The Association of Neuroendocrine Differentiation with MicroRNA 21 and MicroRNA let7f Expression and the Clinicopathological Parameters in Primary Invasive Breast Carcinomas with Neuroendocrine Features
MiRNAs have been reported as biomarkers with diagnostic, prognostic, and predictive value for many different diseases. Therapeutic agents targeting some miRNAs are currently being developed. We aimed to compare BC-NEFs (carcinoma of the breast with neuroendocrine features) with IDC (invasive ductal...
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2024-10-01
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| author | Gamze Usul Esra Canan Kelten Talu İsmail Yılmaz Gizem Narlı Issın Sibel Bektaş Didem Can Trabulus |
| author_facet | Gamze Usul Esra Canan Kelten Talu İsmail Yılmaz Gizem Narlı Issın Sibel Bektaş Didem Can Trabulus |
| author_sort | Gamze Usul |
| collection | DOAJ |
| description | MiRNAs have been reported as biomarkers with diagnostic, prognostic, and predictive value for many different diseases. Therapeutic agents targeting some miRNAs are currently being developed. We aimed to compare BC-NEFs (carcinoma of the breast with neuroendocrine features) with IDC (invasive ductal carcinoma) cases without neuroendocrine features in terms of the level of miRNA expression known to show the oncogenic (miR-21) and tumor-suppressor effects (miR-let7f) and the clinicopathological features. A total of 29 patients with a diagnosis of BC-NEFs (15 cases with neuroendocrine differentiation >50% of the whole section of tumor and 14 cases with neuroendocrine differentiation 10–50% of the tumor) and 30 patients with a diagnosis of IDC (no neuroendocrine differentiation) were retrospectively re-evaluated. Expression levels of miR-21 and miR-let7f were determined by the qRT-PCR method in paraffin tissue blocks. MiR-21 expression was significantly higher in the IDC group than in the group with BC-NEFs. miR-let7f expression was significantly lower in the group with BC-NEFs compared to the IDC group. A high expression level of miR-21 was found to be associated with progesterone receptor (PR) negativity. Our findings show that the presence of NEFs in breast carcinomas makes a significant difference in the expression levels of the investigated oncogenic (miR-21) and tumor-suppressor (miR-let7f) miRNAs. These findings suggest that miRNAs may be a potential biomarker in BC-NEFs and would benefit from targeted therapy. |
| format | Article |
| id | doaj-art-cde1e65bc00a4fc69f27427f5ce51999 |
| institution | OA Journals |
| issn | 2075-4418 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj-art-cde1e65bc00a4fc69f27427f5ce519992025-08-20T01:47:45ZengMDPI AGDiagnostics2075-44182024-10-011419221110.3390/diagnostics14192211The Association of Neuroendocrine Differentiation with MicroRNA 21 and MicroRNA let7f Expression and the Clinicopathological Parameters in Primary Invasive Breast Carcinomas with Neuroendocrine FeaturesGamze Usul0Esra Canan Kelten Talu1İsmail Yılmaz2Gizem Narlı Issın3Sibel Bektaş4Didem Can Trabulus5Department of Pathology, Istanbul Training and Research Hospital, Hamidiye Faculty of Medicine, University of Health Sciences, Istanbul 34098, TürkiyeDepartment of Pathology, Istanbul Training and Research Hospital, Hamidiye Faculty of Medicine, University of Health Sciences, Istanbul 34098, TürkiyeDepartment of Pathology, Sultan Abdülhamid Han Training and Research Hospital, Hamidiye Faculty of Medicine, University of Health Sciences, Istanbul 34668, TürkiyeMengücek Gazi Education and Research Hospital, Faculty of Medicine, Binali Yıldırım University, Erzincan 24180, TürkiyeDepartment of Pathology, Gaziosmanpaşa Training and Research Hospital, Hamidiye Faculty of Medicine, University of Health Sciences, Istanbul 34255, TürkiyeDepartment of General Surgery, Istanbul Training and Research Hospital, Hamidiye Faculty of Medicine, University of Health Sciences, Istanbul 34098, TürkiyeMiRNAs have been reported as biomarkers with diagnostic, prognostic, and predictive value for many different diseases. Therapeutic agents targeting some miRNAs are currently being developed. We aimed to compare BC-NEFs (carcinoma of the breast with neuroendocrine features) with IDC (invasive ductal carcinoma) cases without neuroendocrine features in terms of the level of miRNA expression known to show the oncogenic (miR-21) and tumor-suppressor effects (miR-let7f) and the clinicopathological features. A total of 29 patients with a diagnosis of BC-NEFs (15 cases with neuroendocrine differentiation >50% of the whole section of tumor and 14 cases with neuroendocrine differentiation 10–50% of the tumor) and 30 patients with a diagnosis of IDC (no neuroendocrine differentiation) were retrospectively re-evaluated. Expression levels of miR-21 and miR-let7f were determined by the qRT-PCR method in paraffin tissue blocks. MiR-21 expression was significantly higher in the IDC group than in the group with BC-NEFs. miR-let7f expression was significantly lower in the group with BC-NEFs compared to the IDC group. A high expression level of miR-21 was found to be associated with progesterone receptor (PR) negativity. Our findings show that the presence of NEFs in breast carcinomas makes a significant difference in the expression levels of the investigated oncogenic (miR-21) and tumor-suppressor (miR-let7f) miRNAs. These findings suggest that miRNAs may be a potential biomarker in BC-NEFs and would benefit from targeted therapy.https://www.mdpi.com/2075-4418/14/19/2211breast carcinoma with neuroendocrine featuresmiRNAmiR-21miR-let7fbiomarkerparaffin tissue blocks |
| spellingShingle | Gamze Usul Esra Canan Kelten Talu İsmail Yılmaz Gizem Narlı Issın Sibel Bektaş Didem Can Trabulus The Association of Neuroendocrine Differentiation with MicroRNA 21 and MicroRNA let7f Expression and the Clinicopathological Parameters in Primary Invasive Breast Carcinomas with Neuroendocrine Features Diagnostics breast carcinoma with neuroendocrine features miRNA miR-21 miR-let7f biomarker paraffin tissue blocks |
| title | The Association of Neuroendocrine Differentiation with MicroRNA 21 and MicroRNA let7f Expression and the Clinicopathological Parameters in Primary Invasive Breast Carcinomas with Neuroendocrine Features |
| title_full | The Association of Neuroendocrine Differentiation with MicroRNA 21 and MicroRNA let7f Expression and the Clinicopathological Parameters in Primary Invasive Breast Carcinomas with Neuroendocrine Features |
| title_fullStr | The Association of Neuroendocrine Differentiation with MicroRNA 21 and MicroRNA let7f Expression and the Clinicopathological Parameters in Primary Invasive Breast Carcinomas with Neuroendocrine Features |
| title_full_unstemmed | The Association of Neuroendocrine Differentiation with MicroRNA 21 and MicroRNA let7f Expression and the Clinicopathological Parameters in Primary Invasive Breast Carcinomas with Neuroendocrine Features |
| title_short | The Association of Neuroendocrine Differentiation with MicroRNA 21 and MicroRNA let7f Expression and the Clinicopathological Parameters in Primary Invasive Breast Carcinomas with Neuroendocrine Features |
| title_sort | association of neuroendocrine differentiation with microrna 21 and microrna let7f expression and the clinicopathological parameters in primary invasive breast carcinomas with neuroendocrine features |
| topic | breast carcinoma with neuroendocrine features miRNA miR-21 miR-let7f biomarker paraffin tissue blocks |
| url | https://www.mdpi.com/2075-4418/14/19/2211 |
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