Spread of TEM, VIM, SHV, and CTX-M β-Lactamases in Imipenem-Resistant Gram-Negative Bacilli Isolated from Egyptian Hospitals
Carbapenem-resistant Gram-negative bacilli resulting from β-lactamases have been reported to be an important cause of nosocomial infections and are a critical therapeutic problem worldwide. This study aimed to describe the prevalence of imipenem-resistant Gram-negative bacilli isolates and detection...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2016/8382605 |
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| author | El sayed Hamdy Mohammed Ahmed Elsadek Fakhr Hanan Mohammed El sayed Said abd Elmohsen Al Johery Wesam Abdel Ghani Hassanein |
| author_facet | El sayed Hamdy Mohammed Ahmed Elsadek Fakhr Hanan Mohammed El sayed Said abd Elmohsen Al Johery Wesam Abdel Ghani Hassanein |
| author_sort | El sayed Hamdy Mohammed |
| collection | DOAJ |
| description | Carbapenem-resistant Gram-negative bacilli resulting from β-lactamases have been reported to be an important cause of nosocomial infections and are a critical therapeutic problem worldwide. This study aimed to describe the prevalence of imipenem-resistant Gram-negative bacilli isolates and detection of blaVIM, blaTEM, blaSHV, blaCTX-M-1, and blaCTX-M-9 genes in these clinical isolates in Egyptian hospitals. The isolates were collected from various clinical samples, identified by conventional methods and confirmed by API 20E. Antibiotic susceptibility testing was determined by Kirby-Bauer technique and interpreted according to CLSI. Production of blaVIM, blaTEM, blaSHV, and blaCTX-M genes was done by polymerase chain reaction (PCR). Direct sequencing from PCR products was subsequently carried out to identify and confirm these β-lactamases genes. Out of 65 isolates, (46.1%) Escherichia coli, (26.2%) Klebsiella pneumoniae, and (10.7%) Pseudomonas aeruginosa were identified as the commonest Gram-negative bacilli. 33(50.8%) were imipenem-resistant isolates. 22 isolates (66.7%) carried blaVIM, 24(72.7%) had blaTEM, and 5(15%) showed blaSHV, while 12(36%), 6(18.2%), and 0(0.00%) harbored blaCTX-M-1, blaCTX-M-9, and blaCTX-M-8/25, respectively. There is a high occurrence of β-lactamase genes in clinical isolates and sequence analysis of amplified genes showed differences between multiple SNPs (single nucleotide polymorphism) sites in the same gene among local isolates in relation to published sequences. |
| format | Article |
| id | doaj-art-cda3840604544ff4832ebee059bc2702 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-cda3840604544ff4832ebee059bc27022025-02-03T05:44:37ZengWileyInternational Journal of Microbiology1687-918X1687-91982016-01-01201610.1155/2016/83826058382605Spread of TEM, VIM, SHV, and CTX-M β-Lactamases in Imipenem-Resistant Gram-Negative Bacilli Isolated from Egyptian HospitalsEl sayed Hamdy Mohammed0Ahmed Elsadek Fakhr1Hanan Mohammed El sayed2Said abd Elmohsen Al Johery3Wesam Abdel Ghani Hassanein4Department of Botany, Faculty of Science, Zagazig University, Zagazig 44511, EgyptDepartment of Medical Microbiology & Immunology, Faculty of Medicine, Zagazig University, Zagazig 44511, EgyptDepartment of Medical Microbiology & Immunology, Faculty of Medicine, Zagazig University, Zagazig 44511, EgyptDepartment of Medical Microbiology & Immunology, Faculty of Medicine, Zagazig University, Zagazig 44511, EgyptDepartment of Botany, Faculty of Science, Zagazig University, Zagazig 44511, EgyptCarbapenem-resistant Gram-negative bacilli resulting from β-lactamases have been reported to be an important cause of nosocomial infections and are a critical therapeutic problem worldwide. This study aimed to describe the prevalence of imipenem-resistant Gram-negative bacilli isolates and detection of blaVIM, blaTEM, blaSHV, blaCTX-M-1, and blaCTX-M-9 genes in these clinical isolates in Egyptian hospitals. The isolates were collected from various clinical samples, identified by conventional methods and confirmed by API 20E. Antibiotic susceptibility testing was determined by Kirby-Bauer technique and interpreted according to CLSI. Production of blaVIM, blaTEM, blaSHV, and blaCTX-M genes was done by polymerase chain reaction (PCR). Direct sequencing from PCR products was subsequently carried out to identify and confirm these β-lactamases genes. Out of 65 isolates, (46.1%) Escherichia coli, (26.2%) Klebsiella pneumoniae, and (10.7%) Pseudomonas aeruginosa were identified as the commonest Gram-negative bacilli. 33(50.8%) were imipenem-resistant isolates. 22 isolates (66.7%) carried blaVIM, 24(72.7%) had blaTEM, and 5(15%) showed blaSHV, while 12(36%), 6(18.2%), and 0(0.00%) harbored blaCTX-M-1, blaCTX-M-9, and blaCTX-M-8/25, respectively. There is a high occurrence of β-lactamase genes in clinical isolates and sequence analysis of amplified genes showed differences between multiple SNPs (single nucleotide polymorphism) sites in the same gene among local isolates in relation to published sequences.http://dx.doi.org/10.1155/2016/8382605 |
| spellingShingle | El sayed Hamdy Mohammed Ahmed Elsadek Fakhr Hanan Mohammed El sayed Said abd Elmohsen Al Johery Wesam Abdel Ghani Hassanein Spread of TEM, VIM, SHV, and CTX-M β-Lactamases in Imipenem-Resistant Gram-Negative Bacilli Isolated from Egyptian Hospitals International Journal of Microbiology |
| title | Spread of TEM, VIM, SHV, and CTX-M β-Lactamases in Imipenem-Resistant Gram-Negative Bacilli Isolated from Egyptian Hospitals |
| title_full | Spread of TEM, VIM, SHV, and CTX-M β-Lactamases in Imipenem-Resistant Gram-Negative Bacilli Isolated from Egyptian Hospitals |
| title_fullStr | Spread of TEM, VIM, SHV, and CTX-M β-Lactamases in Imipenem-Resistant Gram-Negative Bacilli Isolated from Egyptian Hospitals |
| title_full_unstemmed | Spread of TEM, VIM, SHV, and CTX-M β-Lactamases in Imipenem-Resistant Gram-Negative Bacilli Isolated from Egyptian Hospitals |
| title_short | Spread of TEM, VIM, SHV, and CTX-M β-Lactamases in Imipenem-Resistant Gram-Negative Bacilli Isolated from Egyptian Hospitals |
| title_sort | spread of tem vim shv and ctx m β lactamases in imipenem resistant gram negative bacilli isolated from egyptian hospitals |
| url | http://dx.doi.org/10.1155/2016/8382605 |
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