In Vitro and In Silico Evaluation of the Anti-Aging Potential of <i>Eugenia uniflora</i> UAE Extracts
Skin aging is a natural biological process that can be accelerated by free radical induction, leading to a reduction in skin elasticity and the formation of wrinkles due to the depletion of elastin. <i>Eugenia uniflora</i> (dewandaru) is a promising plant believed to possess anti-aging p...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
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| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/30/15/3168 |
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| Summary: | Skin aging is a natural biological process that can be accelerated by free radical induction, leading to a reduction in skin elasticity and the formation of wrinkles due to the depletion of elastin. <i>Eugenia uniflora</i> (dewandaru) is a promising plant believed to possess anti-aging properties, primarily attributed to its major constituents, myricitrin and quercetin. This study aimed to investigate the anti-elastase and antioxidant properties of <i>Eugenia uniflora</i> stem bark, ripe fruit, and seed extracts. Extracts were obtained using an ultrasound-assisted extraction (UAE) method with 70% ethanol. Quantitative phytochemical analysis involved measuring the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity. Bioactive constituents were identified using LC-MS analysis, and their interactions with target enzymes were further evaluated through in silico molecular docking. The results demonstrated that the <i>E. uniflora</i> seed extract exhibited the highest antioxidant activity, with an IC<sub>50</sub> of 5.23 µg/mL (DPPH assay) and a FRAP value of 3233.32 µmol FeSO<sub>4</sub>/g. Furthermore, the ethanolic seed extract showed significant anti-elastase activity with an IC<sub>50</sub> of 114.14 µg/mL. Molecular docking predicted strong potential for several compounds as pancreatic elastase inhibitors, including 5-phenylvaleric acid, 2-(3-phenylpropyl)phenol, n-amylbenzene, 2-aminoadipic acid, and traumatin, each showing a prediction activity (PA) value exceeding 0.6. Notably, these compounds also exhibited inhibitory activity against tyrosinase. These findings collectively underscore the significant promise of <i>E. uniflora</i> seed extract as a novel and natural candidate for pharmacocosmeceutical product development, particularly for anti-aging applications. |
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| ISSN: | 1420-3049 |