Atmospheric pressure plasma treatment increases the antioxidant capacity of the serum in vitro
Abstract The interaction of atmospheric pressure plasma (APP) with the liquids has been fully studied, and multiple biomedical applications of APP-activated liquids have also been verified. However, there is still no direct evidence regarding the interaction between APP treatment and serum. In the p...
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Nature Portfolio
2025-05-01
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| Online Access: | https://doi.org/10.1038/s41598-025-03914-8 |
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| author | Yuqi Guo Xi Zhang Yanan Xing Yuxuan Liu Yixiao Liu Liuya Jia Lixin Xu Gaosheng He Jiting Ouyang Xu Yan |
| author_facet | Yuqi Guo Xi Zhang Yanan Xing Yuxuan Liu Yixiao Liu Liuya Jia Lixin Xu Gaosheng He Jiting Ouyang Xu Yan |
| author_sort | Yuqi Guo |
| collection | DOAJ |
| description | Abstract The interaction of atmospheric pressure plasma (APP) with the liquids has been fully studied, and multiple biomedical applications of APP-activated liquids have also been verified. However, there is still no direct evidence regarding the interaction between APP treatment and serum. In the present study, the rat serum was treated with the helium APP device. Compared with APP-treated saline, the elevation of reactive oxygen species (ROS) levels in serum was significantly smaller, implying that an antioxidant system existed in serum. The proteomics suggested that differently expressed proteins were predominantly enriched in the glutathione metabolism pathway. Our results showed that APP treatment significantly increased the serum GSH level and GSH/GSSG ratio, suggesting the enhanced antioxidant capacity of the APP-treated serum. Furthermore, the antioxidant enzymes in APP-treated serum were examined. As a result, both the glutathione peroxidase (GPX) protein level and enzyme activity were up-regulated according to the parallel reaction monitoring (PRM) analysis and GPX enzyme activity analysis. Meanwhile, the activities of glutathione reductase (GR) and superoxide dismutase (SOD) enzymes were also enhanced after APP treatment, while the catalase (CAT) activity decreased. Meanwhile, the total antioxidant capacity (T-AOC) also increased, as confirmed by the evidence of the superior ROS scavenging by APP-treated serum. Our data highlight that APP treatment can enhance the antioxidant capacity of the serum, which is closely related to the up-regulated GSH level, GSH/GSSG ratio and antioxidant enzymes within serum after APP treatment. |
| format | Article |
| id | doaj-art-ce42d4b9d896419cb166ad284bc3bbf2 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-ce42d4b9d896419cb166ad284bc3bbf22025-08-20T02:00:00ZengNature PortfolioScientific Reports2045-23222025-05-0115111110.1038/s41598-025-03914-8Atmospheric pressure plasma treatment increases the antioxidant capacity of the serum in vitroYuqi Guo0Xi Zhang1Yanan Xing2Yuxuan Liu3Yixiao Liu4Liuya Jia5Lixin Xu6Gaosheng He7Jiting Ouyang8Xu Yan9Department of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical UniversityDepartment of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical UniversitySchool of Physics, Beijing Institute of TechnologySchool of Physics, Beijing Institute of TechnologyDepartment of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical UniversityDepartment of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical UniversityDepartment of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical UniversitySchool of Physics, Beijing Institute of TechnologySchool of Physics, Beijing Institute of TechnologyDepartment of Pathophysiology, Beijing Neurosurgical Institute/Beijing Tiantan Hospital, Capital Medical UniversityAbstract The interaction of atmospheric pressure plasma (APP) with the liquids has been fully studied, and multiple biomedical applications of APP-activated liquids have also been verified. However, there is still no direct evidence regarding the interaction between APP treatment and serum. In the present study, the rat serum was treated with the helium APP device. Compared with APP-treated saline, the elevation of reactive oxygen species (ROS) levels in serum was significantly smaller, implying that an antioxidant system existed in serum. The proteomics suggested that differently expressed proteins were predominantly enriched in the glutathione metabolism pathway. Our results showed that APP treatment significantly increased the serum GSH level and GSH/GSSG ratio, suggesting the enhanced antioxidant capacity of the APP-treated serum. Furthermore, the antioxidant enzymes in APP-treated serum were examined. As a result, both the glutathione peroxidase (GPX) protein level and enzyme activity were up-regulated according to the parallel reaction monitoring (PRM) analysis and GPX enzyme activity analysis. Meanwhile, the activities of glutathione reductase (GR) and superoxide dismutase (SOD) enzymes were also enhanced after APP treatment, while the catalase (CAT) activity decreased. Meanwhile, the total antioxidant capacity (T-AOC) also increased, as confirmed by the evidence of the superior ROS scavenging by APP-treated serum. Our data highlight that APP treatment can enhance the antioxidant capacity of the serum, which is closely related to the up-regulated GSH level, GSH/GSSG ratio and antioxidant enzymes within serum after APP treatment.https://doi.org/10.1038/s41598-025-03914-8Atmospheric pressure plasmaReactive speciesSerumAntioxidant capacityPlasma medicine |
| spellingShingle | Yuqi Guo Xi Zhang Yanan Xing Yuxuan Liu Yixiao Liu Liuya Jia Lixin Xu Gaosheng He Jiting Ouyang Xu Yan Atmospheric pressure plasma treatment increases the antioxidant capacity of the serum in vitro Scientific Reports Atmospheric pressure plasma Reactive species Serum Antioxidant capacity Plasma medicine |
| title | Atmospheric pressure plasma treatment increases the antioxidant capacity of the serum in vitro |
| title_full | Atmospheric pressure plasma treatment increases the antioxidant capacity of the serum in vitro |
| title_fullStr | Atmospheric pressure plasma treatment increases the antioxidant capacity of the serum in vitro |
| title_full_unstemmed | Atmospheric pressure plasma treatment increases the antioxidant capacity of the serum in vitro |
| title_short | Atmospheric pressure plasma treatment increases the antioxidant capacity of the serum in vitro |
| title_sort | atmospheric pressure plasma treatment increases the antioxidant capacity of the serum in vitro |
| topic | Atmospheric pressure plasma Reactive species Serum Antioxidant capacity Plasma medicine |
| url | https://doi.org/10.1038/s41598-025-03914-8 |
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