Rapid preparation of bioactive composites for transforaminal lumbar interbody fusion
Abstract The current stem cell tissue engineering always requires in vitro cell culture. To solve this problem, the bone mesenchymal stem cells (MSCs) screen-enrich-combine circulating system (SECCS) was created to rapidly enrich stem cells and combined with β-tricalcium phosphate (β-TCP) to immedia...
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Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-86776-4 |
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| author | Baozhi Ding Xin Wang Changqing Zhao Jie Zhao |
| author_facet | Baozhi Ding Xin Wang Changqing Zhao Jie Zhao |
| author_sort | Baozhi Ding |
| collection | DOAJ |
| description | Abstract The current stem cell tissue engineering always requires in vitro cell culture. To solve this problem, the bone mesenchymal stem cells (MSCs) screen-enrich-combine circulating system (SECCS) was created to rapidly enrich stem cells and combined with β-tricalcium phosphate (β-TCP) to immediately produce bioactive MSCs/β-TCP composites. 37 patients who underwent transforaminal lumbar interbody fusion (TLIF) surgery were included in this study and randomly divided into two groups. One group uses laminal bone grafts (LBG) for intervertebral fusion, and another group uses MSCs/β-TCP composites. The new technique could quickly and selectively enrich stem cells from the bone marrow and simultaneously implant them into the intervertebral space during operation. The cells eluted from the β-tricalcium phosphate were confirmed to be stem cells based on their characteristics. Higher early fusion rates, similar intervertebral height decreases, and functional improvement tendencies were observed in the SECCS group when compared to the LBG group. The bioactive scaffold prepared by SECCS showed better osteogenic efficiency at the early stage of intervertebral fusion compared to autologous LBG, which provided a new bone scaffold substitute for autologous bone. |
| format | Article |
| id | doaj-art-ce0e6000181f4bd6be8d9b6586c0b616 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-ce0e6000181f4bd6be8d9b6586c0b6162025-02-09T12:28:40ZengNature PortfolioScientific Reports2045-23222025-02-0115111010.1038/s41598-025-86776-4Rapid preparation of bioactive composites for transforaminal lumbar interbody fusionBaozhi Ding0Xin Wang1Changqing Zhao2Jie Zhao3Shanghai Key Laboratory of Orthopaedic ImplantsShanghai Key Laboratory of Orthopaedic ImplantsShanghai Key Laboratory of Orthopaedic ImplantsShanghai Key Laboratory of Orthopaedic ImplantsAbstract The current stem cell tissue engineering always requires in vitro cell culture. To solve this problem, the bone mesenchymal stem cells (MSCs) screen-enrich-combine circulating system (SECCS) was created to rapidly enrich stem cells and combined with β-tricalcium phosphate (β-TCP) to immediately produce bioactive MSCs/β-TCP composites. 37 patients who underwent transforaminal lumbar interbody fusion (TLIF) surgery were included in this study and randomly divided into two groups. One group uses laminal bone grafts (LBG) for intervertebral fusion, and another group uses MSCs/β-TCP composites. The new technique could quickly and selectively enrich stem cells from the bone marrow and simultaneously implant them into the intervertebral space during operation. The cells eluted from the β-tricalcium phosphate were confirmed to be stem cells based on their characteristics. Higher early fusion rates, similar intervertebral height decreases, and functional improvement tendencies were observed in the SECCS group when compared to the LBG group. The bioactive scaffold prepared by SECCS showed better osteogenic efficiency at the early stage of intervertebral fusion compared to autologous LBG, which provided a new bone scaffold substitute for autologous bone.https://doi.org/10.1038/s41598-025-86776-4Bone mesenchymal stem cellsEnrichmentΒ-tricalcium phosphateTransforaminal lumbar interbody fusion |
| spellingShingle | Baozhi Ding Xin Wang Changqing Zhao Jie Zhao Rapid preparation of bioactive composites for transforaminal lumbar interbody fusion Scientific Reports Bone mesenchymal stem cells Enrichment Β-tricalcium phosphate Transforaminal lumbar interbody fusion |
| title | Rapid preparation of bioactive composites for transforaminal lumbar interbody fusion |
| title_full | Rapid preparation of bioactive composites for transforaminal lumbar interbody fusion |
| title_fullStr | Rapid preparation of bioactive composites for transforaminal lumbar interbody fusion |
| title_full_unstemmed | Rapid preparation of bioactive composites for transforaminal lumbar interbody fusion |
| title_short | Rapid preparation of bioactive composites for transforaminal lumbar interbody fusion |
| title_sort | rapid preparation of bioactive composites for transforaminal lumbar interbody fusion |
| topic | Bone mesenchymal stem cells Enrichment Β-tricalcium phosphate Transforaminal lumbar interbody fusion |
| url | https://doi.org/10.1038/s41598-025-86776-4 |
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