Multiomics reveal biomolecular shifts and ER stress in sleep-restricted women affecting NSC functions
Summary: Adequate sleep (AS) is vital for physiological functions, yet a third of US adults sleep less than recommended. While circadian rhythms regulate adult stem cell functions, the impact of insufficient sleep remains unclear. We previously completed a clinical trial in healthy women in a random...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-05-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225007710 |
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| author | Vikas Malik Xin Huang Hongwei Zhou Rebecca Bojar Rajesh Kumar Soni Donald W. Landry Sanja Jelic Jianlong Wang |
| author_facet | Vikas Malik Xin Huang Hongwei Zhou Rebecca Bojar Rajesh Kumar Soni Donald W. Landry Sanja Jelic Jianlong Wang |
| author_sort | Vikas Malik |
| collection | DOAJ |
| description | Summary: Adequate sleep (AS) is vital for physiological functions, yet a third of US adults sleep less than recommended. While circadian rhythms regulate adult stem cell functions, the impact of insufficient sleep remains unclear. We previously completed a clinical trial in healthy women in a randomized crossover design of 6-week periods with AS or mildly restricted sleep (RS; 1.5 h less). Here, we performed metabolomic and proteomic profiling of plasma samples. RS induced a stress-like state, highlighted by ER stress, heat shock, ubiquitination proteins, and amino acid biosynthesis. RS was strongly linked to disrupted neural development. Treating neural stem cells (NSCs) derived from human embryonic stem cells with RS-enriched metabolites disrupted G1 cell cycle phase and impaired differentiation into neurons, astrocytes, and oligodendrocytes. Our findings reveal how mild RS, mimicking “real-life” conditions, disrupts NSC divisions and differentiation, highlighting the critical role of sleep in adult stem cell regulation and neural development. |
| format | Article |
| id | doaj-art-ce0726b5078340fea9ba140c4a8d7de3 |
| institution | OA Journals |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-ce0726b5078340fea9ba140c4a8d7de32025-08-20T01:49:08ZengElsevieriScience2589-00422025-05-0128511251010.1016/j.isci.2025.112510Multiomics reveal biomolecular shifts and ER stress in sleep-restricted women affecting NSC functionsVikas Malik0Xin Huang1Hongwei Zhou2Rebecca Bojar3Rajesh Kumar Soni4Donald W. Landry5Sanja Jelic6Jianlong Wang7Department of Medicine, Columbia Center for Human Development and Stem Cell Therapies, Columbia Stem Cell Initiative, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USADepartment of Medicine, Columbia Center for Human Development and Stem Cell Therapies, Columbia Stem Cell Initiative, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USADepartment of Medicine, Columbia Center for Human Development and Stem Cell Therapies, Columbia Stem Cell Initiative, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USABarnard College, Columbia University, New York, NY 10027, USAProteomics and Macromolecular Crystallography Shared Resource, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USADivision of Nephrology, Department of Medicine, Columbia University Vagelos College of Physicians and Surgeons, New York-Presbyterian/Columbia University Irving Medical Center, New York, NY 10032, USADivision of Pulmonary, Allergy, and Critical Care Medicine, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032, USADepartment of Medicine, Columbia Center for Human Development and Stem Cell Therapies, Columbia Stem Cell Initiative, Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 10032, USA; Corresponding authorSummary: Adequate sleep (AS) is vital for physiological functions, yet a third of US adults sleep less than recommended. While circadian rhythms regulate adult stem cell functions, the impact of insufficient sleep remains unclear. We previously completed a clinical trial in healthy women in a randomized crossover design of 6-week periods with AS or mildly restricted sleep (RS; 1.5 h less). Here, we performed metabolomic and proteomic profiling of plasma samples. RS induced a stress-like state, highlighted by ER stress, heat shock, ubiquitination proteins, and amino acid biosynthesis. RS was strongly linked to disrupted neural development. Treating neural stem cells (NSCs) derived from human embryonic stem cells with RS-enriched metabolites disrupted G1 cell cycle phase and impaired differentiation into neurons, astrocytes, and oligodendrocytes. Our findings reveal how mild RS, mimicking “real-life” conditions, disrupts NSC divisions and differentiation, highlighting the critical role of sleep in adult stem cell regulation and neural development.http://www.sciencedirect.com/science/article/pii/S2589004225007710NeuroscienceStem cells researchOmics |
| spellingShingle | Vikas Malik Xin Huang Hongwei Zhou Rebecca Bojar Rajesh Kumar Soni Donald W. Landry Sanja Jelic Jianlong Wang Multiomics reveal biomolecular shifts and ER stress in sleep-restricted women affecting NSC functions iScience Neuroscience Stem cells research Omics |
| title | Multiomics reveal biomolecular shifts and ER stress in sleep-restricted women affecting NSC functions |
| title_full | Multiomics reveal biomolecular shifts and ER stress in sleep-restricted women affecting NSC functions |
| title_fullStr | Multiomics reveal biomolecular shifts and ER stress in sleep-restricted women affecting NSC functions |
| title_full_unstemmed | Multiomics reveal biomolecular shifts and ER stress in sleep-restricted women affecting NSC functions |
| title_short | Multiomics reveal biomolecular shifts and ER stress in sleep-restricted women affecting NSC functions |
| title_sort | multiomics reveal biomolecular shifts and er stress in sleep restricted women affecting nsc functions |
| topic | Neuroscience Stem cells research Omics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225007710 |
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