Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp. bulgaricus in skimmed milk
Bioremediation of organophosphorus pesticides in contaminated foodstuffs using probiotics has been increasingly under the spotlight in recent years, though the biodegradation mechanism and derived intermediate products remain unclear. This study aimed to help fill this knowledge gap and examined the...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250244 |
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| author | Yue Yang Wenxia Zhou Lingyu Yang Yilun Chen Dongxiao Sun-Waterhouse Dapeng Li |
| author_facet | Yue Yang Wenxia Zhou Lingyu Yang Yilun Chen Dongxiao Sun-Waterhouse Dapeng Li |
| author_sort | Yue Yang |
| collection | DOAJ |
| description | Bioremediation of organophosphorus pesticides in contaminated foodstuffs using probiotics has been increasingly under the spotlight in recent years, though the biodegradation mechanism and derived intermediate products remain unclear. This study aimed to help fill this knowledge gap and examined the degradation mechanism of organophosphorus pesticide, chlorpyrifos, in milk by Lactobacillus delbrueckii ssp. bulgaricus using gas chromatography-tandem mass spectrometry (GC-MS/MS) combined with transcriptome analysis. After the strain was cultured for 20 h in the presence of chlorpyrifos, differential expressions of 383 genes were detected, including genes probably implicated during chlorpyrifos degradation such as those related to hydrolase, phosphoesterase, diphosphatase, oxidoreductase, dehydratase, as well as membrane transporters. GC-MS/MS analysis revealed the changes of secondary metabolites in L. bulgaricus during milk fermentation due to chlorpyrifos stress. 6-Methylhexahydro-2H-azepin-2-one, 2,6-dihydroxypyridine and methyl 2-aminooxy-4-methylpentanoate as intermediates, along with the proposed pathways, might be involved in chlorpyrifos biodegradation by L. bulgaricus. |
| format | Article |
| id | doaj-art-cd74c8ddf7fa48848750ac7ff26d6f58 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-cd74c8ddf7fa48848750ac7ff26d6f582025-01-10T06:56:56ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-09-011353018303010.26599/FSHW.2022.9250244Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp. bulgaricus in skimmed milkYue Yang0Wenxia Zhou1Lingyu Yang2Yilun Chen3Dongxiao Sun-Waterhouse4Dapeng Li5Key Laboratory of Food Nutrition and Human Health in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaKey Laboratory of Food Nutrition and Human Health in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaKey Laboratory of Food Nutrition and Human Health in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaKey Laboratory of Food Nutrition and Human Health in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaKey Laboratory of Food Nutrition and Human Health in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaKey Laboratory of Food Nutrition and Human Health in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaBioremediation of organophosphorus pesticides in contaminated foodstuffs using probiotics has been increasingly under the spotlight in recent years, though the biodegradation mechanism and derived intermediate products remain unclear. This study aimed to help fill this knowledge gap and examined the degradation mechanism of organophosphorus pesticide, chlorpyrifos, in milk by Lactobacillus delbrueckii ssp. bulgaricus using gas chromatography-tandem mass spectrometry (GC-MS/MS) combined with transcriptome analysis. After the strain was cultured for 20 h in the presence of chlorpyrifos, differential expressions of 383 genes were detected, including genes probably implicated during chlorpyrifos degradation such as those related to hydrolase, phosphoesterase, diphosphatase, oxidoreductase, dehydratase, as well as membrane transporters. GC-MS/MS analysis revealed the changes of secondary metabolites in L. bulgaricus during milk fermentation due to chlorpyrifos stress. 6-Methylhexahydro-2H-azepin-2-one, 2,6-dihydroxypyridine and methyl 2-aminooxy-4-methylpentanoate as intermediates, along with the proposed pathways, might be involved in chlorpyrifos biodegradation by L. bulgaricus.https://www.sciopen.com/article/10.26599/FSHW.2022.9250244lactobacillus delbrueckii ssp. bulgaricusbiodegradationchlorpyrifostranscriptomicsmetabolome |
| spellingShingle | Yue Yang Wenxia Zhou Lingyu Yang Yilun Chen Dongxiao Sun-Waterhouse Dapeng Li Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp. bulgaricus in skimmed milk Food Science and Human Wellness lactobacillus delbrueckii ssp. bulgaricus biodegradation chlorpyrifos transcriptomics metabolome |
| title | Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp. bulgaricus in skimmed milk |
| title_full | Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp. bulgaricus in skimmed milk |
| title_fullStr | Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp. bulgaricus in skimmed milk |
| title_full_unstemmed | Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp. bulgaricus in skimmed milk |
| title_short | Transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by Lactobacillus delbrueckii ssp. bulgaricus in skimmed milk |
| title_sort | transcriptomic analysis of molecular mechanisms underlying the biodegradation of organophosphorus pesticide chlorpyrifos by lactobacillus delbrueckii ssp bulgaricus in skimmed milk |
| topic | lactobacillus delbrueckii ssp. bulgaricus biodegradation chlorpyrifos transcriptomics metabolome |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250244 |
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