Protocol for engineering bone organoids from mesenchymal stem cells
Bone organoids are emerging as powerful tools for studying bone development and related diseases. However, the simplified design of current methods somewhat limits their application potential, as these methods produce single-tissue organoids that fail to replicate the bone microarchitecture or achie...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2025-03-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X24005012 |
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| author | Jian Wang Dongyang Zhou Ruiyang Li Shihao Sheng Guangfeng Li Yue Sun Peng Wang Yulin Mo Han Liu Xiao Chen Zhen Geng Qin Zhang Yingying Jing Long Bai Ke Xu Jiacan Su |
| author_facet | Jian Wang Dongyang Zhou Ruiyang Li Shihao Sheng Guangfeng Li Yue Sun Peng Wang Yulin Mo Han Liu Xiao Chen Zhen Geng Qin Zhang Yingying Jing Long Bai Ke Xu Jiacan Su |
| author_sort | Jian Wang |
| collection | DOAJ |
| description | Bone organoids are emerging as powerful tools for studying bone development and related diseases. However, the simplified design of current methods somewhat limits their application potential, as these methods produce single-tissue organoids that fail to replicate the bone microarchitecture or achieve effective mineralization. To address this issue, we propose a three-dimensional (3D) construction strategy for generating mineralized bone structures using bone marrow-derived mesenchymal stem cells (BMSCs). By mixing BMSCs with hydrogel to create a bone matrix-mimicking bioink and employing projection-based light-curing 3D printing technology, we constructed 3D-printed structures, which were then implanted subcutaneously into nude mice, away from the native bone microenvironment. Even without external stimulation, these implants spontaneously formed mineralized bone domains. With long-term culture, these structures gradually matured into fully differentiated bone tissue, completing both mineralization and vascularization. This in vivo bone organoid model offers a novel platform for studying bone development, exploring congenital diseases, testing drugs, and developing therapeutic applications. |
| format | Article |
| id | doaj-art-ce2993374f9c4541913d11b5bfa3822a |
| institution | Kabale University |
| issn | 2452-199X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj-art-ce2993374f9c4541913d11b5bfa3822a2025-01-26T05:04:22ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2025-03-0145388400Protocol for engineering bone organoids from mesenchymal stem cellsJian Wang0Dongyang Zhou1Ruiyang Li2Shihao Sheng3Guangfeng Li4Yue Sun5Peng Wang6Yulin Mo7Han Liu8Xiao Chen9Zhen Geng10Qin Zhang11Yingying Jing12Long Bai13Ke Xu14Jiacan Su15Department of Orthopedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Trauma Orthopedics Center, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Institute of Musculoskeletal Injury and Translational Medicine of Organoids, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China; School of Medicine, Shanghai University, Shanghai, 200444, China; National Center for Translational Medicine SHU Branch, Shanghai University, Shanghai, 200444, ChinaInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China; National Center for Translational Medicine SHU Branch, Shanghai University, Shanghai, 200444, ChinaDepartment of Orthopedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Trauma Orthopedics Center, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Institute of Musculoskeletal Injury and Translational Medicine of Organoids, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, ChinaDepartment of Orthopedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Trauma Orthopedics Center, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Institute of Musculoskeletal Injury and Translational Medicine of Organoids, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, ChinaInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China; School of Medicine, Shanghai University, Shanghai, 200444, China; National Center for Translational Medicine SHU Branch, Shanghai University, Shanghai, 200444, China; Department of Orthopedics, Shanghai Zhongye Hospital, Shanghai, 200941, ChinaInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China; School of Medicine, Shanghai University, Shanghai, 200444, China; National Center for Translational Medicine SHU Branch, Shanghai University, Shanghai, 200444, ChinaDepartment of Orthopedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Trauma Orthopedics Center, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Institute of Musculoskeletal Injury and Translational Medicine of Organoids, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, ChinaInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China; School of Medicine, Shanghai University, Shanghai, 200444, China; National Center for Translational Medicine SHU Branch, Shanghai University, Shanghai, 200444, ChinaInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China; National Center for Translational Medicine SHU Branch, Shanghai University, Shanghai, 200444, ChinaDepartment of Orthopedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Trauma Orthopedics Center, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Institute of Musculoskeletal Injury and Translational Medicine of Organoids, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China; National Center for Translational Medicine SHU Branch, Shanghai University, Shanghai, 200444, ChinaInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China; National Center for Translational Medicine SHU Branch, Shanghai University, Shanghai, 200444, ChinaInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China; National Center for Translational Medicine SHU Branch, Shanghai University, Shanghai, 200444, ChinaInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China; National Center for Translational Medicine SHU Branch, Shanghai University, Shanghai, 200444, China; Corresponding author. Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China.Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China; National Center for Translational Medicine SHU Branch, Shanghai University, Shanghai, 200444, China; Corresponding author. Institute of Translational Medicine, Shanghai University, Shanghai, 200444, ChinaInstitute of Translational Medicine, Shanghai University, Shanghai, 200444, China; National Center for Translational Medicine SHU Branch, Shanghai University, Shanghai, 200444, China; Corresponding author. Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China.Department of Orthopedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Trauma Orthopedics Center, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Institute of Musculoskeletal Injury and Translational Medicine of Organoids, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China; Institute of Translational Medicine, Shanghai University, Shanghai, 200444, China; National Center for Translational Medicine SHU Branch, Shanghai University, Shanghai, 200444, China; Corresponding author. Department of Orthopedics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China.Bone organoids are emerging as powerful tools for studying bone development and related diseases. However, the simplified design of current methods somewhat limits their application potential, as these methods produce single-tissue organoids that fail to replicate the bone microarchitecture or achieve effective mineralization. To address this issue, we propose a three-dimensional (3D) construction strategy for generating mineralized bone structures using bone marrow-derived mesenchymal stem cells (BMSCs). By mixing BMSCs with hydrogel to create a bone matrix-mimicking bioink and employing projection-based light-curing 3D printing technology, we constructed 3D-printed structures, which were then implanted subcutaneously into nude mice, away from the native bone microenvironment. Even without external stimulation, these implants spontaneously formed mineralized bone domains. With long-term culture, these structures gradually matured into fully differentiated bone tissue, completing both mineralization and vascularization. This in vivo bone organoid model offers a novel platform for studying bone development, exploring congenital diseases, testing drugs, and developing therapeutic applications.http://www.sciencedirect.com/science/article/pii/S2452199X24005012Bone organoids3D bioprintingBioinkMineralizationVascularization |
| spellingShingle | Jian Wang Dongyang Zhou Ruiyang Li Shihao Sheng Guangfeng Li Yue Sun Peng Wang Yulin Mo Han Liu Xiao Chen Zhen Geng Qin Zhang Yingying Jing Long Bai Ke Xu Jiacan Su Protocol for engineering bone organoids from mesenchymal stem cells Bioactive Materials Bone organoids 3D bioprinting Bioink Mineralization Vascularization |
| title | Protocol for engineering bone organoids from mesenchymal stem cells |
| title_full | Protocol for engineering bone organoids from mesenchymal stem cells |
| title_fullStr | Protocol for engineering bone organoids from mesenchymal stem cells |
| title_full_unstemmed | Protocol for engineering bone organoids from mesenchymal stem cells |
| title_short | Protocol for engineering bone organoids from mesenchymal stem cells |
| title_sort | protocol for engineering bone organoids from mesenchymal stem cells |
| topic | Bone organoids 3D bioprinting Bioink Mineralization Vascularization |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X24005012 |
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