Endophytic bacteria enhance the growth and salt tolerance of rice under saline conditions

Developing biostimulants from salt-tolerant plant growth-promoting (PGP) bacteria is an emerging strategy for sustainable agriculture in the context of increasing soil salinization. This study aimed to isolate endophytic bacteria (EB) capable of promoting rice seed germination and seedling growth at...

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Main Authors: Quang Trung DO, Anh The LUU, Tien Chuong NGO
Format: Article
Language:English
Published: University of Ljubljana Press (Založba Univerze v Ljubljani) 2023-04-01
Series:Acta Agriculturae Slovenica
Subjects:
Online Access:https://journals.uni-lj.si/aas/article/view/14398
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author Quang Trung DO
Anh The LUU
Tien Chuong NGO
author_facet Quang Trung DO
Anh The LUU
Tien Chuong NGO
author_sort Quang Trung DO
collection DOAJ
description Developing biostimulants from salt-tolerant plant growth-promoting (PGP) bacteria is an emerging strategy for sustainable agriculture in the context of increasing soil salinization. This study aimed to isolate endophytic bacteria (EB) capable of promoting rice seed germination and seedling growth at different NaCl concentrations. Nine salt-tolerant EB strains were isolated and two, ST.6 and ST.8, with the rice seed promoting effect 99.3 and 99.7 %, respectively, were selected and identified as Pantoea dispersa and Burkholderia cenocepacia, respectively. ST.6 showed a higher value of the activity of phosphatase (617 mg P ml-1), production of indole-3-acetic acid (19.7 µg IAA ml-1), the activity of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (13.5 µmol mg−1 protein h−1), and production of siderophore (76.3 %). Especially, rice seedlings inoculated with strain ST.6 showed a significant improvement in root length (58.95 %), shoot length (16.6 %), dry biomass (7.0 %), the content of chlorophyll (46.2 and 57.1 % for chlorophyll a and b, respectively), carotenoids (22.2%), and proline (19.0 %). A decrease in antioxidant enzyme activities was also observed in the rice seedlings inoculated with either ST.6 or ST.8 strain under salt stress. Furthermore, the salt stress condition enhanced the colonization of roots by both studied endophytic bacteria. More experiments should be done to develop endophytic bacteria ST.6 and ST.8 as efficient bio-inoculants.
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spelling doaj-art-ce3d4c528b7b4ad2aa5ea6428d190f432025-08-20T02:05:13ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412023-04-01119111110.14720/aas.2023.119.1.289920790Endophytic bacteria enhance the growth and salt tolerance of rice under saline conditionsQuang Trung DO0Anh The LUU1Tien Chuong NGO2Central Institute for Natural Resources and Environmental Studies, Vietnam National University Hanoi, Ha Noi, VietnamCentral Institute for Natural Resources and Environmental Studies, Vietnam National University Hanoi, Ha Noi, VietnamCentral Institute for Natural Resources and Environmental Studies, Vietnam National University Hanoi, Ha Noi, VietnamDeveloping biostimulants from salt-tolerant plant growth-promoting (PGP) bacteria is an emerging strategy for sustainable agriculture in the context of increasing soil salinization. This study aimed to isolate endophytic bacteria (EB) capable of promoting rice seed germination and seedling growth at different NaCl concentrations. Nine salt-tolerant EB strains were isolated and two, ST.6 and ST.8, with the rice seed promoting effect 99.3 and 99.7 %, respectively, were selected and identified as Pantoea dispersa and Burkholderia cenocepacia, respectively. ST.6 showed a higher value of the activity of phosphatase (617 mg P ml-1), production of indole-3-acetic acid (19.7 µg IAA ml-1), the activity of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (13.5 µmol mg−1 protein h−1), and production of siderophore (76.3 %). Especially, rice seedlings inoculated with strain ST.6 showed a significant improvement in root length (58.95 %), shoot length (16.6 %), dry biomass (7.0 %), the content of chlorophyll (46.2 and 57.1 % for chlorophyll a and b, respectively), carotenoids (22.2%), and proline (19.0 %). A decrease in antioxidant enzyme activities was also observed in the rice seedlings inoculated with either ST.6 or ST.8 strain under salt stress. Furthermore, the salt stress condition enhanced the colonization of roots by both studied endophytic bacteria. More experiments should be done to develop endophytic bacteria ST.6 and ST.8 as efficient bio-inoculants.https://journals.uni-lj.si/aas/article/view/14398endophytessalt stressplant growth-promoting bacteriaantioxidant enzymesseed germinationrice (oryza sativa)
spellingShingle Quang Trung DO
Anh The LUU
Tien Chuong NGO
Endophytic bacteria enhance the growth and salt tolerance of rice under saline conditions
Acta Agriculturae Slovenica
endophytes
salt stress
plant growth-promoting bacteria
antioxidant enzymes
seed germination
rice (oryza sativa)
title Endophytic bacteria enhance the growth and salt tolerance of rice under saline conditions
title_full Endophytic bacteria enhance the growth and salt tolerance of rice under saline conditions
title_fullStr Endophytic bacteria enhance the growth and salt tolerance of rice under saline conditions
title_full_unstemmed Endophytic bacteria enhance the growth and salt tolerance of rice under saline conditions
title_short Endophytic bacteria enhance the growth and salt tolerance of rice under saline conditions
title_sort endophytic bacteria enhance the growth and salt tolerance of rice under saline conditions
topic endophytes
salt stress
plant growth-promoting bacteria
antioxidant enzymes
seed germination
rice (oryza sativa)
url https://journals.uni-lj.si/aas/article/view/14398
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AT tienchuongngo endophyticbacteriaenhancethegrowthandsalttoleranceofriceundersalineconditions