Enhancing Pearl oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm) performance by evaluating the influence of potassium humate and wheat straw on yield and biochemical attributes

Abstract This study investigated the effects of various combinations of potassium humate and raw wheat straw (RWS) treatments on the growth, yield, and quality of Pleurotus ostreatus (Jacq.) P. Kumm across two growing seasons. A total of seven treatment combinations (H0_RWS, H0.25_RWS, H0.5_RWS, H0....

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Main Authors: Sayed Hussein Abdelgalil, Esraa Mohamed, Islam I. Teiba, Sobhi F. Lamlom, Ahmed M. Abdelghany, Mohamed E. Shalaby
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Plant Biology
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Online Access:https://doi.org/10.1186/s12870-025-06988-8
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author Sayed Hussein Abdelgalil
Esraa Mohamed
Islam I. Teiba
Sobhi F. Lamlom
Ahmed M. Abdelghany
Mohamed E. Shalaby
author_facet Sayed Hussein Abdelgalil
Esraa Mohamed
Islam I. Teiba
Sobhi F. Lamlom
Ahmed M. Abdelghany
Mohamed E. Shalaby
author_sort Sayed Hussein Abdelgalil
collection DOAJ
description Abstract This study investigated the effects of various combinations of potassium humate and raw wheat straw (RWS) treatments on the growth, yield, and quality of Pleurotus ostreatus (Jacq.) P. Kumm across two growing seasons. A total of seven treatment combinations (H0_RWS, H0.25_RWS, H0.5_RWS, H0.75_RWS, H1_RWS, H1.25_RWS, and H1.5_RWS) were applied to evaluate eleven traits, including soluble sugar content (SSC), fruiting body yield (FBY), biological efficiency (BE), and biochemical attributes. The analysis of variance revealed significant effects of both season and treatment on all traits, with notable interactions. The H0.5_RWS treatment demonstrated the highest improvements, increasing SSC by 15.3%, FBY by 18.6%, and vitamin C (VC) by 12.4% compared to the control. Utilizing principal component analysis (PCA) showed that the first two components explained 89.5% of the variance, with strong associations between SSC, VC, FBY, total phenolic content (TPC), BE, and peroxidase activity (POD) on PC1. The H0.5_RWS treatment was identified as the most effective for enhancing these parameters. Heatmap and radar plot analyses further confirmed the positive influence of moderate potassium humate doses, while higher doses resulted in reduced benefits. Correlation analysis showed strong positive relationships between yield and both total sugars (R² = 0.99) and BE (R² = 0.97). The results emphasize the substantial importance of balanced potassium humate application in enhancing the production and nutritional quality of P. ostreatus, providing valuable insights to optimize the cultivation practices of this species.
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issn 1471-2229
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spelling doaj-art-ce087df5b87541389df876986946a01d2025-08-20T04:01:56ZengBMCBMC Plant Biology1471-22292025-07-0125111310.1186/s12870-025-06988-8Enhancing Pearl oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm) performance by evaluating the influence of potassium humate and wheat straw on yield and biochemical attributesSayed Hussein Abdelgalil0Esraa Mohamed1Islam I. Teiba2Sobhi F. Lamlom3Ahmed M. Abdelghany4Mohamed E. Shalaby5Central Laboratory of Organic Agriculture (CLOA), Agricultural Research Center (ARC)Horticulture Research Institute, Vegetable Cross Pollination Dep, Agricultural Research Center (ARC)Botany Department, Faculty of Agriculture, Tanta UniversityPlant Production Department, Faculty of Agriculture Saba Basha, Alexandria UniversityCrop Science Department, Faculty of Agriculture, Damanhur UniversityPlant Production Department, Faculty of Agriculture Saba Basha, Alexandria UniversityAbstract This study investigated the effects of various combinations of potassium humate and raw wheat straw (RWS) treatments on the growth, yield, and quality of Pleurotus ostreatus (Jacq.) P. Kumm across two growing seasons. A total of seven treatment combinations (H0_RWS, H0.25_RWS, H0.5_RWS, H0.75_RWS, H1_RWS, H1.25_RWS, and H1.5_RWS) were applied to evaluate eleven traits, including soluble sugar content (SSC), fruiting body yield (FBY), biological efficiency (BE), and biochemical attributes. The analysis of variance revealed significant effects of both season and treatment on all traits, with notable interactions. The H0.5_RWS treatment demonstrated the highest improvements, increasing SSC by 15.3%, FBY by 18.6%, and vitamin C (VC) by 12.4% compared to the control. Utilizing principal component analysis (PCA) showed that the first two components explained 89.5% of the variance, with strong associations between SSC, VC, FBY, total phenolic content (TPC), BE, and peroxidase activity (POD) on PC1. The H0.5_RWS treatment was identified as the most effective for enhancing these parameters. Heatmap and radar plot analyses further confirmed the positive influence of moderate potassium humate doses, while higher doses resulted in reduced benefits. Correlation analysis showed strong positive relationships between yield and both total sugars (R² = 0.99) and BE (R² = 0.97). The results emphasize the substantial importance of balanced potassium humate application in enhancing the production and nutritional quality of P. ostreatus, providing valuable insights to optimize the cultivation practices of this species.https://doi.org/10.1186/s12870-025-06988-8Pleurotus ostreatusSubstrate amendmentGrowth optimizationPotassium humateBioactive compounds
spellingShingle Sayed Hussein Abdelgalil
Esraa Mohamed
Islam I. Teiba
Sobhi F. Lamlom
Ahmed M. Abdelghany
Mohamed E. Shalaby
Enhancing Pearl oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm) performance by evaluating the influence of potassium humate and wheat straw on yield and biochemical attributes
BMC Plant Biology
Pleurotus ostreatus
Substrate amendment
Growth optimization
Potassium humate
Bioactive compounds
title Enhancing Pearl oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm) performance by evaluating the influence of potassium humate and wheat straw on yield and biochemical attributes
title_full Enhancing Pearl oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm) performance by evaluating the influence of potassium humate and wheat straw on yield and biochemical attributes
title_fullStr Enhancing Pearl oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm) performance by evaluating the influence of potassium humate and wheat straw on yield and biochemical attributes
title_full_unstemmed Enhancing Pearl oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm) performance by evaluating the influence of potassium humate and wheat straw on yield and biochemical attributes
title_short Enhancing Pearl oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm) performance by evaluating the influence of potassium humate and wheat straw on yield and biochemical attributes
title_sort enhancing pearl oyster mushroom pleurotus ostreatus jacq p kumm performance by evaluating the influence of potassium humate and wheat straw on yield and biochemical attributes
topic Pleurotus ostreatus
Substrate amendment
Growth optimization
Potassium humate
Bioactive compounds
url https://doi.org/10.1186/s12870-025-06988-8
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