Establishment of a mouse lung cancer organoid model and its applications for therapeutic screening
Abstract Background Lung cancer exhibits a high degree of heterogeneity, rendering its treatment one of the most difficult challenges. Current laboratory lung cancer models cannot fully preserve the diversity of the disease and predict drug responses. Owing to the rarity of human-derived samples, tr...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-06-01
|
| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12575-025-00284-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850114519357980672 |
|---|---|
| author | Yajing Liu Jingyuan Ning Donglai Wang |
| author_facet | Yajing Liu Jingyuan Ning Donglai Wang |
| author_sort | Yajing Liu |
| collection | DOAJ |
| description | Abstract Background Lung cancer exhibits a high degree of heterogeneity, rendering its treatment one of the most difficult challenges. Current laboratory lung cancer models cannot fully preserve the diversity of the disease and predict drug responses. Owing to the rarity of human-derived samples, transgenic mouse models can be extensively used in fundamental research. Results We established a mouse lung cancer organoid (LCO) model derived from a genetically engineered mouse model that can spontaneously develop lung cancer. Morphological and molecular assays demonstrated that the lung cancer organoids retained the histological architecture and stem-like characteristics of their parental tumors. Successfully constructed lung cancer organoids were amenable for high-throughput drug screening in vitro. Conclusions This method can be used for the construction and identification of mouse-derived lung cancer organoids, which can be used to further comprehend the pathophysiology of lung cancer and evaluate drug responses in personalized medicine. |
| format | Article |
| id | doaj-art-cd84882a7fe54786800fe72d3e47ba41 |
| institution | OA Journals |
| issn | 1480-9222 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj-art-cd84882a7fe54786800fe72d3e47ba412025-08-20T02:36:50ZengBMCBiological Procedures Online1480-92222025-06-0127111210.1186/s12575-025-00284-3Establishment of a mouse lung cancer organoid model and its applications for therapeutic screeningYajing Liu0Jingyuan Ning1Donglai Wang2State Key Laboratory of Common Mechanism Research for Major Diseases & Department of Medical Genetics, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical CollegeState Key Laboratory of Common Mechanism Research for Major Diseases & Department of Medical Genetics, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical CollegeState Key Laboratory of Common Mechanism Research for Major Diseases & Department of Medical Genetics, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical CollegeAbstract Background Lung cancer exhibits a high degree of heterogeneity, rendering its treatment one of the most difficult challenges. Current laboratory lung cancer models cannot fully preserve the diversity of the disease and predict drug responses. Owing to the rarity of human-derived samples, transgenic mouse models can be extensively used in fundamental research. Results We established a mouse lung cancer organoid (LCO) model derived from a genetically engineered mouse model that can spontaneously develop lung cancer. Morphological and molecular assays demonstrated that the lung cancer organoids retained the histological architecture and stem-like characteristics of their parental tumors. Successfully constructed lung cancer organoids were amenable for high-throughput drug screening in vitro. Conclusions This method can be used for the construction and identification of mouse-derived lung cancer organoids, which can be used to further comprehend the pathophysiology of lung cancer and evaluate drug responses in personalized medicine.https://doi.org/10.1186/s12575-025-00284-3Lung cancerOrganoidsKRASDrug screening |
| spellingShingle | Yajing Liu Jingyuan Ning Donglai Wang Establishment of a mouse lung cancer organoid model and its applications for therapeutic screening Biological Procedures Online Lung cancer Organoids KRAS Drug screening |
| title | Establishment of a mouse lung cancer organoid model and its applications for therapeutic screening |
| title_full | Establishment of a mouse lung cancer organoid model and its applications for therapeutic screening |
| title_fullStr | Establishment of a mouse lung cancer organoid model and its applications for therapeutic screening |
| title_full_unstemmed | Establishment of a mouse lung cancer organoid model and its applications for therapeutic screening |
| title_short | Establishment of a mouse lung cancer organoid model and its applications for therapeutic screening |
| title_sort | establishment of a mouse lung cancer organoid model and its applications for therapeutic screening |
| topic | Lung cancer Organoids KRAS Drug screening |
| url | https://doi.org/10.1186/s12575-025-00284-3 |
| work_keys_str_mv | AT yajingliu establishmentofamouselungcancerorganoidmodelanditsapplicationsfortherapeuticscreening AT jingyuanning establishmentofamouselungcancerorganoidmodelanditsapplicationsfortherapeuticscreening AT donglaiwang establishmentofamouselungcancerorganoidmodelanditsapplicationsfortherapeuticscreening |