Estimation of the effect of lithium salts on cytokine production by blood cells in in vitro experiments
Aim. To study the effects of lithium salts on production of cytokines by immunocompetent cells in the whole-blood culture of patients with alcohol dependence and affective disorders.Materials and methods. The study materials were blood samples from 25 patients with alcohol dependence (AD) and 12 pat...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siberian State Medical University (Tomsk)
2021-10-01
|
| Series: | Бюллетень сибирской медицины |
| Subjects: | |
| Online Access: | https://bulletin.ssmu.ru/jour/article/view/4475 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849400293666586624 |
|---|---|
| author | T. R. Vetlugina E. V. Epimakhova D. N. Savochkina E. V. Plotnikov A. S. Boiko S. A. Ivanova N. A. Bokhan |
| author_facet | T. R. Vetlugina E. V. Epimakhova D. N. Savochkina E. V. Plotnikov A. S. Boiko S. A. Ivanova N. A. Bokhan |
| author_sort | T. R. Vetlugina |
| collection | DOAJ |
| description | Aim. To study the effects of lithium salts on production of cytokines by immunocompetent cells in the whole-blood culture of patients with alcohol dependence and affective disorders.Materials and methods. The study materials were blood samples from 25 patients with alcohol dependence (AD) and 12 patients with bipolar disorder (BD). Blood diluted 1:1 with complete RPMI-1640 medium (Gibco, UK) was added to the wells of the culture plate, then new lithium salts (succinate, fumarate, pyruvate, ascorbate) and a reference salt – lithium carbonate at a final concentration of 1.2 mmol / l per lithium ion – were added. In parallel, control samples without lithium salts were tested; the samples were incubated for a day. The concentration of cytokines (interferon (IFN) γ, interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-17A, tumor necrosis factor (TNF) α) was determined in the culture supernatants on the MAGPIX multiplex analyzer (Luminex, USA) (Center for Collective Use “Medical Genomics”, Tomsk NRMC) using the Human Cytokine / Chemokine Magnetic Bead Panel (Merck, Germany).Results. All lithium salts had a unidirectional effect on the production of cytokines by immunocompetent cells (ICC), except for lithium ascorbate and IL-8. The concentrations of cytokines in the supernatants of loaded and control samples (spontaneous production) were comparable, which indicates an absence of stimulating or suppressing effects of salts on the functional activity of ICC under the experimental conditions. The effect of lithium ascorbate as an IL-8 inducer was detected: the production of IL-8 induced by lithium ascorbate was 2.3–2.5 times higher than its spontaneous production.Conclusion. The obtained results, as well as the previously revealed antioxidant and cytoprotective properties of new lithium salts, confirmed that they are promising for development of pharmacological agents with combined action. |
| format | Article |
| id | doaj-art-cd656620872f421da0b2a71e7a425424 |
| institution | Kabale University |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2021-10-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-cd656620872f421da0b2a71e7a4254242025-08-20T03:38:06ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842021-10-01203212810.20538/1682-0363-2021-3-21-282807Estimation of the effect of lithium salts on cytokine production by blood cells in in vitro experimentsT. R. Vetlugina0E. V. Epimakhova1D. N. Savochkina2E. V. Plotnikov3A. S. Boiko4S. A. Ivanova5N. A. Bokhan6Mental Health Research Institute, Tomsk National Research Medical Center (NRMC), Russian Academy of SciencesMental Health Research Institute, Tomsk National Research Medical Center (NRMC), Russian Academy of SciencesMental Health Research Institute, Tomsk National Research Medical Center (NRMC), Russian Academy of SciencesMental Health Research Institute, Tomsk National Research Medical Center (NRMC), Russian Academy of Sciences; National Research Tomsk Polytechnic UniversityMental Health Research Institute, Tomsk National Research Medical Center (NRMC), Russian Academy of SciencesMental Health