Tocopherol and Tocotrienol Content in the Leaves of the Genus <i>Hypericum</i>: Impact of Species and Drying Technique

α-Tocopherol (α-T) predominates in photosynthetic tissues, while tocotrienols (T3s) are reported very rarely. The genus <i>Hypericum</i> stands out as one of the few exceptions. Given the potential health benefits associated with tocotrienols, sourcing them from natural origins is of int...

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Main Authors: Ieva Miķelsone, Elise Sipeniece, Dalija Segliņa, Paweł Górnaś
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/7/1079
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author Ieva Miķelsone
Elise Sipeniece
Dalija Segliņa
Paweł Górnaś
author_facet Ieva Miķelsone
Elise Sipeniece
Dalija Segliņa
Paweł Górnaś
author_sort Ieva Miķelsone
collection DOAJ
description α-Tocopherol (α-T) predominates in photosynthetic tissues, while tocotrienols (T3s) are reported very rarely. The genus <i>Hypericum</i> stands out as one of the few exceptions. Given the potential health benefits associated with tocotrienols, sourcing them from natural origins is of interest. The proper selection of plant material and the drying conditions are crucial steps in this process. Therefore, in the present study, we investigated the effects of four different drying techniques (freeze-drying, microwave–vacuum-, infrared oven and air-drying) on the tocochromanol content in leaves of three <i>Hypericum</i> species: <i>H. androsaemum</i>, <i>H. pseudohenryi</i>, and <i>H. hookerianum</i> and one hybrid <i>H. × inodorum</i>. The total tocochromanol content in the freeze-dried leaves harvested in September was 68.1–150.6 mg/100 g dry weight. α-T constituted 66.7–85.9% (<i>w</i>/<i>w</i>), while tocotrienols constituted 13–32% (<i>w</i>/<i>w</i>). <i>H. pseudohenryi</i> was characterized by the lowest tocotrienol content, while <i>H. androsaemum</i> and <i>H. hookerianum</i> had the highest, with δ-T3 and γ-T3, respectively, being predominant. Tocotrienols were more stable during drying than α-T. The greatest decrease in α-T content was observed during air-drying in the presence of sunlight, with a 27% difference compared to the absence of sunlight. The species and harvest time are factors that more strongly affect the tocotrienol content in the <i>Hypericum</i> leaves than the selected drying method.
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spelling doaj-art-cd6bc2978226433aa90e74c7911e6f032025-08-20T03:03:27ZengMDPI AGPlants2223-77472025-04-01147107910.3390/plants14071079Tocopherol and Tocotrienol Content in the Leaves of the Genus <i>Hypericum</i>: Impact of Species and Drying TechniqueIeva Miķelsone0Elise Sipeniece1Dalija Segliņa2Paweł Górnaś3Institute of Horticulture, Graudu 1, LV-3701 Dobele, LatviaInstitute of Horticulture, Graudu 1, LV-3701 Dobele, LatviaInstitute of Horticulture, Graudu 1, LV-3701 Dobele, LatviaInstitute of Horticulture, Graudu 1, LV-3701 Dobele, Latviaα-Tocopherol (α-T) predominates in photosynthetic tissues, while tocotrienols (T3s) are reported very rarely. The genus <i>Hypericum</i> stands out as one of the few exceptions. Given the potential health benefits associated with tocotrienols, sourcing them from natural origins is of interest. The proper selection of plant material and the drying conditions are crucial steps in this process. Therefore, in the present study, we investigated the effects of four different drying techniques (freeze-drying, microwave–vacuum-, infrared oven and air-drying) on the tocochromanol content in leaves of three <i>Hypericum</i> species: <i>H. androsaemum</i>, <i>H. pseudohenryi</i>, and <i>H. hookerianum</i> and one hybrid <i>H. × inodorum</i>. The total tocochromanol content in the freeze-dried leaves harvested in September was 68.1–150.6 mg/100 g dry weight. α-T constituted 66.7–85.9% (<i>w</i>/<i>w</i>), while tocotrienols constituted 13–32% (<i>w</i>/<i>w</i>). <i>H. pseudohenryi</i> was characterized by the lowest tocotrienol content, while <i>H. androsaemum</i> and <i>H. hookerianum</i> had the highest, with δ-T3 and γ-T3, respectively, being predominant. Tocotrienols were more stable during drying than α-T. The greatest decrease in α-T content was observed during air-drying in the presence of sunlight, with a 27% difference compared to the absence of sunlight. The species and harvest time are factors that more strongly affect the tocotrienol content in the <i>Hypericum</i> leaves than the selected drying method.https://www.mdpi.com/2223-7747/14/7/1079St. John’s wort (<i>Hypericum perforatum</i> L.)photosyntheticherbornamental plantlipophilic bioactive compoundvitamin E
spellingShingle Ieva Miķelsone
Elise Sipeniece
Dalija Segliņa
Paweł Górnaś
Tocopherol and Tocotrienol Content in the Leaves of the Genus <i>Hypericum</i>: Impact of Species and Drying Technique
Plants
St. John’s wort (<i>Hypericum perforatum</i> L.)
photosynthetic
herb
ornamental plant
lipophilic bioactive compound
vitamin E
title Tocopherol and Tocotrienol Content in the Leaves of the Genus <i>Hypericum</i>: Impact of Species and Drying Technique
title_full Tocopherol and Tocotrienol Content in the Leaves of the Genus <i>Hypericum</i>: Impact of Species and Drying Technique
title_fullStr Tocopherol and Tocotrienol Content in the Leaves of the Genus <i>Hypericum</i>: Impact of Species and Drying Technique
title_full_unstemmed Tocopherol and Tocotrienol Content in the Leaves of the Genus <i>Hypericum</i>: Impact of Species and Drying Technique
title_short Tocopherol and Tocotrienol Content in the Leaves of the Genus <i>Hypericum</i>: Impact of Species and Drying Technique
title_sort tocopherol and tocotrienol content in the leaves of the genus i hypericum i impact of species and drying technique
topic St. John’s wort (<i>Hypericum perforatum</i> L.)
photosynthetic
herb
ornamental plant
lipophilic bioactive compound
vitamin E
url https://www.mdpi.com/2223-7747/14/7/1079
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AT elisesipeniece tocopherolandtocotrienolcontentintheleavesofthegenusihypericumiimpactofspeciesanddryingtechnique
AT dalijaseglina tocopherolandtocotrienolcontentintheleavesofthegenusihypericumiimpactofspeciesanddryingtechnique
AT pawełgornas tocopherolandtocotrienolcontentintheleavesofthegenusihypericumiimpactofspeciesanddryingtechnique