Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease
Porphyromonas gingivalis and Treponema denticola are periodontal pathogens that express virulence factors associated with the pathogenesis of periodontitis. In this paper we tested the hypothesis that P. gingivalis and T. denticola are synergistic in terms of virulence; using a model of mixed microb...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Interdisciplinary Perspectives on Infectious Diseases |
| Online Access: | http://dx.doi.org/10.1155/2010/605125 |
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| author | Raj K. Verma Sunethra Rajapakse Archana Meka Clayton Hamrick Sheela Pola Indraneel Bhattacharyya Madhu Nair Shannon M. Wallet Ikramuddin Aukhil Lakshmyya Kesavalu |
| author_facet | Raj K. Verma Sunethra Rajapakse Archana Meka Clayton Hamrick Sheela Pola Indraneel Bhattacharyya Madhu Nair Shannon M. Wallet Ikramuddin Aukhil Lakshmyya Kesavalu |
| author_sort | Raj K. Verma |
| collection | DOAJ |
| description | Porphyromonas gingivalis and Treponema denticola are periodontal pathogens that express virulence factors associated with the pathogenesis of periodontitis. In this paper we tested the hypothesis that P. gingivalis and T. denticola are synergistic in terms of virulence; using a model of mixed microbial infection in rats. Groups of rats were orally infected with either P. gingivalis or T. denticola or mixed microbial infections for 7 and 12 weeks. P. gingivalis genomic DNA was detected more frequently by PCR than T. denticola. Both bacteria induced significantly high IgG, IgG2b, IgG1, IgG2a antibody levels indicating a stimulation of Th1 and Th2 immune response. Radiographic and morphometric measurements demonstrated that rats infected with the mixed infection exhibited significantly more alveolar bone loss than shaminfected control rats. Histology revealed apical migration of junctional epithelium, rete ridge elongation, and crestal alveolar bone resorption; resembling periodontal disease lesion. These results showed that P. gingivalis and T. denticola exhibit no synergistic virulence in a rat model of periodontal disease. |
| format | Article |
| id | doaj-art-ce1de2fa7f984b73a06d8d8798135fdf |
| institution | DOAJ |
| issn | 1687-708X 1687-7098 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Interdisciplinary Perspectives on Infectious Diseases |
| spelling | doaj-art-ce1de2fa7f984b73a06d8d8798135fdf2025-08-20T03:19:41ZengWileyInterdisciplinary Perspectives on Infectious Diseases1687-708X1687-70982010-01-01201010.1155/2010/605125605125Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal DiseaseRaj K. Verma0Sunethra Rajapakse1Archana Meka2Clayton Hamrick3Sheela Pola4Indraneel Bhattacharyya5Madhu Nair6Shannon M. Wallet7Ikramuddin Aukhil8Lakshmyya Kesavalu9Department of Periodontology, College of Dentistry, University of Florida, Gainesville, FL 32610, USADepartment of Periodontology, College of Dentistry, University of Florida, Gainesville, FL 32610, USADepartment of Periodontology, College of Dentistry, University of Florida, Gainesville, FL 32610, USADepartment of Periodontology, College of Dentistry, University of Florida, Gainesville, FL 32610, USADepartment of Periodontology, College of Dentistry, University of Florida, Gainesville, FL 32610, USADepartment of Oral & Maxillofacial Diagnostic Sciences, College of Dentistry, University of Florida, Gainesville, FL 32610, USADepartment of Oral & Maxillofacial Diagnostic Sciences, College of Dentistry, University of Florida, Gainesville, FL 32610, USADepartment of Periodontology, College of Dentistry, University of Florida, Gainesville, FL 32610, USADepartment of Periodontology, College of Dentistry, University of Florida, Gainesville, FL 32610, USADepartment of Periodontology, College of Dentistry, University of Florida, Gainesville, FL 32610, USAPorphyromonas gingivalis and Treponema denticola are periodontal pathogens that express virulence factors associated with the pathogenesis of periodontitis. In this paper we tested the hypothesis that P. gingivalis and T. denticola are synergistic in terms of virulence; using a model of mixed microbial infection in rats. Groups of rats were orally infected with either P. gingivalis or T. denticola or mixed microbial infections for 7 and 12 weeks. P. gingivalis genomic DNA was detected more frequently by PCR than T. denticola. Both bacteria induced significantly high IgG, IgG2b, IgG1, IgG2a antibody levels indicating a stimulation of Th1 and Th2 immune response. Radiographic and morphometric measurements demonstrated that rats infected with the mixed infection exhibited significantly more alveolar bone loss than shaminfected control rats. Histology revealed apical migration of junctional epithelium, rete ridge elongation, and crestal alveolar bone resorption; resembling periodontal disease lesion. These results showed that P. gingivalis and T. denticola exhibit no synergistic virulence in a rat model of periodontal disease.http://dx.doi.org/10.1155/2010/605125 |
| spellingShingle | Raj K. Verma Sunethra Rajapakse Archana Meka Clayton Hamrick Sheela Pola Indraneel Bhattacharyya Madhu Nair Shannon M. Wallet Ikramuddin Aukhil Lakshmyya Kesavalu Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease Interdisciplinary Perspectives on Infectious Diseases |
| title | Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease |
| title_full | Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease |
| title_fullStr | Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease |
| title_full_unstemmed | Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease |
| title_short | Porphyromonas gingivalis and Treponema denticola Mixed Microbial Infection in a Rat Model of Periodontal Disease |
| title_sort | porphyromonas gingivalis and treponema denticola mixed microbial infection in a rat model of periodontal disease |
| url | http://dx.doi.org/10.1155/2010/605125 |
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