A Pipette for High-Resolution Sampling and Delivery of pL Bio-Samples
Conventional laboratory methods for handling valuable biological samples typically use pipettes or needles, which are prone to issues such as dead volume and sample waste. Furthermore, the sampling and processing of pathogenic bacteria, such as <i>Escherichia coli</i> (<i>E. coli&l...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-05-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/16/6/630 |
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| author | Ziyang Han Pengfei Gong Hengxiang Su Zehang Gao Shilun Feng Jianlong Zhao |
| author_facet | Ziyang Han Pengfei Gong Hengxiang Su Zehang Gao Shilun Feng Jianlong Zhao |
| author_sort | Ziyang Han |
| collection | DOAJ |
| description | Conventional laboratory methods for handling valuable biological samples typically use pipettes or needles, which are prone to issues such as dead volume and sample waste. Furthermore, the sampling and processing of pathogenic bacteria, such as <i>Escherichia coli</i> (<i>E. coli</i>) in environmental wastewater, require labor-intensive procedures with multiple complex steps. To overcome these limitations, we developed a pipette integrated with a microfluidic chip for bacteria sampling and delivery. This pipette can provide the negative pressure to microfluidic chips for sampling, the constant temperature unit for biological reaction, and programs for automatic control (suction, heating, liquid discharge, and cleaning) and display. The droplet chip employs a cross-channel structure to generate droplets and incorporates a designated droplet storage and detection area. Utilizing this innovative device, we have demonstrated the generation, transportation, and storage of pL droplets, as well as quantitatively detected <i>E. coli</i> samples across various concentrations. The test results have demonstrated the overall reliability and data consistency of the system. Overall, our device achieves the precise sampling of pL volumes, offering a simple yet promising solution for the sampling and delivery of bio-samples in remote settings. |
| format | Article |
| id | doaj-art-cd98473c2343409c9ad9d4e547d023cc |
| institution | Kabale University |
| issn | 2072-666X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj-art-cd98473c2343409c9ad9d4e547d023cc2025-08-20T03:29:44ZengMDPI AGMicromachines2072-666X2025-05-0116663010.3390/mi16060630A Pipette for High-Resolution Sampling and Delivery of pL Bio-SamplesZiyang Han0Pengfei Gong1Hengxiang Su2Zehang Gao3Shilun Feng4Jianlong Zhao5State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, ChinaState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, ChinaXiangfu Laboratory, Jiashan 314102, ChinaState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, ChinaState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, ChinaState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, ChinaConventional laboratory methods for handling valuable biological samples typically use pipettes or needles, which are prone to issues such as dead volume and sample waste. Furthermore, the sampling and processing of pathogenic bacteria, such as <i>Escherichia coli</i> (<i>E. coli</i>) in environmental wastewater, require labor-intensive procedures with multiple complex steps. To overcome these limitations, we developed a pipette integrated with a microfluidic chip for bacteria sampling and delivery. This pipette can provide the negative pressure to microfluidic chips for sampling, the constant temperature unit for biological reaction, and programs for automatic control (suction, heating, liquid discharge, and cleaning) and display. The droplet chip employs a cross-channel structure to generate droplets and incorporates a designated droplet storage and detection area. Utilizing this innovative device, we have demonstrated the generation, transportation, and storage of pL droplets, as well as quantitatively detected <i>E. coli</i> samples across various concentrations. The test results have demonstrated the overall reliability and data consistency of the system. Overall, our device achieves the precise sampling of pL volumes, offering a simple yet promising solution for the sampling and delivery of bio-samples in remote settings.https://www.mdpi.com/2072-666X/16/6/630sampling pipettebiological detectionmicrofluidic systemmicrodroplet<i>E. coli</i> |
| spellingShingle | Ziyang Han Pengfei Gong Hengxiang Su Zehang Gao Shilun Feng Jianlong Zhao A Pipette for High-Resolution Sampling and Delivery of pL Bio-Samples Micromachines sampling pipette biological detection microfluidic system microdroplet <i>E. coli</i> |
| title | A Pipette for High-Resolution Sampling and Delivery of pL Bio-Samples |
| title_full | A Pipette for High-Resolution Sampling and Delivery of pL Bio-Samples |
| title_fullStr | A Pipette for High-Resolution Sampling and Delivery of pL Bio-Samples |
| title_full_unstemmed | A Pipette for High-Resolution Sampling and Delivery of pL Bio-Samples |
| title_short | A Pipette for High-Resolution Sampling and Delivery of pL Bio-Samples |
| title_sort | pipette for high resolution sampling and delivery of pl bio samples |
| topic | sampling pipette biological detection microfluidic system microdroplet <i>E. coli</i> |
| url | https://www.mdpi.com/2072-666X/16/6/630 |
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