Isolation of High-Molecular-Length DNA from Human Skin
An agarose plug method for isolating high-molecular-length DNA from mammalian tissues has been developed, including from those that are difficult, such as skin. It gives high yields of DNA that contain a minimum of single-strand breaks and is readily digested by restriction and other nucleases. The...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-09-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96213st03 |
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| _version_ | 1850151918011154432 |
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| author | P.V. Bennett R.W. Gange H. Hacham V.S. Hejmadi M. Moran S. Ray B.M. Sutherland |
| author_facet | P.V. Bennett R.W. Gange H. Hacham V.S. Hejmadi M. Moran S. Ray B.M. Sutherland |
| author_sort | P.V. Bennett |
| collection | DOAJ |
| description | An agarose plug method for isolating high-molecular-length DNA from mammalian tissues has been developed, including from those that are difficult, such as skin. It gives high yields of DNA that contain a minimum of single-strand breaks and is readily digested by restriction and other nucleases. The method requires only simple equipment and is readily adaptable to field or clinical studies. |
| format | Article |
| id | doaj-art-ce41b01ffb3c4bf498ccd4052dd63f79 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 1996-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-ce41b01ffb3c4bf498ccd4052dd63f792025-08-20T02:26:06ZengTaylor & Francis GroupBioTechniques0736-62051940-98181996-09-0121345846310.2144/96213st03Isolation of High-Molecular-Length DNA from Human SkinP.V. Bennett0R.W. Gange1H. Hacham2V.S. Hejmadi3M. Moran4S. Ray5B.M. Sutherland61Brookhaven National Laboratory, Upton, NY, USA1Brookhaven National Laboratory, Upton, NY, USA1Brookhaven National Laboratory, Upton, NY, USA1Brookhaven National Laboratory, Upton, NY, USA1Brookhaven National Laboratory, Upton, NY, USA1Brookhaven National Laboratory, Upton, NY, USA1Brookhaven National Laboratory, Upton, NY, USAAn agarose plug method for isolating high-molecular-length DNA from mammalian tissues has been developed, including from those that are difficult, such as skin. It gives high yields of DNA that contain a minimum of single-strand breaks and is readily digested by restriction and other nucleases. The method requires only simple equipment and is readily adaptable to field or clinical studies.https://www.future-science.com/doi/10.2144/96213st03 |
| spellingShingle | P.V. Bennett R.W. Gange H. Hacham V.S. Hejmadi M. Moran S. Ray B.M. Sutherland Isolation of High-Molecular-Length DNA from Human Skin BioTechniques |
| title | Isolation of High-Molecular-Length DNA from Human Skin |
| title_full | Isolation of High-Molecular-Length DNA from Human Skin |
| title_fullStr | Isolation of High-Molecular-Length DNA from Human Skin |
| title_full_unstemmed | Isolation of High-Molecular-Length DNA from Human Skin |
| title_short | Isolation of High-Molecular-Length DNA from Human Skin |
| title_sort | isolation of high molecular length dna from human skin |
| url | https://www.future-science.com/doi/10.2144/96213st03 |
| work_keys_str_mv | AT pvbennett isolationofhighmolecularlengthdnafromhumanskin AT rwgange isolationofhighmolecularlengthdnafromhumanskin AT hhacham isolationofhighmolecularlengthdnafromhumanskin AT vshejmadi isolationofhighmolecularlengthdnafromhumanskin AT mmoran isolationofhighmolecularlengthdnafromhumanskin AT sray isolationofhighmolecularlengthdnafromhumanskin AT bmsutherland isolationofhighmolecularlengthdnafromhumanskin |