Genetic and Phenotypic Characterization of <i>Botrytis</i> Populations from Economic and Wild Host Plants in Iran

Grey mould disease, caused by various <i>Botrytis</i> species, poses a significant threat to important plants worldwide. This study aimed to characterize <i>Botrytis</i> populations on strawberry and roses, economically relevant host plants, and raspberry, used as a represent...

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Main Authors: Sepideh Fekrikohan, Bahram Sharifnabi, Mohammad Javan-Nikkhah, Stefania Pollastro, Francesco Faretra, Rita Milvia De Miccolis Angelini
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Journal of Fungi
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Online Access:https://www.mdpi.com/2309-608X/10/11/764
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author Sepideh Fekrikohan
Bahram Sharifnabi
Mohammad Javan-Nikkhah
Stefania Pollastro
Francesco Faretra
Rita Milvia De Miccolis Angelini
author_facet Sepideh Fekrikohan
Bahram Sharifnabi
Mohammad Javan-Nikkhah
Stefania Pollastro
Francesco Faretra
Rita Milvia De Miccolis Angelini
author_sort Sepideh Fekrikohan
collection DOAJ
description Grey mould disease, caused by various <i>Botrytis</i> species, poses a significant threat to important plants worldwide. This study aimed to characterize <i>Botrytis</i> populations on strawberry and roses, economically relevant host plants, and raspberry, used as a representative of wild plants, in Iran. A total of 389 isolates were collected and analyzed based on morphological features and haplotyping using molecular markers, transposable elements (<i>Boty</i> and <i>Flipper</i>), and fungicide response. Moreover, 60 isolates were used for phylogenetic analysis based on the <i>rpb2</i> gene, and 16 selected isolates from each clade were further characterized using the <i>g3pdh</i>, <i>hsp60</i>, and <i>nep2</i> genes. The results revealed the presence of three distinct species, <i>Botrytis cinerea</i>, <i>Botrytis sinoviticola</i>, and <i>Botrytis prunorum</i>, among the sampled isolates. Additionally, this study reports for the first time the presence of <i>B. sinoviticola</i> on strawberry and isolates belonging to <i>B. cinerea</i> group S in Iran. These findings provide insights into the diversity and composition of <i>Botrytis</i> populations on Iranian host plants.
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spelling doaj-art-cdcbc7613fc1421ca5c1f27fc0fbde4d2025-08-20T02:47:58ZengMDPI AGJournal of Fungi2309-608X2024-11-01101176410.3390/jof10110764Genetic and Phenotypic Characterization of <i>Botrytis</i> Populations from Economic and Wild Host Plants in IranSepideh Fekrikohan0Bahram Sharifnabi1Mohammad Javan-Nikkhah2Stefania Pollastro3Francesco Faretra4Rita Milvia De Miccolis Angelini5Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, IranDepartment of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, IranDepartment of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Tehran 1417466191, IranDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Bari 70126, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Bari 70126, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Bari 70126, ItalyGrey mould disease, caused by various <i>Botrytis</i> species, poses a significant threat to important plants worldwide. This study aimed to characterize <i>Botrytis</i> populations on strawberry and roses, economically relevant host plants, and raspberry, used as a representative of wild plants, in Iran. A total of 389 isolates were collected and analyzed based on morphological features and haplotyping using molecular markers, transposable elements (<i>Boty</i> and <i>Flipper</i>), and fungicide response. Moreover, 60 isolates were used for phylogenetic analysis based on the <i>rpb2</i> gene, and 16 selected isolates from each clade were further characterized using the <i>g3pdh</i>, <i>hsp60</i>, and <i>nep2</i> genes. The results revealed the presence of three distinct species, <i>Botrytis cinerea</i>, <i>Botrytis sinoviticola</i>, and <i>Botrytis prunorum</i>, among the sampled isolates. Additionally, this study reports for the first time the presence of <i>B. sinoviticola</i> on strawberry and isolates belonging to <i>B. cinerea</i> group S in Iran. These findings provide insights into the diversity and composition of <i>Botrytis</i> populations on Iranian host plants.https://www.mdpi.com/2309-608X/10/11/764grey mouldmolecular characterizationrosestrawberryraspberry
spellingShingle Sepideh Fekrikohan
Bahram Sharifnabi
Mohammad Javan-Nikkhah
Stefania Pollastro
Francesco Faretra
Rita Milvia De Miccolis Angelini
Genetic and Phenotypic Characterization of <i>Botrytis</i> Populations from Economic and Wild Host Plants in Iran
Journal of Fungi
grey mould
molecular characterization
rose
strawberry
raspberry
title Genetic and Phenotypic Characterization of <i>Botrytis</i> Populations from Economic and Wild Host Plants in Iran
title_full Genetic and Phenotypic Characterization of <i>Botrytis</i> Populations from Economic and Wild Host Plants in Iran
title_fullStr Genetic and Phenotypic Characterization of <i>Botrytis</i> Populations from Economic and Wild Host Plants in Iran
title_full_unstemmed Genetic and Phenotypic Characterization of <i>Botrytis</i> Populations from Economic and Wild Host Plants in Iran
title_short Genetic and Phenotypic Characterization of <i>Botrytis</i> Populations from Economic and Wild Host Plants in Iran
title_sort genetic and phenotypic characterization of i botrytis i populations from economic and wild host plants in iran
topic grey mould
molecular characterization
rose
strawberry
raspberry
url https://www.mdpi.com/2309-608X/10/11/764
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AT mohammadjavannikkhah geneticandphenotypiccharacterizationofibotrytisipopulationsfromeconomicandwildhostplantsiniran
AT stefaniapollastro geneticandphenotypiccharacterizationofibotrytisipopulationsfromeconomicandwildhostplantsiniran
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