Preparation and Structural Identification of Peptides Stimulating Cholecystokinin Secretion from the Skin of Puffer Fish
To obtain collagen peptides with the ability to stimulate cholecystokinin (CCK) secretion, collagen was extracted from puffer fish skin and hydrolyzed with alkaline protease. The CCK secretion-promoting activity of collagen peptides with different hydrolysis degrees was evaluated using an in vitro e...
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China Food Publishing Company
2025-06-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-12-018.pdf |
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| author | SONG Hongdong, REN Shaoxia, SHAO Zhuwei, GE Shaocheng, QIAO Kun, LIU Zhiyu |
| author_facet | SONG Hongdong, REN Shaoxia, SHAO Zhuwei, GE Shaocheng, QIAO Kun, LIU Zhiyu |
| author_sort | SONG Hongdong, REN Shaoxia, SHAO Zhuwei, GE Shaocheng, QIAO Kun, LIU Zhiyu |
| collection | DOAJ |
| description | To obtain collagen peptides with the ability to stimulate cholecystokinin (CCK) secretion, collagen was extracted from puffer fish skin and hydrolyzed with alkaline protease. The CCK secretion-promoting activity of collagen peptides with different hydrolysis degrees was evaluated using an in vitro enteroendocrine STC-1 cell model. The results showed that the peptide with a hydrolysis degree of 12% had the highest CCK secretion-promoting activity. This peptide was separated and purified using a YMC ODS C18 column. Six peptide sequences were identified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Among them, three peptides, LSGK, LPGKPGL and KMTPT, had significant CCK secretion-promoting activity. This study provides a theoretical basis for the application of puffer fish skin peptides in regulating intestinal CCK secretion and provides new ideas for the development of functional products based on puffer fish skin peptides. |
| format | Article |
| id | doaj-art-cd772bb4da2c43c8976f0b80d117eddf |
| institution | DOAJ |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-cd772bb4da2c43c8976f0b80d117eddf2025-08-20T03:12:32ZengChina Food Publishing CompanyShipin Kexue1002-66302025-06-01461215616210.7506/spkx1002-6630-20241111-075Preparation and Structural Identification of Peptides Stimulating Cholecystokinin Secretion from the Skin of Puffer FishSONG Hongdong, REN Shaoxia, SHAO Zhuwei, GE Shaocheng, QIAO Kun, LIU Zhiyu0(1. School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; 2. National R&D Center for Marine Fish Processing (Xiamen), Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China)To obtain collagen peptides with the ability to stimulate cholecystokinin (CCK) secretion, collagen was extracted from puffer fish skin and hydrolyzed with alkaline protease. The CCK secretion-promoting activity of collagen peptides with different hydrolysis degrees was evaluated using an in vitro enteroendocrine STC-1 cell model. The results showed that the peptide with a hydrolysis degree of 12% had the highest CCK secretion-promoting activity. This peptide was separated and purified using a YMC ODS C18 column. Six peptide sequences were identified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Among them, three peptides, LSGK, LPGKPGL and KMTPT, had significant CCK secretion-promoting activity. This study provides a theoretical basis for the application of puffer fish skin peptides in regulating intestinal CCK secretion and provides new ideas for the development of functional products based on puffer fish skin peptides.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-12-018.pdfpuffer fish skin; collagen peptide; cholecystokinin; stc-1 cells; separation and purification; structural identification |
| spellingShingle | SONG Hongdong, REN Shaoxia, SHAO Zhuwei, GE Shaocheng, QIAO Kun, LIU Zhiyu Preparation and Structural Identification of Peptides Stimulating Cholecystokinin Secretion from the Skin of Puffer Fish Shipin Kexue puffer fish skin; collagen peptide; cholecystokinin; stc-1 cells; separation and purification; structural identification |
| title | Preparation and Structural Identification of Peptides Stimulating Cholecystokinin Secretion from the Skin of Puffer Fish |
| title_full | Preparation and Structural Identification of Peptides Stimulating Cholecystokinin Secretion from the Skin of Puffer Fish |
| title_fullStr | Preparation and Structural Identification of Peptides Stimulating Cholecystokinin Secretion from the Skin of Puffer Fish |
| title_full_unstemmed | Preparation and Structural Identification of Peptides Stimulating Cholecystokinin Secretion from the Skin of Puffer Fish |
| title_short | Preparation and Structural Identification of Peptides Stimulating Cholecystokinin Secretion from the Skin of Puffer Fish |
| title_sort | preparation and structural identification of peptides stimulating cholecystokinin secretion from the skin of puffer fish |
| topic | puffer fish skin; collagen peptide; cholecystokinin; stc-1 cells; separation and purification; structural identification |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-12-018.pdf |
| work_keys_str_mv | AT songhongdongrenshaoxiashaozhuweigeshaochengqiaokunliuzhiyu preparationandstructuralidentificationofpeptidesstimulatingcholecystokininsecretionfromtheskinofpufferfish |