Research Institute, Tomsk National Research Medical Center (NRMC), Russian Academy of SciencesMental Health Research Institute, Tomsk National Research Medical Center (NRMC), Russian Academy of SciencesAim. To study the effects of lithium salts on production of cytokines by immunocompetent cells in the whole-blood culture of patients with alcohol dependence and affective disorders.Materials and methods. The study materials were blood samples from 25 patients with alcohol dependence (AD) and 12 patients with bipolar disorder (BD). Blood diluted 1:1 with complete RPMI-1640 medium (Gibco, UK) was added to the wells of the culture plate, then new lithium salts (succinate, fumarate, pyruvate, ascorbate) and a reference salt – lithium carbonate at a final concentration of 1.2 mmol / l per lithium ion – were added. In parallel, control samples without lithium salts were tested; the samples were incubated for a day. The concentration of cytokines (interferon (IFN) γ, interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-17A, tumor necrosis factor (TNF) α) was determined in the culture supernatants on the MAGPIX multiplex analyzer (Luminex, USA) (Center for Collective Use “Medical Genomics”, Tomsk NRMC) using the Human Cytokine / Chemokine Magnetic Bead Panel (Merck, Germany).Results. All lithium salts had a unidirectional effect on the production of cytokines by immunocompetent cells (ICC), except for lithium ascorbate and IL-8. The concentrations of cytokines in the supernatants of loaded and control samples (spontaneous production) were comparable, which indicates an absence of stimulating or suppressing effects of salts on the functional activity of ICC under the experimental conditions. The effect of lithium ascorbate as an IL-8 inducer was detected: the production of IL-8 induced by lithium ascorbate was 2.3–2.5 times higher than its spontaneous production.Conclusion. The obtained results, as well as the previously revealed antioxidant and cytoprotective properties of new lithium salts, confirmed that they are promising for development of pharmacological agents with combined action.https://bulletin.ssmu.ru/jour/article/view/4475lithium saltscytokinesblood cellsalcoholismbipolar disorder |
| spellingShingle | T. R. Vetlugina E. V. Epimakhova D. N. Savochkina E. V. Plotnikov A. S. Boiko S. A. Ivanova N. A. Bokhan Estimation of the effect of lithium salts on cytokine production by blood cells in in vitro experiments Бюллетень сибирской медицины lithium salts cytokines blood cells alcoholism bipolar disorder |
| title | Estimation of the effect of lithium salts on cytokine production by blood cells in in vitro experiments |
| title_full | Estimation of the effect of lithium salts on cytokine production by blood cells in in vitro experiments |
| title_fullStr | Estimation of the effect of lithium salts on cytokine production by blood cells in in vitro experiments |
| title_full_unstemmed | Estimation of the effect of lithium salts on cytokine production by blood cells in in vitro experiments |
| title_short | Estimation of the effect of lithium salts on cytokine production by blood cells in in vitro experiments |
| title_sort | estimation of the effect of lithium salts on cytokine production by blood cells in in vitro experiments |
| topic | lithium salts cytokines blood cells alcoholism bipolar disorder |
| url | https://bulletin.ssmu.ru/jour/article/view/4475 |
| work_keys_str_mv | AT trvetlugina estimationoftheeffectoflithiumsaltsoncytokineproductionbybloodcellsininvitroexperiments AT evepimakhova estimationoftheeffectoflithiumsaltsoncytokineproductionbybloodcellsininvitroexperiments AT dnsavochkina estimationoftheeffectoflithiumsaltsoncytokineproductionbybloodcellsininvitroexperiments AT evplotnikov estimationoftheeffectoflithiumsaltsoncytokineproductionbybloodcellsininvitroexperiments AT asboiko estimationoftheeffectoflithiumsaltsoncytokineproductionbybloodcellsininvitroexperiments AT saivanova estimationoftheeffectoflithiumsaltsoncytokineproductionbybloodcellsininvitroexperiments AT nabokhan estimationoftheeffectoflithiumsaltsoncytokineproductionbybloodcellsininvitroexperiments |