Supernatant of plant-associated bacteria potency against biofilms formed by foodborne pathogen and food spoilage bacteria
Abstract Objectives Food products are often contaminated by pathogens and spoilage bacteria. Most of them can form biofilms, a community of cells embedded in protective extracellular matrix layers resistant to harsh conditions, including antibiotics. Therefore, alternative antibiofilm agents are req...
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BMC
2024-11-01
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| Online Access: | https://doi.org/10.1186/s13104-024-06997-0 |
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| author | Gabriella Gandasurya Diana Elizabeth Waturangi Nurmaya Papuangan Nurhasanah Pande Gde Sasmita Julyantoro |
| author_facet | Gabriella Gandasurya Diana Elizabeth Waturangi Nurmaya Papuangan Nurhasanah Pande Gde Sasmita Julyantoro |
| author_sort | Gabriella Gandasurya |
| collection | DOAJ |
| description | Abstract Objectives Food products are often contaminated by pathogens and spoilage bacteria. Most of them can form biofilms, a community of cells embedded in protective extracellular matrix layers resistant to harsh conditions, including antibiotics. Therefore, alternative antibiofilm agents are required to overcome biofilm formation. This study aims to determine and quantify the antibiofilm activity of supernatants from plant-associated bacteria against biofilms of foodborne pathogen and food spoilage bacterium, namely Bacillus cereus and Bacillus subtilis. Results Plant-associated bacteria (PAB) have shown promising antibiofilm activities against biofilm-forming pathogens in previous studies. Thirteen PAB isolated from Ternate, Indonesia were used in this study. Supernatants of PAB were subjected to antimicrobial activity and quorum quenching detection, both using the well diffusion method. Four supernatants inhibited the growth of B. subtilis, but none affected the growth of B. cereus. Eight supernatants were able to disrupt the quorum sensing system of an indicator bacterium, wild-type Chromobacterium violaceum. Biofilm inhibition and destruction were quantified using 96-well microplates. The highest biofilm inhibition and destruction activities of PAB supernatants against each of B. cereus and B. subtilis biofilms were > 76%, and were later confirmed by light microscope and scanning electron microscope. Brine shrimp lethality assay (BSLA) was conducted and revealed that the selected PAB supernatants were non-toxic. The 16S rRNA gene of PAB were sequenced and they showed similarities to Bacillus, Priestia, and Chryseobacterium. Compounds in the supernatants were determined by GC–MS which revealed contents of fatty acids, ethyl esters, and diketopiperazines. Therefore, PAB supernatants have potential as antibiofilm agents against biofilm formed by Bacillus cereus and Bacillus subtilis. |
| format | Article |
| id | doaj-art-cdcfe328a7b74e7abe5712115e246790 |
| institution | Kabale University |
| issn | 1756-0500 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj-art-cdcfe328a7b74e7abe5712115e2467902024-11-17T12:08:19ZengBMCBMC Research Notes1756-05002024-11-011711810.1186/s13104-024-06997-0Supernatant of plant-associated bacteria potency against biofilms formed by foodborne pathogen and food spoilage bacteriaGabriella Gandasurya0Diana Elizabeth Waturangi1Nurmaya Papuangan2Nurhasanah3Pande Gde Sasmita Julyantoro4Department of Food Technology, Faculty of Biotechnology, Atma Jaya Catholic University of IndonesiaDepartment of Master in Biotechnology, Faculty of Biotechnology, Atma Jaya Catholic University of IndonesiaDepartment of Biology Education, Faculty of Teacher Training and Education, Khairun UniversityDepartment of Biology Education, Faculty of Teacher Training and Education, Khairun UniversityDepartment of Aquatic Resources Management, Faculty of Marine Science and Fisheries, University of UdayanaAbstract Objectives Food products are often contaminated by pathogens and spoilage bacteria. Most of them can form biofilms, a community of cells embedded in protective extracellular matrix layers resistant to harsh conditions, including antibiotics. Therefore, alternative antibiofilm agents are required to overcome biofilm formation. This study aims to determine and quantify the antibiofilm activity of supernatants from plant-associated bacteria against biofilms of foodborne pathogen and food spoilage bacterium, namely Bacillus cereus and Bacillus subtilis. Results Plant-associated bacteria (PAB) have shown promising antibiofilm activities against biofilm-forming pathogens in previous studies. Thirteen PAB isolated from Ternate, Indonesia were used in this study. Supernatants of PAB were subjected to antimicrobial activity and quorum quenching detection, both using the well diffusion method. Four supernatants inhibited the growth of B. subtilis, but none affected the growth of B. cereus. Eight supernatants were able to disrupt the quorum sensing system of an indicator bacterium, wild-type Chromobacterium violaceum. Biofilm inhibition and destruction were quantified using 96-well microplates. The highest biofilm inhibition and destruction activities of PAB supernatants against each of B. cereus and B. subtilis biofilms were > 76%, and were later confirmed by light microscope and scanning electron microscope. Brine shrimp lethality assay (BSLA) was conducted and revealed that the selected PAB supernatants were non-toxic. The 16S rRNA gene of PAB were sequenced and they showed similarities to Bacillus, Priestia, and Chryseobacterium. Compounds in the supernatants were determined by GC–MS which revealed contents of fatty acids, ethyl esters, and diketopiperazines. Therefore, PAB supernatants have potential as antibiofilm agents against biofilm formed by Bacillus cereus and Bacillus subtilis.https://doi.org/10.1186/s13104-024-06997-0BiofilmAntibiofilmPlant-associated bacteriaFoodborne pathogenFood spoilage bacteria |
| spellingShingle | Gabriella Gandasurya Diana Elizabeth Waturangi Nurmaya Papuangan Nurhasanah Pande Gde Sasmita Julyantoro Supernatant of plant-associated bacteria potency against biofilms formed by foodborne pathogen and food spoilage bacteria BMC Research Notes Biofilm Antibiofilm Plant-associated bacteria Foodborne pathogen Food spoilage bacteria |
| title | Supernatant of plant-associated bacteria potency against biofilms formed by foodborne pathogen and food spoilage bacteria |
| title_full | Supernatant of plant-associated bacteria potency against biofilms formed by foodborne pathogen and food spoilage bacteria |
| title_fullStr | Supernatant of plant-associated bacteria potency against biofilms formed by foodborne pathogen and food spoilage bacteria |
| title_full_unstemmed | Supernatant of plant-associated bacteria potency against biofilms formed by foodborne pathogen and food spoilage bacteria |
| title_short | Supernatant of plant-associated bacteria potency against biofilms formed by foodborne pathogen and food spoilage bacteria |
| title_sort | supernatant of plant associated bacteria potency against biofilms formed by foodborne pathogen and food spoilage bacteria |
| topic | Biofilm Antibiofilm Plant-associated bacteria Foodborne pathogen Food spoilage bacteria |
| url | https://doi.org/10.1186/s13104-024-06997-0 |
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