Microscopical Observation and Transcriptome Analysis Reveal the Effects of High‐Altitude Ecosystem in the Qualities of Different Genetic Varieties Brassica napus Resources
ABSTRACT Improving the biomass and nutritional quality of Brassica napus is important for its breeding and resource conservation, but there are few studies on the effects of high altitude on its biomass and quality. In this study, 27 varieties of Brassica napus were cultivated both at 1600 m and 150...
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| Format: | Article |
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Wiley
2024-11-01
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| Series: | Ecology and Evolution |
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| Online Access: | https://doi.org/10.1002/ece3.70616 |
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| author | Zongji Zhang Xionghua Li Ri Ming Yingying Lu Qinwen Lin Yafei Yang Jialin Liao Yunjuan Li Lingli Mao Yang Huang Li Zhong Yu Liang |
| author_facet | Zongji Zhang Xionghua Li Ri Ming Yingying Lu Qinwen Lin Yafei Yang Jialin Liao Yunjuan Li Lingli Mao Yang Huang Li Zhong Yu Liang |
| author_sort | Zongji Zhang |
| collection | DOAJ |
| description | ABSTRACT Improving the biomass and nutritional quality of Brassica napus is important for its breeding and resource conservation, but there are few studies on the effects of high altitude on its biomass and quality. In this study, 27 varieties of Brassica napus were cultivated both at 1600 m and 150 m altitudes to investigate the effect of different altitudes in the biomass and quality traits of B. napus. At high altitude, all B. napus varieties exhibited decreased picking period, reduced fresh and dry weights, diminished stem length and diameter, as well as lowered nitrogen (N), phosphorus (P), and kalium (K) contents. The soluble sugar and cellulose contents of some varieties decreased, while ascorbic acid and protein increased by 74% and 85%, respectively. Furthermore, histology microscopical observation showed cell size increased and cell density decreased in the shoot apical meristem (SAM) after bolting and at high altitude, compared to those cells before bolting and at low altitude. Transcriptome analysis showed that sucrose phosphate synthase (SPS), sucrose synthase (SUS), fructose‐1,6‐bisphosphate aldolase (FBA1), and alpha‐galactosidase (AGAL2) genes were significantly up‐regulated during the after‐bolting period at different altitude. This study will be helpful to further understand the influence of high altitude ecosystem on biomass and quality for Brassica napus resource and evolution. |
| format | Article |
| id | doaj-art-ce09a45d1b2b46dcb60424ba0922e6fe |
| institution | OA Journals |
| issn | 2045-7758 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj-art-ce09a45d1b2b46dcb60424ba0922e6fe2025-08-20T02:34:35ZengWileyEcology and Evolution2045-77582024-11-011411n/an/a10.1002/ece3.70616Microscopical Observation and Transcriptome Analysis Reveal the Effects of High‐Altitude Ecosystem in the Qualities of Different Genetic Varieties Brassica napus ResourcesZongji Zhang0Xionghua Li1Ri Ming2Yingying Lu3Qinwen Lin4Yafei Yang5Jialin Liao6Yunjuan Li7Lingli Mao8Yang Huang9Li Zhong10Yu Liang11Guilin Branch of Guangxi Academy of Agricultural Science/Guilin Research Centre of Agricultural Sciences Guilin ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin, College of Life Science Guangxi Normal University Guilin ChinaGuilin Branch of Guangxi Academy of Agricultural Science/Guilin Research Centre of Agricultural Sciences Guilin ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin, College of Life Science Guangxi Normal University Guilin ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin, College of Life Science Guangxi Normal University Guilin ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin, College of Life Science Guangxi Normal University Guilin ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin, College of Life Science Guangxi Normal University Guilin ChinaGuilin Branch of Guangxi Academy of Agricultural Science/Guilin Research Centre of Agricultural Sciences Guilin ChinaGuilin Branch of Guangxi Academy of Agricultural Science/Guilin Research Centre of Agricultural Sciences Guilin ChinaSchool of Mechanical and Electrical Engineering Guilin University of Electronic Technology Guilin ChinaGuilin Branch of Guangxi Academy of Agricultural Science/Guilin Research Centre of Agricultural Sciences Guilin ChinaKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin, College of Life Science Guangxi Normal University Guilin ChinaABSTRACT Improving the biomass and nutritional quality of Brassica napus is important for its breeding and resource conservation, but there are few studies on the effects of high altitude on its biomass and quality. In this study, 27 varieties of Brassica napus were cultivated both at 1600 m and 150 m altitudes to investigate the effect of different altitudes in the biomass and quality traits of B. napus. At high altitude, all B. napus varieties exhibited decreased picking period, reduced fresh and dry weights, diminished stem length and diameter, as well as lowered nitrogen (N), phosphorus (P), and kalium (K) contents. The soluble sugar and cellulose contents of some varieties decreased, while ascorbic acid and protein increased by 74% and 85%, respectively. Furthermore, histology microscopical observation showed cell size increased and cell density decreased in the shoot apical meristem (SAM) after bolting and at high altitude, compared to those cells before bolting and at low altitude. Transcriptome analysis showed that sucrose phosphate synthase (SPS), sucrose synthase (SUS), fructose‐1,6‐bisphosphate aldolase (FBA1), and alpha‐galactosidase (AGAL2) genes were significantly up‐regulated during the after‐bolting period at different altitude. This study will be helpful to further understand the influence of high altitude ecosystem on biomass and quality for Brassica napus resource and evolution.https://doi.org/10.1002/ece3.70616altitudebiomassboltingBrassica napusnutritional qualitypicking period |
| spellingShingle | Zongji Zhang Xionghua Li Ri Ming Yingying Lu Qinwen Lin Yafei Yang Jialin Liao Yunjuan Li Lingli Mao Yang Huang Li Zhong Yu Liang Microscopical Observation and Transcriptome Analysis Reveal the Effects of High‐Altitude Ecosystem in the Qualities of Different Genetic Varieties Brassica napus Resources Ecology and Evolution altitude biomass bolting Brassica napus nutritional quality picking period |
| title | Microscopical Observation and Transcriptome Analysis Reveal the Effects of High‐Altitude Ecosystem in the Qualities of Different Genetic Varieties Brassica napus Resources |
| title_full | Microscopical Observation and Transcriptome Analysis Reveal the Effects of High‐Altitude Ecosystem in the Qualities of Different Genetic Varieties Brassica napus Resources |
| title_fullStr | Microscopical Observation and Transcriptome Analysis Reveal the Effects of High‐Altitude Ecosystem in the Qualities of Different Genetic Varieties Brassica napus Resources |
| title_full_unstemmed | Microscopical Observation and Transcriptome Analysis Reveal the Effects of High‐Altitude Ecosystem in the Qualities of Different Genetic Varieties Brassica napus Resources |
| title_short | Microscopical Observation and Transcriptome Analysis Reveal the Effects of High‐Altitude Ecosystem in the Qualities of Different Genetic Varieties Brassica napus Resources |
| title_sort | microscopical observation and transcriptome analysis reveal the effects of high altitude ecosystem in the qualities of different genetic varieties brassica napus resources |
| topic | altitude biomass bolting Brassica napus nutritional quality picking period |
| url | https://doi.org/10.1002/ece3.70616 |
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