Identification of SRAP molecular markers linked to powdery mildew resistance in cucumber
With a F<sub>2</sub> population between a resistant parent and susceptible parent, SRAP analysis was carried to develop molecular markers linked to powdery mildew in cucumber. Sixty-two pairs of primer combinations which had stable amplified polymorphic bands between resistant parent and...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2011-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.04.005 |
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| _version_ | 1849412785032658944 |
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| author | JING Ran CHEN Xin-juan ZHU Yu-qiang ZHANG Peng ZHANG Yu-qiong ZHOU Shengjun |
| author_facet | JING Ran CHEN Xin-juan ZHU Yu-qiang ZHANG Peng ZHANG Yu-qiong ZHOU Shengjun |
| author_sort | JING Ran |
| collection | DOAJ |
| description | With a F<sub>2</sub> population between a resistant parent and susceptible parent, SRAP analysis was carried to develop molecular markers linked to powdery mildew in cucumber. Sixty-two pairs of primer combinations which had stable amplified polymorphic bands between resistant parent and susceptible parent were selected from 437 pairs of SRAP primer combinations. Further analyzing the F<sub>2</sub> segregating populations using these primers, one SRAP marker of Me1 /Em9—284 bp linked to the powdery mildew resistance was found, with 9.8 cM genetic distance. The marker can be used for cloning disease-resistant gene and advancing efficiency of disease-resistant breeding on molecular marker assisted selection. |
| format | Article |
| id | doaj-art-cd7a806c7238478d80bc7adafbc7da2e |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2011-07-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-cd7a806c7238478d80bc7adafbc7da2e2025-08-20T03:34:20ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552011-07-013738739210.3785/j.issn.1008-9209.2011.04.00510089209Identification of SRAP molecular markers linked to powdery mildew resistance in cucumberJING RanCHEN Xin-juanZHU Yu-qiangZHANG PengZHANG Yu-qiongZHOU ShengjunWith a F<sub>2</sub> population between a resistant parent and susceptible parent, SRAP analysis was carried to develop molecular markers linked to powdery mildew in cucumber. Sixty-two pairs of primer combinations which had stable amplified polymorphic bands between resistant parent and susceptible parent were selected from 437 pairs of SRAP primer combinations. Further analyzing the F<sub>2</sub> segregating populations using these primers, one SRAP marker of Me1 /Em9—284 bp linked to the powdery mildew resistance was found, with 9.8 cM genetic distance. The marker can be used for cloning disease-resistant gene and advancing efficiency of disease-resistant breeding on molecular marker assisted selection.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.04.005cucumberpowdery mildewsequence-related amplified polymorphism (SRAP) marker |
| spellingShingle | JING Ran CHEN Xin-juan ZHU Yu-qiang ZHANG Peng ZHANG Yu-qiong ZHOU Shengjun Identification of SRAP molecular markers linked to powdery mildew resistance in cucumber 浙江大学学报. 农业与生命科学版 cucumber powdery mildew sequence-related amplified polymorphism (SRAP) marker |
| title | Identification of SRAP molecular markers linked to powdery mildew resistance in cucumber |
| title_full | Identification of SRAP molecular markers linked to powdery mildew resistance in cucumber |
| title_fullStr | Identification of SRAP molecular markers linked to powdery mildew resistance in cucumber |
| title_full_unstemmed | Identification of SRAP molecular markers linked to powdery mildew resistance in cucumber |
| title_short | Identification of SRAP molecular markers linked to powdery mildew resistance in cucumber |
| title_sort | identification of srap molecular markers linked to powdery mildew resistance in cucumber |
| topic | cucumber powdery mildew sequence-related amplified polymorphism (SRAP) marker |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.04.005 |
| work_keys_str_mv | AT jingran identificationofsrapmolecularmarkerslinkedtopowderymildewresistanceincucumber AT chenxinjuan identificationofsrapmolecularmarkerslinkedtopowderymildewresistanceincucumber AT zhuyuqiang identificationofsrapmolecularmarkerslinkedtopowderymildewresistanceincucumber AT zhangpeng identificationofsrapmolecularmarkerslinkedtopowderymildewresistanceincucumber AT zhangyuqiong identificationofsrapmolecularmarkerslinkedtopowderymildewresistanceincucumber AT zhoushengjun identificationofsrapmolecularmarkerslinkedtopowderymildewresistanceincucumber |