RETRACTED: Circ_0087199 depletion attenuates lipopolysaccharides‐induced human periodontal ligament cell injury through the miR‐527/TLR4 axis
Abstract Background Circular RNAs participate in the development of periodontitis. The present work aims to reveal the role and mechanism of circ_0087199 in human periodontal ligament cell (PDLC) injury during periodontitis. Methods PDLCs were treated with lipopolysaccharides (LPS) to establish a pe...
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| Format: | Article |
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Wiley
2024-01-01
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| Series: | Immunity, Inflammation and Disease |
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| Online Access: | https://doi.org/10.1002/iid3.1153 |
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| author | Jing Wang Na Huo Chuan Cai Yu Zhang Rui Xiao |
| author_facet | Jing Wang Na Huo Chuan Cai Yu Zhang Rui Xiao |
| author_sort | Jing Wang |
| collection | DOAJ |
| description | Abstract Background Circular RNAs participate in the development of periodontitis. The present work aims to reveal the role and mechanism of circ_0087199 in human periodontal ligament cell (PDLC) injury during periodontitis. Methods PDLCs were treated with lipopolysaccharides (LPS) to establish a periodontitis cell model. Quantitative real‐time polymerase chain reaction was used to detect the expression of circ_0087199, miR‐527, toll‐like receptor 4 (TLR4). Western blot analysis assay was performed to assess protein expression. Cell viability, proliferation, apoptosis and inflammation were investigated by cell counting kit‐8, EdU assay, flow cytometry and enzyme‐linked immunosorbent assay, respectively. Oxidative stress was evaluated by malondialdehyde assay kit and superoxide dismutase activity assay kit. The interaction between miR‐527 and circ_0087199 or TLR4 was confirmed by a dual‐luciferase reporter assay. Results Circ_0087199 and TLR4 expression levels were significantly increased, while miR‐527 was decreased in the periodontal ligament tissues of periodontitis patients and LPS‐stimulated PDLCs when compared with controls. LPS treatment inhibited cell viability and proliferation but induced cell apoptosis, inflammation and oxidative stress, whereas these effects were attenuated after circ_0087199 knockdown. Circ_0087199 bound to miR‐527 and regulated LPS‐caused PDLC damage by targeting miR‐527. Additionally, the overexpression of TLR4, a target gene of miR‐527, rescued miR‐527 mimic‐mediated effects on LPS‐treated PDLCs. Further, the regulation of circ_0087199 toward TLR4 involved miR‐527. Conclusion Circ_0087199 knockdown attenuated LPS‐induced apoptosis, inflammation and oxidative stress of PDLCs by regulating the miR‐527/TLR4 pathway. |
| format | Article |
| id | doaj-art-cd91fdb0018648b8be7d021bf4d81b7a |
| institution | OA Journals |
| issn | 2050-4527 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
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| series | Immunity, Inflammation and Disease |
| spelling | doaj-art-cd91fdb0018648b8be7d021bf4d81b7a2025-08-20T02:35:08ZengWileyImmunity, Inflammation and Disease2050-45272024-01-01121n/an/a10.1002/iid3.1153RETRACTED: Circ_0087199 depletion attenuates lipopolysaccharides‐induced human periodontal ligament cell injury through the miR‐527/TLR4 axisJing Wang0Na Huo1Chuan Cai2Yu Zhang3Rui Xiao4Department of Stomatolory 1st Medical Center of PLA General Hospital Beijing ChinaDepartment of Stomatolory 1st Medical Center of PLA General Hospital Beijing ChinaDepartment of Stomatolory 1st Medical Center of PLA General Hospital Beijing ChinaDepartment of Stomatolory 1st Medical Center of PLA General Hospital Beijing ChinaDepartment of Stomatolory 1st Medical Center of PLA General Hospital Beijing ChinaAbstract Background Circular RNAs participate in the development of periodontitis. The present work aims to reveal the role and mechanism of circ_0087199 in human periodontal ligament cell (PDLC) injury during periodontitis. Methods PDLCs were treated with lipopolysaccharides (LPS) to establish a periodontitis cell model. Quantitative real‐time polymerase chain reaction was used to detect the expression of circ_0087199, miR‐527, toll‐like receptor 4 (TLR4). Western blot analysis assay was performed to assess protein expression. Cell viability, proliferation, apoptosis and inflammation were investigated by cell counting kit‐8, EdU assay, flow cytometry and enzyme‐linked immunosorbent assay, respectively. Oxidative stress was evaluated by malondialdehyde assay kit and superoxide dismutase activity assay kit. The interaction between miR‐527 and circ_0087199 or TLR4 was confirmed by a dual‐luciferase reporter assay. Results Circ_0087199 and TLR4 expression levels were significantly increased, while miR‐527 was decreased in the periodontal ligament tissues of periodontitis patients and LPS‐stimulated PDLCs when compared with controls. LPS treatment inhibited cell viability and proliferation but induced cell apoptosis, inflammation and oxidative stress, whereas these effects were attenuated after circ_0087199 knockdown. Circ_0087199 bound to miR‐527 and regulated LPS‐caused PDLC damage by targeting miR‐527. Additionally, the overexpression of TLR4, a target gene of miR‐527, rescued miR‐527 mimic‐mediated effects on LPS‐treated PDLCs. Further, the regulation of circ_0087199 toward TLR4 involved miR‐527. Conclusion Circ_0087199 knockdown attenuated LPS‐induced apoptosis, inflammation and oxidative stress of PDLCs by regulating the miR‐527/TLR4 pathway.https://doi.org/10.1002/iid3.1153circ_0087199PDLCsperiodontitismiR‐527TLR4 |
| spellingShingle | Jing Wang Na Huo Chuan Cai Yu Zhang Rui Xiao RETRACTED: Circ_0087199 depletion attenuates lipopolysaccharides‐induced human periodontal ligament cell injury through the miR‐527/TLR4 axis Immunity, Inflammation and Disease circ_0087199 PDLCs periodontitis miR‐527 TLR4 |
| title | RETRACTED: Circ_0087199 depletion attenuates lipopolysaccharides‐induced human periodontal ligament cell injury through the miR‐527/TLR4 axis |
| title_full | RETRACTED: Circ_0087199 depletion attenuates lipopolysaccharides‐induced human periodontal ligament cell injury through the miR‐527/TLR4 axis |
| title_fullStr | RETRACTED: Circ_0087199 depletion attenuates lipopolysaccharides‐induced human periodontal ligament cell injury through the miR‐527/TLR4 axis |
| title_full_unstemmed | RETRACTED: Circ_0087199 depletion attenuates lipopolysaccharides‐induced human periodontal ligament cell injury through the miR‐527/TLR4 axis |
| title_short | RETRACTED: Circ_0087199 depletion attenuates lipopolysaccharides‐induced human periodontal ligament cell injury through the miR‐527/TLR4 axis |
| title_sort | retracted circ 0087199 depletion attenuates lipopolysaccharides induced human periodontal ligament cell injury through the mir 527 tlr4 axis |
| topic | circ_0087199 PDLCs periodontitis miR‐527 TLR4 |
| url | https://doi.org/10.1002/iid3.1153 |
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