The Optimization of In Vitro Culture Establishment and Shoot Proliferation of “GiSelA 17” (<i>Prunus canescens</i> × <i>Prunus avium</i>): A Novel Cherry Rootstock
“GiSelA 17” (<i>Prunus canescens</i> × <i>Prunus avium</i>) is a novel cherry clonal rootstock with the ability to bear fruit early and resist replant situations, and it has a high tolerance to the menaces of Prunus dwarf virus (PDV) and Prunus necrotic ring spot virus (PNRSV...
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2025-03-01
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| author | Ikra Manzoor Khalid Mushtaq Bhat Mohammad Amin Mir Narendran M. Nair Aashiq Hussain Pandit Ume Kulsum Shoeb Quadri Smithal Deshmukh Taras Pasternak |
| author_facet | Ikra Manzoor Khalid Mushtaq Bhat Mohammad Amin Mir Narendran M. Nair Aashiq Hussain Pandit Ume Kulsum Shoeb Quadri Smithal Deshmukh Taras Pasternak |
| author_sort | Ikra Manzoor |
| collection | DOAJ |
| description | “GiSelA 17” (<i>Prunus canescens</i> × <i>Prunus avium</i>) is a novel cherry clonal rootstock with the ability to bear fruit early and resist replant situations, and it has a high tolerance to the menaces of Prunus dwarf virus (PDV) and Prunus necrotic ring spot virus (PNRSV). In this study, two kinds of explants were taken, i.e., shoot tip (E1) (10 mm) and nodal segment (E2) (15 mm) explants. Five different sterilant regimes using sodium hypochlorite, mercuric chloride, and ethyl alcohol were employed to assess surface sterilization. Two types of media, namely Murashige and Skoog (MS) and Woody Plant Medium (WPM), and twelve and six plant growth regulator combinations with benzyl amino purine (BAP) and indole-3-butyric acid (IBA) were used, respectively, for the establishment and proliferation steps. The results show that maximum culture asepsis (75.33%) was obtained with shoot tips (E1) using 0.05% HgCl<sub>2</sub> for 5 min + 70% ethanol for 10 s (S4), and maximum explant survival (80.33%) was observed in 0.1% HgCl<sub>2</sub> for 5 min (S1) for shoot tips (E1). The maximum establishment rate (83.33%) was found in shoot tips (E1) in MS medium with BAP + IBA (1 + 0.01 mg/L) during the establishment step, with a maximum proliferation rate of 92.00% obtained in MS and BAP (0.75 mg/L). Inferior establishment results (26.66%) were obtained in nodal segments (E2) using WPM and BAP + IBA (1.50 + 0.01 mg/L), with a low proliferation rate (68.66%) in WPM and BAP + IBA (0.25 + 0.01 mg/L). Nonetheless, our research is the first in vitro study on “GiSelA 17” rootstock that focuses on generating the best quality planting material for commercial cherry production. |
| format | Article |
| id | doaj-art-cde19e0259474d5e85f8de686b14ab4f |
| institution | Kabale University |
| issn | 2037-0164 |
| language | English |
| publishDate | 2025-03-01 |
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| series | International Journal of Plant Biology |
| spelling | doaj-art-cde19e0259474d5e85f8de686b14ab4f2025-08-20T03:43:22ZengMDPI AGInternational Journal of Plant Biology2037-01642025-03-011613310.3390/ijpb16010033The Optimization of In Vitro Culture Establishment and Shoot Proliferation of “GiSelA 17” (<i>Prunus canescens</i> × <i>Prunus avium</i>): A Novel Cherry RootstockIkra Manzoor0Khalid Mushtaq Bhat1Mohammad Amin Mir2Narendran M. Nair3Aashiq Hussain Pandit4Ume Kulsum5Shoeb Quadri6Smithal Deshmukh7Taras Pasternak8Division of Fruit Science, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar 190025, IndiaDivision of Fruit Science, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar 190025, IndiaDivision of Fruit Science, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar 190025, IndiaCommercial Tissue Culture & Nursery, Seven Star Fruits Private Limited—A Unit of Maharashtra Hybrid Seeds Co. (MahycoGrow), Jalna 431203, IndiaDivision of Fruit Science, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar 190025, IndiaDivision