Plastic Responses of <i>Iris pumila</i> Functional and Mechanistic Leaf Traits to Experimental Warming

Phenotypic plasticity is an important adaptive strategy that enables plants to respond to environmental changes, particularly temperature fluctuations associated with global warming. In this study, the phenotypic plasticity of <i data-eusoft-scrollable-element="1">Iris pumila</i&g...

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Main Authors: Katarina Hočevar, Ana Vuleta, Sanja Manitašević Jovanović
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/6/960
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author Katarina Hočevar
Ana Vuleta
Sanja Manitašević Jovanović
author_facet Katarina Hočevar
Ana Vuleta
Sanja Manitašević Jovanović
author_sort Katarina Hočevar
collection DOAJ
description Phenotypic plasticity is an important adaptive strategy that enables plants to respond to environmental changes, particularly temperature fluctuations associated with global warming. In this study, the phenotypic plasticity of <i data-eusoft-scrollable-element="1">Iris pumila</i> leaf traits in response to an elevated temperature (by 1 °C) was investigated under controlled experimental conditions. In particular, we investigated important functional and mechanistic leaf traits: specific leaf area (SLA), leaf dry matter content (LDMC), specific leaf water content (SLWC), stomatal density (SD), leaf thickness (LT), and chlorophyll content. The results revealed that an elevated temperature induced trait-specific plastic responses, with mechanistic traits exhibiting greater plasticity than functional traits, reflecting their role in short-term acclimation. SLA and SD increased at higher temperatures, promoting photosynthesis and gas exchange, while reductions in SLWC, LDMC, LT, and chlorophyll content suggest a trade-off in favor of growth and metabolic activity over structural investment. Notably, chlorophyll content exhibited the highest plasticity, emphasizing its crucial role in modulating photosynthetic efficiency under thermal stress. Correlation analyses revealed strong phenotypic integration between leaf traits, with distinct trait relationships emerging under different temperature conditions. These findings suggest that <i data-eusoft-scrollable-element="1">I. pumila</i> employs both rapid physiological adjustments and longer-term structural strategies to cope with thermal stress, with mechanistic traits facilitating rapid adjustments and functional traits maintaining ecological stability.
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spelling doaj-art-cda3ccb351f147c8aaf7b5234b828c132025-08-20T02:42:25ZengMDPI AGPlants2223-77472025-03-0114696010.3390/plants14060960Plastic Responses of <i>Iris pumila</i> Functional and Mechanistic Leaf Traits to Experimental WarmingKatarina Hočevar0Ana Vuleta1Sanja Manitašević Jovanović2Department of Evolutionary Biology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, 11108 Belgrade, SerbiaDepartment of Evolutionary Biology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, 11108 Belgrade, SerbiaDepartment of Evolutionary Biology, Institute for Biological Research “Siniša Stanković”—National Institute of the Republic of Serbia, University of Belgrade, 11108 Belgrade, SerbiaPhenotypic plasticity is an important adaptive strategy that enables plants to respond to environmental changes, particularly temperature fluctuations associated with global warming. In this study, the phenotypic plasticity of <i data-eusoft-scrollable-element="1">Iris pumila</i> leaf traits in response to an elevated temperature (by 1 °C) was investigated under controlled experimental conditions. In particular, we investigated important functional and mechanistic leaf traits: specific leaf area (SLA), leaf dry matter content (LDMC), specific leaf water content (SLWC), stomatal density (SD), leaf thickness (LT), and chlorophyll content. The results revealed that an elevated temperature induced trait-specific plastic responses, with mechanistic traits exhibiting greater plasticity than functional traits, reflecting their role in short-term acclimation. SLA and SD increased at higher temperatures, promoting photosynthesis and gas exchange, while reductions in SLWC, LDMC, LT, and chlorophyll content suggest a trade-off in favor of growth and metabolic activity over structural investment. Notably, chlorophyll content exhibited the highest plasticity, emphasizing its crucial role in modulating photosynthetic efficiency under thermal stress. Correlation analyses revealed strong phenotypic integration between leaf traits, with distinct trait relationships emerging under different temperature conditions. These findings suggest that <i data-eusoft-scrollable-element="1">I. pumila</i> employs both rapid physiological adjustments and longer-term structural strategies to cope with thermal stress, with mechanistic traits facilitating rapid adjustments and functional traits maintaining ecological stability.https://www.mdpi.com/2223-7747/14/6/960experimental warmingthermal acclimationphenotypic plasticityfunctional leaf traitsmechanistic leaf traits<i>Iris pumila</i> L.
spellingShingle Katarina Hočevar
Ana Vuleta
Sanja Manitašević Jovanović
Plastic Responses of <i>Iris pumila</i> Functional and Mechanistic Leaf Traits to Experimental Warming
Plants
experimental warming
thermal acclimation
phenotypic plasticity
functional leaf traits
mechanistic leaf traits
<i>Iris pumila</i> L.
title Plastic Responses of <i>Iris pumila</i> Functional and Mechanistic Leaf Traits to Experimental Warming
title_full Plastic Responses of <i>Iris pumila</i> Functional and Mechanistic Leaf Traits to Experimental Warming
title_fullStr Plastic Responses of <i>Iris pumila</i> Functional and Mechanistic Leaf Traits to Experimental Warming
title_full_unstemmed Plastic Responses of <i>Iris pumila</i> Functional and Mechanistic Leaf Traits to Experimental Warming
title_short Plastic Responses of <i>Iris pumila</i> Functional and Mechanistic Leaf Traits to Experimental Warming
title_sort plastic responses of i iris pumila i functional and mechanistic leaf traits to experimental warming
topic experimental warming
thermal acclimation
phenotypic plasticity
functional leaf traits
mechanistic leaf traits
<i>Iris pumila</i> L.
url https://www.mdpi.com/2223-7747/14/6/960
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AT sanjamanitasevicjovanovic plasticresponsesofiirispumilaifunctionalandmechanisticleaftraitstoexperimentalwarming