Molecular Characterization of miRNAs in <i>Myzus persicae</i> Carrying Brassica Yellows Virus
microRNAs (miRNAs) influence many biological processes at the post-transcriptional level. However, the molecular characterization of miRNAs in the <i>Myzus persicae</i> response to Brassica yellows virus (BrYV) stress remains unclear. In this study, we present the results of miRNA profil...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/13/11/941 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850145393480826880 |
|---|---|
| author | Meng-Jun He Yun Wang Mei Zhao Deng-Pan Zuo You Wang Zong-Ying Zhang Ying Wang Cheng-Gui Han |
| author_facet | Meng-Jun He Yun Wang Mei Zhao Deng-Pan Zuo You Wang Zong-Ying Zhang Ying Wang Cheng-Gui Han |
| author_sort | Meng-Jun He |
| collection | DOAJ |
| description | microRNAs (miRNAs) influence many biological processes at the post-transcriptional level. However, the molecular characterization of miRNAs in the <i>Myzus persicae</i> response to Brassica yellows virus (BrYV) stress remains unclear. In this study, we present the results of miRNA profiling in <i>Myzus persicae</i> under two different treatments: treatment one (raised on turnip plants), and treatment two (raised on <i>Arabidopsis thaliana</i>). A total of 72 known and 113 novel mature miRNAs were identified in both non-viruliferous and viruliferous aphids, under treatment one. In treatment two, 72 known and 112 novel mature miRNAs were identified in BrYV-free aphids; meanwhile, 71 known and 115 novel miRNAs were identified in BrYV-carrying aphids. Moreover, eight upregulated and four downregulated miRNAs were identified in viruliferous aphids under treatment two, whereas only two miRNAs were differentially expressed under treatment one. These results indicated the relative BrYV level could influence miRNA expression in aphids. KEGG enrichment analysis showed the predicted genes targeted by differentially expressed miRNAs were primarily involved in Peroxisome, neuroactive ligand–receptor interaction, and metabolism of xenobiotics by cytochrome P450 pathways. Taken together, these findings reveal the effect of BrYV on miRNAs in <i>Myzus persicae</i> and provide key clues for further studies on the molecular mechanisms of BrYV transmission via aphids. |
| format | Article |
| id | doaj-art-cdb6a305e2864b41ad8cb7fecf7143dc |
| institution | OA Journals |
| issn | 2079-7737 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj-art-cdb6a305e2864b41ad8cb7fecf7143dc2025-08-20T02:28:07ZengMDPI AGBiology2079-77372024-11-01131194110.3390/biology13110941Molecular Characterization of miRNAs in <i>Myzus persicae</i> Carrying Brassica Yellows VirusMeng-Jun He0Yun Wang1Mei Zhao2Deng-Pan Zuo3You Wang4Zong-Ying Zhang5Ying Wang6Cheng-Gui Han7Ministry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaMinistry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaMinistry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaMinistry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaMinistry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaMinistry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaMinistry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, ChinaMinistry of Agriculture and Rural Affairs Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, ChinamicroRNAs (miRNAs) influence many biological processes at the post-transcriptional level. However, the molecular characterization of miRNAs in the <i>Myzus persicae</i> response to Brassica yellows virus (BrYV) stress remains unclear. In this study, we present the results of miRNA profiling in <i>Myzus persicae</i> under two different treatments: treatment one (raised on turnip plants), and treatment two (raised on <i>Arabidopsis thaliana</i>). A total of 72 known and 113 novel mature miRNAs were identified in both non-viruliferous and viruliferous aphids, under treatment one. In treatment two, 72 known and 112 novel mature miRNAs were identified in BrYV-free aphids; meanwhile, 71 known and 115 novel miRNAs were identified in BrYV-carrying aphids. Moreover, eight upregulated and four downregulated miRNAs were identified in viruliferous aphids under treatment two, whereas only two miRNAs were differentially expressed under treatment one. These results indicated the relative BrYV level could influence miRNA expression in aphids. KEGG enrichment analysis showed the predicted genes targeted by differentially expressed miRNAs were primarily involved in Peroxisome, neuroactive ligand–receptor interaction, and metabolism of xenobiotics by cytochrome P450 pathways. Taken together, these findings reveal the effect of BrYV on miRNAs in <i>Myzus persicae</i> and provide key clues for further studies on the molecular mechanisms of BrYV transmission via aphids.https://www.mdpi.com/2079-7737/13/11/941<i>polerovirus</i>brassica yellows virusgreen peach aphidmicroRNAssRNA sequencingbioinformatics |
| spellingShingle | Meng-Jun He Yun Wang Mei Zhao Deng-Pan Zuo You Wang Zong-Ying Zhang Ying Wang Cheng-Gui Han Molecular Characterization of miRNAs in <i>Myzus persicae</i> Carrying Brassica Yellows Virus Biology <i>polerovirus</i> brassica yellows virus green peach aphid microRNAs sRNA sequencing bioinformatics |
| title | Molecular Characterization of miRNAs in <i>Myzus persicae</i> Carrying Brassica Yellows Virus |
| title_full | Molecular Characterization of miRNAs in <i>Myzus persicae</i> Carrying Brassica Yellows Virus |
| title_fullStr | Molecular Characterization of miRNAs in <i>Myzus persicae</i> Carrying Brassica Yellows Virus |
| title_full_unstemmed | Molecular Characterization of miRNAs in <i>Myzus persicae</i> Carrying Brassica Yellows Virus |
| title_short | Molecular Characterization of miRNAs in <i>Myzus persicae</i> Carrying Brassica Yellows Virus |
| title_sort | molecular characterization of mirnas in i myzus persicae i carrying brassica yellows virus |
| topic | <i>polerovirus</i> brassica yellows virus green peach aphid microRNAs sRNA sequencing bioinformatics |
| url | https://www.mdpi.com/2079-7737/13/11/941 |
| work_keys_str_mv | AT mengjunhe molecularcharacterizationofmirnasinimyzuspersicaeicarryingbrassicayellowsvirus AT yunwang molecularcharacterizationofmirnasinimyzuspersicaeicarryingbrassicayellowsvirus AT meizhao molecularcharacterizationofmirnasinimyzuspersicaeicarryingbrassicayellowsvirus AT dengpanzuo molecularcharacterizationofmirnasinimyzuspersicaeicarryingbrassicayellowsvirus AT youwang molecularcharacterizationofmirnasinimyzuspersicaeicarryingbrassicayellowsvirus AT zongyingzhang molecularcharacterizationofmirnasinimyzuspersicaeicarryingbrassicayellowsvirus AT yingwang molecularcharacterizationofmirnasinimyzuspersicaeicarryingbrassicayellowsvirus AT chengguihan molecularcharacterizationofmirnasinimyzuspersicaeicarryingbrassicayellowsvirus |