of Agricultural Statistics, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Shalimar, Srinagar 190025, IndiaCommercial Tissue Culture & Nursery, Seven Star Fruits Private Limited—A Unit of Maharashtra Hybrid Seeds Co. (MahycoGrow), Jalna 431203, IndiaCommercial Tissue Culture & Nursery, Seven Star Fruits Private Limited—A Unit of Maharashtra Hybrid Seeds Co. (MahycoGrow), Jalna 431203, IndiaInstituto de Bioingeniería, Universidad Miguel Hernandez, 03202 Elche, Spain“GiSelA 17” (<i>Prunus canescens</i> × <i>Prunus avium</i>) is a novel cherry clonal rootstock with the ability to bear fruit early and resist replant situations, and it has a high tolerance to the menaces of Prunus dwarf virus (PDV) and Prunus necrotic ring spot virus (PNRSV). In this study, two kinds of explants were taken, i.e., shoot tip (E1) (10 mm) and nodal segment (E2) (15 mm) explants. Five different sterilant regimes using sodium hypochlorite, mercuric chloride, and ethyl alcohol were employed to assess surface sterilization. Two types of media, namely Murashige and Skoog (MS) and Woody Plant Medium (WPM), and twelve and six plant growth regulator combinations with benzyl amino purine (BAP) and indole-3-butyric acid (IBA) were used, respectively, for the establishment and proliferation steps. The results show that maximum culture asepsis (75.33%) was obtained with shoot tips (E1) using 0.05% HgCl<sub>2</sub> for 5 min + 70% ethanol for 10 s (S4), and maximum explant survival (80.33%) was observed in 0.1% HgCl<sub>2</sub> for 5 min (S1) for shoot tips (E1). The maximum establishment rate (83.33%) was found in shoot tips (E1) in MS medium with BAP + IBA (1 + 0.01 mg/L) during the establishment step, with a maximum proliferation rate of 92.00% obtained in MS and BAP (0.75 mg/L). Inferior establishment results (26.66%) were obtained in nodal segments (E2) using WPM and BAP + IBA (1.50 + 0.01 mg/L), with a low proliferation rate (68.66%) in WPM and BAP + IBA (0.25 + 0.01 mg/L). Nonetheless, our research is the first in vitro study on “GiSelA 17” rootstock that focuses on generating the best quality planting material for commercial cherry production.https://www.mdpi.com/2037-0164/16/1/33cherryGiSelAproliferationringspotrootstockshoot tip |
| spellingShingle | Ikra Manzoor Khalid Mushtaq Bhat Mohammad Amin Mir Narendran M. Nair Aashiq Hussain Pandit Ume Kulsum Shoeb Quadri Smithal Deshmukh Taras Pasternak The Optimization of In Vitro Culture Establishment and Shoot Proliferation of “GiSelA 17” (<i>Prunus canescens</i> × <i>Prunus avium</i>): A Novel Cherry Rootstock International Journal of Plant Biology cherry GiSelA proliferation ringspot rootstock shoot tip |
| title | The Optimization of In Vitro Culture Establishment and Shoot Proliferation of “GiSelA 17” (<i>Prunus canescens</i> × <i>Prunus avium</i>): A Novel Cherry Rootstock |
| title_full | The Optimization of In Vitro Culture Establishment and Shoot Proliferation of “GiSelA 17” (<i>Prunus canescens</i> × <i>Prunus avium</i>): A Novel Cherry Rootstock |
| title_fullStr | The Optimization of In Vitro Culture Establishment and Shoot Proliferation of “GiSelA 17” (<i>Prunus canescens</i> × <i>Prunus avium</i>): A Novel Cherry Rootstock |
| title_full_unstemmed | The Optimization of In Vitro Culture Establishment and Shoot Proliferation of “GiSelA 17” (<i>Prunus canescens</i> × <i>Prunus avium</i>): A Novel Cherry Rootstock |
| title_short | The Optimization of In Vitro Culture Establishment and Shoot Proliferation of “GiSelA 17” (<i>Prunus canescens</i> × <i>Prunus avium</i>): A Novel Cherry Rootstock |
| title_sort | optimization of in vitro culture establishment and shoot proliferation of gisela 17 i prunus canescens i i prunus avium i a novel cherry rootstock |
| topic | cherry GiSelA proliferation ringspot rootstock shoot tip |
| url | https://www.mdpi.com/2037-0164/16/1/33 |
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