Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds
Objective:Iris species are widely used in pharmaceutical and cosmetic applications owing to their high content of bioactive compounds with anti-inflammatory and antimicrobial properties. This study aimed to investigate the potential antibacterial effect of crude extracts (aqueous, 50% and 80% ethano...
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Istanbul University Press
2024-12-01
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| Series: | European Journal of Biology |
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| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/5EFF74B3CBA94FC39EFE9C52A21D335C |
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| author | Belmina Šarić Medić Anesa Jerković Mujkić Berina Cubara Adaleta Durmić Pašić Jasna Hanjalić Kurtović Kasim Bajrovic Elma Omeragić Mirza Dedić Faruk Bogunić Lejla Pojskić |
| author_facet | Belmina Šarić Medić Anesa Jerković Mujkić Berina Cubara Adaleta Durmić Pašić Jasna Hanjalić Kurtović Kasim Bajrovic Elma Omeragić Mirza Dedić Faruk Bogunić Lejla Pojskić |
| author_sort | Belmina Šarić Medić |
| collection | DOAJ |
| description | Objective:Iris species are widely used in pharmaceutical and cosmetic applications owing to their high content of bioactive compounds with anti-inflammatory and antimicrobial properties. This study aimed to investigate the potential antibacterial effect of crude extracts (aqueous, 50% and 80% ethanol) of three Iris species (I. pumila, while I. reichenbachii and I. illyrica are endemic) from Bosnia and Herzegovina against the multiresistant bacterial strain methicillin-resistant Staphylococcus aureus subsp. aureus ATCC 33591 (MRSA strain).Materials and Methods: The antimicrobial compounds in the crude extracts were identified using High-performance liquid chromatography (HPLC), and their effects on the MRSA strain were tested using agar well diffusion and broth microdilution method. The binding affinities were analysed using molecular docking simulations.Results: We identified bioactive targeted compounds in these extracts, mainly flavonoids named isorhamnetin, hesperidin, quercetin, fisetin, genistein, and kaempferol. Antibacterial assays showed that extracts of all three Iris species inhibited MRSA. The binding affinity analysis showed that isorhamnetin and hesperidin had the highest affinity scores, stronger (isorhamnetin) or the same (hesperidin) as the positive control ceftobiprole.Conclusion: Thisin vitro and in silico study showed that Iris species represent a valuable source of bioactive compounds that can be used against multidrug-resistant strains such as MRSA. The potential use of these agents in multiple drugs is warranted, and further evaluation for human application is needed. |
| format | Article |
| id | doaj-art-ce315f6ea4054af18bf86c7abf397d1a |
| institution | OA Journals |
| issn | 2618-6144 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | European Journal of Biology |
| spelling | doaj-art-ce315f6ea4054af18bf86c7abf397d1a2025-08-20T02:11:21ZengIstanbul University PressEuropean Journal of Biology2618-61442024-12-0183218218810.26650/EurJBiol.2024.1480514123456Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial CompoundsBelmina Šarić Medić0https://orcid.org/0000-0001-8593-4678Anesa Jerković Mujkić1https://orcid.org/0000-0002-7769-4181Berina Cubara2https://orcid.org/0009-0007-1965-3297Adaleta Durmić Pašić3https://orcid.org/0000-0002-5022-6185Jasna Hanjalić Kurtović4https://orcid.org/0000-0002-8693-7886Kasim Bajrovic5Elma Omeragić6https://orcid.org/0000-0002-5996-4926Mirza Dedić7https://orcid.org/0000-0002-4951-7516Faruk Bogunić8https://orcid.org/0000-0002-7636-3559Lejla Pojskić9https://orcid.org/0000-0003-2260-318XUniversity of Sarajevo, Sarajevo, Bosnia-And-HerzegovinaUniversity of Sarajevo, Sarajevo, Bosnia-And-HerzegovinaUniversity of Sarajevo, Sarajevo, Bosnia-And-HerzegovinaUniversity of Sarajevo, Sarajevo, Bosnia-And-HerzegovinaUniversity of Sarajevo, Sarajevo, Bosnia-And-HerzegovinaUniversity of Sarajevo, Sarajevo, Bosnia-And-HerzegovinaUniversity of Sarajevo, Sarajevo, Bosnia-And-HerzegovinaUniversity of Sarajevo, Sarajevo, Bosnia-And-HerzegovinaUniversity of Sarajevo, Sarajevo, Bosnia-And-HerzegovinaUniversity of Sarajevo, Sarajevo, Bosnia-And-HerzegovinaObjective:Iris species are widely used in pharmaceutical and cosmetic applications owing to their high content of bioactive compounds with anti-inflammatory and antimicrobial properties. This study aimed to investigate the potential antibacterial effect of crude extracts (aqueous, 50% and 80% ethanol) of three Iris species (I. pumila, while I. reichenbachii and I. illyrica are endemic) from Bosnia and Herzegovina against the multiresistant bacterial strain methicillin-resistant Staphylococcus aureus subsp. aureus ATCC 33591 (MRSA strain).Materials and Methods: The antimicrobial compounds in the crude extracts were identified using High-performance liquid chromatography (HPLC), and their effects on the MRSA strain were tested using agar well diffusion and broth microdilution method. The binding affinities were analysed using molecular docking simulations.Results: We identified bioactive targeted compounds in these extracts, mainly flavonoids named isorhamnetin, hesperidin, quercetin, fisetin, genistein, and kaempferol. Antibacterial assays showed that extracts of all three Iris species inhibited MRSA. The binding affinity analysis showed that isorhamnetin and hesperidin had the highest affinity scores, stronger (isorhamnetin) or the same (hesperidin) as the positive control ceftobiprole.Conclusion: Thisin vitro and in silico study showed that Iris species represent a valuable source of bioactive compounds that can be used against multidrug-resistant strains such as MRSA. The potential use of these agents in multiple drugs is warranted, and further evaluation for human application is needed.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/5EFF74B3CBA94FC39EFE9C52A21D335Cplant bioactive compoundsmethicillin-resistant staphylococcus aureusmolecular dockingminimum inhibitory concentration |
| spellingShingle | Belmina Šarić Medić Anesa Jerković Mujkić Berina Cubara Adaleta Durmić Pašić Jasna Hanjalić Kurtović Kasim Bajrovic Elma Omeragić Mirza Dedić Faruk Bogunić Lejla Pojskić Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds European Journal of Biology plant bioactive compounds methicillin-resistant staphylococcus aureus molecular docking minimum inhibitory concentration |
| title | Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds |
| title_full | Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds |
| title_fullStr | Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds |
| title_full_unstemmed | Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds |
| title_short | Crude Extracts of Three Iris Species as Sources of MRSA Antimicrobial Compounds |
| title_sort | crude extracts of three iris species as sources of mrsa antimicrobial compounds |
| topic | plant bioactive compounds methicillin-resistant staphylococcus aureus molecular docking minimum inhibitory concentration |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/5EFF74B3CBA94FC39EFE9C52A21D335C |
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