Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test
Insulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of thi...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2015/102054 |
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| author | Feven Berhane Alemu Fite Nour Daboul Wissam Al-Janabi Zaher Msallaty Michael Caruso Monique K. Lewis Zhengping Yi Michael P. Diamond Abdul-Badi Abou-Samra Berhane Seyoum |
| author_facet | Feven Berhane Alemu Fite Nour Daboul Wissam Al-Janabi Zaher Msallaty Michael Caruso Monique K. Lewis Zhengping Yi Michael P. Diamond Abdul-Badi Abou-Samra Berhane Seyoum |
| author_sort | Feven Berhane |
| collection | DOAJ |
| description | Insulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of this study was to determine the pattern of circulating lactate levels during oral glucose tolerance test (OGTT) and hyperinsulinemic euglycemic clamp (HIEC) study in normal nondiabetic subjects. Lactate and glycerol were determined every 30 minutes during OGTT and HIEC on 22 participants. Lactate progressively increased throughout the HIEC study period (P<0.001). Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P<0.05). This trend also continued throughout the OGTT. In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P<0.001). In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D. |
| format | Article |
| id | doaj-art-cdbbf2c334a0423b8c613c2a2415aa1a |
| institution | DOAJ |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-cdbbf2c334a0423b8c613c2a2415aa1a2025-08-20T03:19:38ZengWileyJournal of Diabetes Research2314-67452314-67532015-01-01201510.1155/2015/102054102054Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance TestFeven Berhane0Alemu Fite1Nour Daboul2Wissam Al-Janabi3Zaher Msallaty4Michael Caruso5Monique K. Lewis6Zhengping Yi7Michael P. Diamond8Abdul-Badi Abou-Samra9Berhane Seyoum10Wayne State University School of Medicine, Detroit, MI 48201, USAWayne State University School of Medicine, Detroit, MI 48201, USAWayne State University School of Medicine, Detroit, MI 48201, USAWayne State University School of Medicine, Detroit, MI 48201, USAWayne State University School of Medicine, Detroit, MI 48201, USAWayne State University School of Medicine, Detroit, MI 48201, USAWayne State University School of Medicine, Detroit, MI 48201, USAWayne State University School of Medicine, Detroit, MI 48201, USAGeorgia Regents University, Augusta, GA 30912, USAHamad Medical Corporation, Doha, QatarWayne State University School of Medicine, Detroit, MI 48201, USAInsulin resistance, which plays a central role in the pathogenesis of type 2 diabetes (T2D), is an early indicator that heralds the occurrence of T2D. It is imperative to understand the metabolic changes that occur at the cellular level in the early stages of insulin resistance. The objective of this study was to determine the pattern of circulating lactate levels during oral glucose tolerance test (OGTT) and hyperinsulinemic euglycemic clamp (HIEC) study in normal nondiabetic subjects. Lactate and glycerol were determined every 30 minutes during OGTT and HIEC on 22 participants. Lactate progressively increased throughout the HIEC study period (P<0.001). Participants with BMI < 30 had significantly higher mean M-values compared to those with BMI ≥ 30 at baseline (P<0.05). This trend also continued throughout the OGTT. In addition, those with impaired glucose tolerance test (IGT) had significantly higher mean lactate levels compared to those with normal glucose tolerance (P<0.001). In conclusion, we found that lactate increased during HIEC study, which is a state of hyperinsulinemia similar to the metabolic milieu seen during the early stages in the development of T2D.http://dx.doi.org/10.1155/2015/102054 |
| spellingShingle | Feven Berhane Alemu Fite Nour Daboul Wissam Al-Janabi Zaher Msallaty Michael Caruso Monique K. Lewis Zhengping Yi Michael P. Diamond Abdul-Badi Abou-Samra Berhane Seyoum Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test Journal of Diabetes Research |
| title | Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test |
| title_full | Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test |
| title_fullStr | Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test |
| title_full_unstemmed | Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test |
| title_short | Plasma Lactate Levels Increase during Hyperinsulinemic Euglycemic Clamp and Oral Glucose Tolerance Test |
| title_sort | plasma lactate levels increase during hyperinsulinemic euglycemic clamp and oral glucose tolerance test |
| url | http://dx.doi.org/10.1155/2015/102054 |
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