Cloning, expression of carboxylesterase BioH and improvement of its hydrolysis activity by directed evolution
Carboxylesterase BioH of Escherichia coli is an important enzyme in the biotin synthesis. It contains a typical catalytic triad of Ser-His-Asp and displays significant carboxylesterase activities. The enzyme shows a broad substrate specificity with a preference for short acyl chain substrates. This...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2013-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2013.01.091 |
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