Genes in Hepatic Ischemia/Reperfusion Injury Using Bioinformatics Transplantation Direct |
| title | Exploring the Molecular Mechanisms of Autophagy-related Genes in Hepatic Ischemia/Reperfusion Injury Using Bioinformatics |
| title_full | Exploring the Molecular Mechanisms of Autophagy-related Genes in Hepatic Ischemia/Reperfusion Injury Using Bioinformatics |
| title_fullStr | Exploring the Molecular Mechanisms of Autophagy-related Genes in Hepatic Ischemia/Reperfusion Injury Using Bioinformatics |
| title_full_unstemmed | Exploring the Molecular Mechanisms of Autophagy-related Genes in Hepatic Ischemia/Reperfusion Injury Using Bioinformatics |
| title_short | Exploring the Molecular Mechanisms of Autophagy-related Genes in Hepatic Ischemia/Reperfusion Injury Using Bioinformatics |
| title_sort | exploring the molecular mechanisms of autophagy related genes in hepatic ischemia reperfusion injury using bioinformatics |
| url | http://journals.lww.com/transplantationdirect/fulltext/10.1097/TXD.0000000000001829 |
| work_keys_str_mv | AT qixiaophd exploringthemolecularmechanismsofautophagyrelatedgenesinhepaticischemiareperfusioninjuryusingbioinformatics AT xiaoxiaohumd exploringthemolecularmechanismsofautophagyrelatedgenesinhepaticischemiareperfusioninjuryusingbioinformatics AT qiongchenmd exploringthemolecularmechanismsofautophagyrelatedgenesinhepaticischemiareperfusioninjuryusingbioinformatics AT wenyuwangmd exploringthemolecularmechanismsofautophagyrelatedgenesinhepaticischemiareperfusioninjuryusingbioinformatics AT jianshengxiaophd exploringthemolecularmechanismsofautophagyrelatedgenesinhepaticischemiareperfusioninjuryusingbioinformatics AT biqifuphd exploringthemolecularmechanismsofautophagyrelatedgenesinhepaticischemiareperfusioninjuryusingbioinformatics |