Identification of SRAP molecular markers linked to powdery mildew resistance in cucumber
With a F<sub>2</sub> population between a resistant parent and susceptible parent, SRAP analysis was carried to develop molecular markers linked to powdery mildew in cucumber. Sixty-two pairs of primer combinations which had stable amplified polymorphic bands between resistant parent and...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2011-07-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2011.04.005 |
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| Summary: | With a F<sub>2</sub> population between a resistant parent and susceptible parent, SRAP analysis was carried to develop molecular markers linked to powdery mildew in cucumber. Sixty-two pairs of primer combinations which had stable amplified polymorphic bands between resistant parent and susceptible parent were selected from 437 pairs of SRAP primer combinations. Further analyzing the F<sub>2</sub> segregating populations using these primers, one SRAP marker of Me1 /Em9—284 bp linked to the powdery mildew resistance was found, with 9.8 cM genetic distance. The marker can be used for cloning disease-resistant gene and advancing efficiency of disease-resistant breeding on molecular marker assisted selection. |
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| ISSN: | 1008-9209 2097-5155 |