Neuroprotective effect of an unsaturated mannuronate oligosaccharide derived from enzyme-degraded polymannuronate on an in vitro Parkinson's disease-like model
This study mainly aimed to explore the intervention effect of an unsaturated mannuronate oligosaccharide (MOS) on Parkinson's disease (PD) and its potential mechanism at the cellular level. MOS, which possesses high water solubility and excellent biocompatibility, was prepared by enzyme degrada...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154325003655 |
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| author | Decheng Bi Jinfeng Huang Nanting Zhu Jue Cao Yan Wu Lijun Yao Xueying Ding Jianyong Wu Xu Cao Xu Xu |
| author_facet | Decheng Bi Jinfeng Huang Nanting Zhu Jue Cao Yan Wu Lijun Yao Xueying Ding Jianyong Wu Xu Cao Xu Xu |
| author_sort | Decheng Bi |
| collection | DOAJ |
| description | This study mainly aimed to explore the intervention effect of an unsaturated mannuronate oligosaccharide (MOS) on Parkinson's disease (PD) and its potential mechanism at the cellular level. MOS, which possesses high water solubility and excellent biocompatibility, was prepared by enzyme degradation of alginate-derived polymannuronate. In 6-hydroxydopamine (6-OHDA)-induced SH-SY5Y cell culture, MOS treatment effectively augmented the expression of tyrosine hydroxylase (TH), PTEN-induced putative kinase 1 (PINK1) and parkin but suppressed the expression of α-synuclein (α-syn). Moreover, MOS exerted antioxidant activity by suppressing the excessive generation of reactive oxygen species and enhancing the activity of superoxide dismutase. MOS also inhibited 6-OHDA-induced apoptosis by enhancing mitochondrial membrane potential and mitigating the Bax/Bcl-2 pathway and improving autophagy that was blocked by 6-OHDA. In summary, these results have demonstrated the significant neuroprotective effect of MOS on SH-SY5Y cells, indicating that MOS has a certain intervention effect on the development of PD. This provides a promising foundation for developing novel therapeutic strategies targeting neurodegenerative disorders. |
| format | Article |
| id | doaj-art-cd6d0c76458a45f497498a2428ea3bf3 |
| institution | Kabale University |
| issn | 2666-1543 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-cd6d0c76458a45f497498a2428ea3bf32025-08-20T03:32:37ZengElsevierJournal of Agriculture and Food Research2666-15432025-08-012210199410.1016/j.jafr.2025.101994Neuroprotective effect of an unsaturated mannuronate oligosaccharide derived from enzyme-degraded polymannuronate on an in vitro Parkinson's disease-like modelDecheng Bi0Jinfeng Huang1Nanting Zhu2Jue Cao3Yan Wu4Lijun Yao5Xueying Ding6Jianyong Wu7Xu Cao8Xu Xu9Shenzhen Key Laboratory of Marine Bioresources and Ecology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, PR ChinaShenzhen Key Laboratory of Marine Bioresources and Ecology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, PR China; Digestive Diseases Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, 518107, PR ChinaShenzhen Key Laboratory of Marine Bioresources and Ecology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, PR ChinaShenzhen Key Laboratory of Marine Bioresources and Ecology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, PR China; Key Laboratory Marine Biological Waste and Comprehensive Utilization of Guangdong Province, Zhanjiang, 524051, PR ChinaInstrumental Analysis Center, Shenzhen University, Shenzhen, 518060, PR ChinaShenzhen Key Laboratory of Marine Bioresources and Ecology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, PR ChinaDepartment of Neurology, Shenzhen University General Hospital, Shenzhen University Clinical Medical Academy, Shenzhen, 518055, PR ChinaDepartment of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong KongDepartment of Neurology, Shenzhen University General Hospital, Shenzhen University Clinical Medical Academy, Shenzhen, 518055, PR China; Corresponding author.Shenzhen Key Laboratory of Marine Bioresources and Ecology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, PR China; Corresponding author.This study mainly aimed to explore the intervention effect of an unsaturated mannuronate oligosaccharide (MOS) on Parkinson's disease (PD) and its potential mechanism at the cellular level. MOS, which possesses high water solubility and excellent biocompatibility, was prepared by enzyme degradation of alginate-derived polymannuronate. In 6-hydroxydopamine (6-OHDA)-induced SH-SY5Y cell culture, MOS treatment effectively augmented the expression of tyrosine hydroxylase (TH), PTEN-induced putative kinase 1 (PINK1) and parkin but suppressed the expression of α-synuclein (α-syn). Moreover, MOS exerted antioxidant activity by suppressing the excessive generation of reactive oxygen species and enhancing the activity of superoxide dismutase. MOS also inhibited 6-OHDA-induced apoptosis by enhancing mitochondrial membrane potential and mitigating the Bax/Bcl-2 pathway and improving autophagy that was blocked by 6-OHDA. In summary, these results have demonstrated the significant neuroprotective effect of MOS on SH-SY5Y cells, indicating that MOS has a certain intervention effect on the development of PD. This provides a promising foundation for developing novel therapeutic strategies targeting neurodegenerative disorders.http://www.sciencedirect.com/science/article/pii/S2666154325003655Unsaturated mannuronate oligosaccharide (MOS)Parkinson's disease (PD)Cell culture modelAntioxidantAnti-apoptosisAutophagy |
| spellingShingle | Decheng Bi Jinfeng Huang Nanting Zhu Jue Cao Yan Wu Lijun Yao Xueying Ding Jianyong Wu Xu Cao Xu Xu Neuroprotective effect of an unsaturated mannuronate oligosaccharide derived from enzyme-degraded polymannuronate on an in vitro Parkinson's disease-like model Journal of Agriculture and Food Research Unsaturated mannuronate oligosaccharide (MOS) Parkinson's disease (PD) Cell culture model Antioxidant Anti-apoptosis Autophagy |
| title | Neuroprotective effect of an unsaturated mannuronate oligosaccharide derived from enzyme-degraded polymannuronate on an in vitro Parkinson's disease-like model |
| title_full | Neuroprotective effect of an unsaturated mannuronate oligosaccharide derived from enzyme-degraded polymannuronate on an in vitro Parkinson's disease-like model |
| title_fullStr | Neuroprotective effect of an unsaturated mannuronate oligosaccharide derived from enzyme-degraded polymannuronate on an in vitro Parkinson's disease-like model |
| title_full_unstemmed | Neuroprotective effect of an unsaturated mannuronate oligosaccharide derived from enzyme-degraded polymannuronate on an in vitro Parkinson's disease-like model |
| title_short | Neuroprotective effect of an unsaturated mannuronate oligosaccharide derived from enzyme-degraded polymannuronate on an in vitro Parkinson's disease-like model |
| title_sort | neuroprotective effect of an unsaturated mannuronate oligosaccharide derived from enzyme degraded polymannuronate on an in vitro parkinson s disease like model |
| topic | Unsaturated mannuronate oligosaccharide (MOS) Parkinson's disease (PD) Cell culture model Antioxidant Anti-apoptosis Autophagy |
| url | http://www.sciencedirect.com/science/article/pii/S2666154325003655 |
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