LC-MS-based metabolomics for detecting adulteration in Tribulus terrestris-derived dietary supplements
The widespread usage of Tribulus terrestris dietary supplements has elicited concerns over product authenticity and possible adulteration. This research utilized an untargeted liquid chromatography-high resolution mass spectrometry (LC-HRMS) metabolomics methodology to assess the composition of T. t...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-04-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525003232 |
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| author | Dejan Gođevac Jovana Stanković Jeremić Mirjana Cvetković Katarina Simić Ivana Sofrenić Jovana Ljujić Lazar Popović Uroš Gašić Yen-Nhi Hoang Tao Huan Stefan Ivanović |
| author_facet | Dejan Gođevac Jovana Stanković Jeremić Mirjana Cvetković Katarina Simić Ivana Sofrenić Jovana Ljujić Lazar Popović Uroš Gašić Yen-Nhi Hoang Tao Huan Stefan Ivanović |
| author_sort | Dejan Gođevac |
| collection | DOAJ |
| description | The widespread usage of Tribulus terrestris dietary supplements has elicited concerns over product authenticity and possible adulteration. This research utilized an untargeted liquid chromatography-high resolution mass spectrometry (LC-HRMS) metabolomics methodology to assess the composition of T. terrestris-derived supplements. Authentic plant materials, simulated adulterated samples, and commercial products were analyzed using principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA), and a convolutional neural network tool. The presence of PDE5 inhibitors and anabolic steroids in spiked samples was confirmed. Certain commercial products included undisclosed green tea and citrus-derived chemicals, likely incorporated to amplify stimulant effects and support testosterone-enhancing claims. Additionally, spirost-4-ene-3,12-dione was recognized as an indicator of possible steroidal saponin decomposition resulting from storage or processing conditions. This study illustrates the efficacy of LC-HRMS metabolomics in detecting supplement adulteration and emphasizes the need for rigorous quality control protocols to guarantee customer safety and product integrity. |
| format | Article |
| id | doaj-art-cdf2c9c949fa4db29b809c2982c14f5e |
| institution | OA Journals |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-cdf2c9c949fa4db29b809c2982c14f5e2025-08-20T02:31:51ZengElsevierFood Chemistry: X2590-15752025-04-012710247610.1016/j.fochx.2025.102476LC-MS-based metabolomics for detecting adulteration in Tribulus terrestris-derived dietary supplementsDejan Gođevac0Jovana Stanković Jeremić1Mirjana Cvetković2Katarina Simić3Ivana Sofrenić4Jovana Ljujić5Lazar Popović6Uroš Gašić7Yen-Nhi Hoang8Tao Huan9Stefan Ivanović10University of Belgrade, Institute of Chemistry, Technology and Metallurgy – National Institute of the Republic of Serbia, Njegoševa 12, 11000 Belgrade, Serbia; Corresponding author.University of Belgrade, Institute of Chemistry, Technology and Metallurgy – National Institute of the Republic of Serbia, Njegoševa 12, 11000 Belgrade, SerbiaUniversity of Belgrade, Institute of Chemistry, Technology and Metallurgy – National Institute of the Republic of Serbia, Njegoševa 12, 11000 Belgrade, SerbiaUniversity of Belgrade, Institute of Chemistry, Technology and Metallurgy – National Institute of the Republic of Serbia, Njegoševa 12, 11000 Belgrade, SerbiaUniversity of Belgrade – Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade, SerbiaUniversity of Belgrade – Faculty of Chemistry, Studentski trg 12-16, 11000 Belgrade, SerbiaUniversity of Belgrade – Innovation Center of Faculty of Chemistry, Studentski trg 16, 11000 Belgrade, SerbiaUniversity of Belgrade – Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, Bulevar Despota Stefana 142, 11000 Belgrade, SerbiaDepartment of Chemistry, Faculty of Science, University of British Columbia, Vancouver Campus, 2036 Main Mall, Vancouver, BC V6T 1Z1, CanadaDepartment of Chemistry, Faculty of Science, University of British Columbia, Vancouver Campus, 2036 Main Mall, Vancouver, BC V6T 1Z1, CanadaUniversity of Belgrade, Institute of Chemistry, Technology and Metallurgy – National Institute of the Republic of Serbia, Njegoševa 12, 11000 Belgrade, SerbiaThe widespread usage of Tribulus terrestris dietary supplements has elicited concerns over product authenticity and possible adulteration. This research utilized an untargeted liquid chromatography-high resolution mass spectrometry (LC-HRMS) metabolomics methodology to assess the composition of T. terrestris-derived supplements. Authentic plant materials, simulated adulterated samples, and commercial products were analyzed using principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA), and a convolutional neural network tool. The presence of PDE5 inhibitors and anabolic steroids in spiked samples was confirmed. Certain commercial products included undisclosed green tea and citrus-derived chemicals, likely incorporated to amplify stimulant effects and support testosterone-enhancing claims. Additionally, spirost-4-ene-3,12-dione was recognized as an indicator of possible steroidal saponin decomposition resulting from storage or processing conditions. This study illustrates the efficacy of LC-HRMS metabolomics in detecting supplement adulteration and emphasizes the need for rigorous quality control protocols to guarantee customer safety and product integrity.http://www.sciencedirect.com/science/article/pii/S2590157525003232Tribulus terrestrisMetabolomicsLC-HRMSDietary supplementsAdulteration |
| spellingShingle | Dejan Gođevac Jovana Stanković Jeremić Mirjana Cvetković Katarina Simić Ivana Sofrenić Jovana Ljujić Lazar Popović Uroš Gašić Yen-Nhi Hoang Tao Huan Stefan Ivanović LC-MS-based metabolomics for detecting adulteration in Tribulus terrestris-derived dietary supplements Food Chemistry: X Tribulus terrestris Metabolomics LC-HRMS Dietary supplements Adulteration |
| title | LC-MS-based metabolomics for detecting adulteration in Tribulus terrestris-derived dietary supplements |
| title_full | LC-MS-based metabolomics for detecting adulteration in Tribulus terrestris-derived dietary supplements |
| title_fullStr | LC-MS-based metabolomics for detecting adulteration in Tribulus terrestris-derived dietary supplements |
| title_full_unstemmed | LC-MS-based metabolomics for detecting adulteration in Tribulus terrestris-derived dietary supplements |
| title_short | LC-MS-based metabolomics for detecting adulteration in Tribulus terrestris-derived dietary supplements |
| title_sort | lc ms based metabolomics for detecting adulteration in tribulus terrestris derived dietary supplements |
| topic | Tribulus terrestris Metabolomics LC-HRMS Dietary supplements Adulteration |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525003232 |
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