Antitumor effect of mifepristone on human endometrial stromal cell line
Background/Aim. The main cause for development of endometrial hyperplasia is unopposed effect of estrogen on endometrial cells. The aim of our study was to investigate and compare cytotoxic and apoptotic effects of mifepristone on human endometrial stromal cell line for the first time. Both percenta...
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2021-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900104L.pdf |
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| author | Luković Jovan Milosavljević Zoran Zečević-Luković Tanja Mitrović Marina Anđelković Marija Zelen Ivanka Stanojević-Pirković Marijana Nikolić Ivana |
| author_facet | Luković Jovan Milosavljević Zoran Zečević-Luković Tanja Mitrović Marina Anđelković Marija Zelen Ivanka Stanojević-Pirković Marijana Nikolić Ivana |
| author_sort | Luković Jovan |
| collection | DOAJ |
| description | Background/Aim. The main cause for development of endometrial hyperplasia is unopposed effect of estrogen on endometrial cells. The aim of our study was to investigate and compare cytotoxic and apoptotic effects of mifepristone on human endometrial stromal cell line for the first time. Both percentage of cytotoxic and apoptotic cells were determined after 24 h treatment with different doses of mifepristone. Methods. The percentage of cytotoxic cell was evaluated by viability assay while the percentage of apoptotic cells was determined using flow cytometry. Determination of apoptotic effects was confirmed using immunofluorescence method determining expression and localization of active Bax and Bcl-2 proteins. Results. Our results indicated that mifepristone induced cytotoxic and apoptotic effect on human endometrial stromal cell line (ThESC) through changes in expression level of Bcl-2 and active Bax proteins. Conclusion. Cytotoxic and pro-apoptotic effects of mifepristone on human endometrial stromal cell line in vitro was investigated in this study for the first time. It is crucial to point out that mifepristone expressed both cytotoxic and pro-apoptotic effect on ThESC cell line. Our results may contribute to determination of localization and expression level of the crucial proteins involved in apoptosis in ThESC cell line after the treatment with the lowest cytotoxic doses of mifepristone. |
| format | Article |
| id | doaj-art-ce2d33c460c44066a6097b663f89e72c |
| institution | OA Journals |
| issn | 0042-8450 2406-0720 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-ce2d33c460c44066a6097b663f89e72c2025-08-20T02:04:27ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202021-01-0178661562010.2298/VSP190226104L0042-84501900104LAntitumor effect of mifepristone on human endometrial stromal cell lineLuković Jovan0Milosavljević Zoran1https://orcid.org/0000-0002-6078-0665Zečević-Luković Tanja2https://orcid.org/0000-0003-3272-5420Mitrović Marina3Anđelković Marija4Zelen Ivanka5Stanojević-Pirković Marijana6Nikolić Ivana7University of Kragujevac, Faculty of Medical Sciences, Department of Biochemistry, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Histology and Embryology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Physical Medicine and Rehabilitation, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Biochemistry, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Biochemistry, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Biochemistry, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Biochemistry, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Biochemistry, Kragujevac, SerbiaBackground/Aim. The main cause for development of endometrial hyperplasia is unopposed effect of estrogen on endometrial cells. The aim of our study was to investigate and compare cytotoxic and apoptotic effects of mifepristone on human endometrial stromal cell line for the first time. Both percentage of cytotoxic and apoptotic cells were determined after 24 h treatment with different doses of mifepristone. Methods. The percentage of cytotoxic cell was evaluated by viability assay while the percentage of apoptotic cells was determined using flow cytometry. Determination of apoptotic effects was confirmed using immunofluorescence method determining expression and localization of active Bax and Bcl-2 proteins. Results. Our results indicated that mifepristone induced cytotoxic and apoptotic effect on human endometrial stromal cell line (ThESC) through changes in expression level of Bcl-2 and active Bax proteins. Conclusion. Cytotoxic and pro-apoptotic effects of mifepristone on human endometrial stromal cell line in vitro was investigated in this study for the first time. It is crucial to point out that mifepristone expressed both cytotoxic and pro-apoptotic effect on ThESC cell line. Our results may contribute to determination of localization and expression level of the crucial proteins involved in apoptosis in ThESC cell line after the treatment with the lowest cytotoxic doses of mifepristone.http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900104L.pdfendometrial hyperplasiamifepristoneapoptosiscell deathcarcinoma |
| spellingShingle | Luković Jovan Milosavljević Zoran Zečević-Luković Tanja Mitrović Marina Anđelković Marija Zelen Ivanka Stanojević-Pirković Marijana Nikolić Ivana Antitumor effect of mifepristone on human endometrial stromal cell line Vojnosanitetski Pregled endometrial hyperplasia mifepristone apoptosis cell death carcinoma |
| title | Antitumor effect of mifepristone on human endometrial stromal cell line |
| title_full | Antitumor effect of mifepristone on human endometrial stromal cell line |
| title_fullStr | Antitumor effect of mifepristone on human endometrial stromal cell line |
| title_full_unstemmed | Antitumor effect of mifepristone on human endometrial stromal cell line |
| title_short | Antitumor effect of mifepristone on human endometrial stromal cell line |
| title_sort | antitumor effect of mifepristone on human endometrial stromal cell line |
| topic | endometrial hyperplasia mifepristone apoptosis cell death carcinoma |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2021/0042-84501900104L.pdf |
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