Reference values of lymphocyte subsets from healthy Polish adults

The flow cytometry method could support physicians’ decisions in the diagnosis and treatment monitoring of immunodeficient patients. Most clinical recommendations are focused on the search for alterations in T- and B-lymphocyte subsets, less commonly natural killer (NK) cells and granulocytes. While...

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Main Authors: Aleksander Roszczyk, Michał Zych, Dariusz Sołdacki, Radoslaw Zagozdzon, Monika J. Kniotek
Format: Article
Language:English
Published: Termedia Publishing House 2024-03-01
Series:Central European Journal of Immunology
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Online Access:https://www.termedia.pl/Reference-values-of-lymphocyte-subsets-from-healthy-Polish-adults,10,52655,1,1.html
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author Aleksander Roszczyk
Michał Zych
Dariusz Sołdacki
Radoslaw Zagozdzon
Monika J. Kniotek
author_facet Aleksander Roszczyk
Michał Zych
Dariusz Sołdacki
Radoslaw Zagozdzon
Monika J. Kniotek
author_sort Aleksander Roszczyk
collection DOAJ
description The flow cytometry method could support physicians’ decisions in the diagnosis and treatment monitoring of immunodeficient patients. Most clinical recommendations are focused on the search for alterations in T- and B-lymphocyte subsets, less commonly natural killer (NK) cells and granulocytes. While reference values for clinically meaningful lymphocyte subsets have been published ubiquitously among numerous countries, we have not found significant data for a population of adult Polish habitats; thus we determined reference values for T, B, and NK subsets according to sex and age. The female group showed a higher percentage of lymphocytes (CD45 ++ ), T helper lymphocytes with a higher absolute count, as well as CD4/CD8 ratio, marginal zone-like B cells, class-switched B cells, and CD21 low B cells than the male group. The male group was found to have elevated percentages of naïve B lymphocytes, transitional B cells, and plasmablasts. A weak positive correlation with age was found among double positive T lymphocytes, natural killer T cells (NKT) lymphocytes, and CD21 low B cells. A negative correlation with age for double negative T lymphocytes, marginal zone-like B cells, and plasmablasts was noted. The results indicated the importance of creating distinct reference ranges regarding sex and age concerning immunophenotype.
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issn 1426-3912
1644-4124
language English
publishDate 2024-03-01
publisher Termedia Publishing House
record_format Article
series Central European Journal of Immunology
spelling doaj-art-cd74b91efc7245b69dcfb15bbd2976bd2025-08-20T02:09:38ZengTermedia Publishing HouseCentral European Journal of Immunology1426-39121644-41242024-03-01491263610.5114/ceji.2024.13637152655Reference values of lymphocyte subsets from healthy Polish adultsAleksander RoszczykMichał ZychDariusz SołdackiRadoslaw ZagozdzonMonika J. KniotekThe flow cytometry method could support physicians’ decisions in the diagnosis and treatment monitoring of immunodeficient patients. Most clinical recommendations are focused on the search for alterations in T- and B-lymphocyte subsets, less commonly natural killer (NK) cells and granulocytes. While reference values for clinically meaningful lymphocyte subsets have been published ubiquitously among numerous countries, we have not found significant data for a population of adult Polish habitats; thus we determined reference values for T, B, and NK subsets according to sex and age. The female group showed a higher percentage of lymphocytes (CD45 ++ ), T helper lymphocytes with a higher absolute count, as well as CD4/CD8 ratio, marginal zone-like B cells, class-switched B cells, and CD21 low B cells than the male group. The male group was found to have elevated percentages of naïve B lymphocytes, transitional B cells, and plasmablasts. A weak positive correlation with age was found among double positive T lymphocytes, natural killer T cells (NKT) lymphocytes, and CD21 low B cells. A negative correlation with age for double negative T lymphocytes, marginal zone-like B cells, and plasmablasts was noted. The results indicated the importance of creating distinct reference ranges regarding sex and age concerning immunophenotype.https://www.termedia.pl/Reference-values-of-lymphocyte-subsets-from-healthy-Polish-adults,10,52655,1,1.htmlb lymphocytes subsets class-switching b lymphocytes cvid – common variable immunodeficiency flow cytometry healthy adults immunophenotype lymphocyte subsets peripheral blood nk – natural killer nkt – natural killer t cells primary immunodeficiency – pid reference values
spellingShingle Aleksander Roszczyk
Michał Zych
Dariusz Sołdacki
Radoslaw Zagozdzon
Monika J. Kniotek
Reference values of lymphocyte subsets from healthy Polish adults
Central European Journal of Immunology
b lymphocytes subsets
class-switching b lymphocytes
cvid – common variable immunodeficiency
flow cytometry
healthy adults
immunophenotype
lymphocyte subsets
peripheral blood
nk – natural killer
nkt – natural killer t cells
primary immunodeficiency – pid
reference values
title Reference values of lymphocyte subsets from healthy Polish adults
title_full Reference values of lymphocyte subsets from healthy Polish adults
title_fullStr Reference values of lymphocyte subsets from healthy Polish adults
title_full_unstemmed Reference values of lymphocyte subsets from healthy Polish adults
title_short Reference values of lymphocyte subsets from healthy Polish adults
title_sort reference values of lymphocyte subsets from healthy polish adults
topic b lymphocytes subsets
class-switching b lymphocytes
cvid – common variable immunodeficiency
flow cytometry
healthy adults
immunophenotype
lymphocyte subsets
peripheral blood
nk – natural killer
nkt – natural killer t cells
primary immunodeficiency – pid
reference values
url https://www.termedia.pl/Reference-values-of-lymphocyte-subsets-from-healthy-Polish-adults,10,52655,1,1.html
work_keys_str_mv AT aleksanderroszczyk referencevaluesoflymphocytesubsetsfromhealthypolishadults
AT michałzych referencevaluesoflymphocytesubsetsfromhealthypolishadults
AT dariuszsołdacki referencevaluesoflymphocytesubsetsfromhealthypolishadults
AT radoslawzagozdzon referencevaluesoflymphocytesubsetsfromhealthypolishadults
AT monikajkniotek referencevaluesoflymphocytesubsetsfromhealthypolishadults