Astaxanthin mediated repair of tBHP-Induced cellular injury in chondrocytes
Objective This study investigates how astaxanthin (AST) counters tert-butyl hydroperoxide (tBHP)-induced cellular damage in C28/I2 chondrocytes, focusing on the circ-HP1BP3/miR-139-5p/SOD1 signaling pathway and its use in sustained-release microspheres for osteoarthritis treatment.Methods We employe...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Redox Report |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13510002.2024.2422271 |
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| _version_ | 1850167803354546176 |
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| author | Wenwei Liang Gang Liu Weibo Zhou Wei Chen Yaojun Lu Hao Wu Yao Qin Chunhui Zhu |
| author_facet | Wenwei Liang Gang Liu Weibo Zhou Wei Chen Yaojun Lu Hao Wu Yao Qin Chunhui Zhu |
| author_sort | Wenwei Liang |
| collection | DOAJ |
| description | Objective This study investigates how astaxanthin (AST) counters tert-butyl hydroperoxide (tBHP)-induced cellular damage in C28/I2 chondrocytes, focusing on the circ-HP1BP3/miR-139-5p/SOD1 signaling pathway and its use in sustained-release microspheres for osteoarthritis treatment.Methods We employed a variety of techniques including real-time quantitative PCR, Western blot, ELISA, and dual-luciferase reporter gene assays to explore AST's molecular effects. Additionally, the efficacy of AST-loaded sustained-release microspheres was evaluated in vitro and in a mouse model of osteoarthritis.Results AST significantly enhanced SOD1 expression, reducing apoptosis and inflammation in damaged cells. The AST-loaded microspheres showed promising in vitro drug release, improved cell viability, and reduced oxidative stress. In the osteoarthritis mouse model, they effectively decreased joint inflammation and increased the expression of chondrocyte markers.Conclusion Astaxanthin effectively mitigates oxidative stress and inflammation in chondrocytes via the circ-HP1BP3/miR-139-5p/SOD1 pathway. The development of AST-loaded microspheres offers a novel and promising approach for osteoarthritis therapy, potentially extending to osteoarthritis treatment. |
| format | Article |
| id | doaj-art-ce04bd9da9c4486384ea230cd30ee31b |
| institution | OA Journals |
| issn | 1351-0002 1743-2928 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Redox Report |
| spelling | doaj-art-ce04bd9da9c4486384ea230cd30ee31b2025-08-20T02:21:07ZengTaylor & Francis GroupRedox Report1351-00021743-29282024-12-0129110.1080/13510002.2024.2422271Astaxanthin mediated repair of tBHP-Induced cellular injury in chondrocytesWenwei Liang0Gang Liu1Weibo Zhou2Wei Chen3Yaojun Lu4Hao Wu5Yao Qin6Chunhui Zhu7Department of Orthopedics, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of ChinaDepartment of Orthopedics, The People's Hospital of Puyang, Puyang, People’s Republic of ChinaTrauma Center, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, Changzhou, People’s Republic of ChinaTrauma Center, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, Changzhou, People’s Republic of ChinaTrauma Center, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, Changzhou, People’s Republic of ChinaTrauma Center, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, Changzhou, People’s Republic of ChinaDepartment of Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, People’s Republic of ChinaTrauma Center, The Affiliated Changzhou Second People's Hospital of Nanjing Medical University, Changzhou, People’s Republic of ChinaObjective This study investigates how astaxanthin (AST) counters tert-butyl hydroperoxide (tBHP)-induced cellular damage in C28/I2 chondrocytes, focusing on the circ-HP1BP3/miR-139-5p/SOD1 signaling pathway and its use in sustained-release microspheres for osteoarthritis treatment.Methods We employed a variety of techniques including real-time quantitative PCR, Western blot, ELISA, and dual-luciferase reporter gene assays to explore AST's molecular effects. Additionally, the efficacy of AST-loaded sustained-release microspheres was evaluated in vitro and in a mouse model of osteoarthritis.Results AST significantly enhanced SOD1 expression, reducing apoptosis and inflammation in damaged cells. The AST-loaded microspheres showed promising in vitro drug release, improved cell viability, and reduced oxidative stress. In the osteoarthritis mouse model, they effectively decreased joint inflammation and increased the expression of chondrocyte markers.Conclusion Astaxanthin effectively mitigates oxidative stress and inflammation in chondrocytes via the circ-HP1BP3/miR-139-5p/SOD1 pathway. The development of AST-loaded microspheres offers a novel and promising approach for osteoarthritis therapy, potentially extending to osteoarthritis treatment.https://www.tandfonline.com/doi/10.1080/13510002.2024.2422271AstaxanthinC28/I2 cellstBHPCirc-HP1BP3Mir-139-5pSOD1 |
| spellingShingle | Wenwei Liang Gang Liu Weibo Zhou Wei Chen Yaojun Lu Hao Wu Yao Qin Chunhui Zhu Astaxanthin mediated repair of tBHP-Induced cellular injury in chondrocytes Redox Report Astaxanthin C28/I2 cells tBHP Circ-HP1BP3 Mir-139-5p SOD1 |
| title | Astaxanthin mediated repair of tBHP-Induced cellular injury in chondrocytes |
| title_full | Astaxanthin mediated repair of tBHP-Induced cellular injury in chondrocytes |
| title_fullStr | Astaxanthin mediated repair of tBHP-Induced cellular injury in chondrocytes |
| title_full_unstemmed | Astaxanthin mediated repair of tBHP-Induced cellular injury in chondrocytes |
| title_short | Astaxanthin mediated repair of tBHP-Induced cellular injury in chondrocytes |
| title_sort | astaxanthin mediated repair of tbhp induced cellular injury in chondrocytes |
| topic | Astaxanthin C28/I2 cells tBHP Circ-HP1BP3 Mir-139-5p SOD1 |
| url | https://www.tandfonline.com/doi/10.1080/13510002.2024.2422271 |
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