Aseptic germination and bulblet formation of Ixiolirion tataricum (Pall.) Herb. seeds
Ixiolirion tataricum (Pall.) Herb. is a unique and elite plant germplasm resource in China. The distribution of I. tataricum is limited in Xinjiang Uygur Autonomous Region. Ixiolirion. tataricum has strong resistance, high ornamental and medicinal values, which are of great potential in breeding. In...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2019-06-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.07.041 |
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| Summary: | Ixiolirion tataricum (Pall.) Herb. is a unique and elite plant germplasm resource in China. The distribution of I. tataricum is limited in Xinjiang Uygur Autonomous Region. Ixiolirion. tataricum has strong resistance, high ornamental and medicinal values, which are of great potential in breeding. In this study, the seeds of I. tataricum collected from Xinjiang Uygur Autonomous Region were used as materials to compare the effects of sterilization method, culture conditions, gibberellin A<sub>3</sub> (GA<sub>3</sub>) pretreatment and storage conditions on the aseptic germination and bulblet formation. The results showed that 30 s treatment with 75% ethanol followed by 5 min treatment with 2% NaClO was the best sterilization method for I. tataricum with 100% sterile seedlings. Using this sterilization method, the germination rate was more than 94.0% at 4 ℃, while almost no seed germinated at 25 ℃. Normally, seeds could germinate after storage for six months in the refrigerator at 4 ℃, but completely lost germination capacity at room temperature. We also found that MS+6-benzylaminopurine (6-BA) 1.0 mg/L+1-naphthylacetic acid (NAA) 0.2 mg/L+sucrose 30 g/L was the best germination medium for I. tataricum, which was beneficial to bulblet formation. Germinated in this medium, shoots could eventually expanded into bulbs after being transferred to light culture at 25℃ and then transferred to MS medium containing 60 g/Lsucrose. This study provides an experimental basis for in vitro conservation of I. tataricum and to enhance the development and utilization of wild resources in China. |
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| ISSN: | 1008-9209 2097-5155 |