Immunomodulatory potential of Toxoplasma gondii lysate in C57BL/6 mouse model of autoimmune encephalomyelitis
Abstract Multiple sclerosis (MS) is a debilitating autoimmune disease characterized by chronic inflammation and demyelination within the central nervous system. Immunotherapy is an essential part of managing MS symptoms and progression. This study aimed to evaluate the immunomodulatory effects of To...
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SpringerOpen
2025-06-01
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| Online Access: | https://doi.org/10.1186/s13568-025-01900-x |
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| author | Maryam Hajizadeh Seyedmousa Motavallihaghi Elham Badakhsh Tohid Kazemi Abdol Sattar Pagheh Kareem Hatam-Nahavandi Ehsan Ahmadpour |
| author_facet | Maryam Hajizadeh Seyedmousa Motavallihaghi Elham Badakhsh Tohid Kazemi Abdol Sattar Pagheh Kareem Hatam-Nahavandi Ehsan Ahmadpour |
| author_sort | Maryam Hajizadeh |
| collection | DOAJ |
| description | Abstract Multiple sclerosis (MS) is a debilitating autoimmune disease characterized by chronic inflammation and demyelination within the central nervous system. Immunotherapy is an essential part of managing MS symptoms and progression. This study aimed to evaluate the immunomodulatory effects of Toxoplasma gondii lysate antigens (TLA) in a C57BL/6 mouse model of experimental autoimmune encephalomyelitis (EAE). Eighteen mice were randomly assigned into three groups: healthy controls, EAE-induced mice, and EAE-induced mice treated with TLA. Clinical scores were recorded daily for all mice. To assess immune responses, mRNA and protein expression levels in isolated splenocytes were measured using quantitative real-time PCR and ELISA, respectively. In addition, histological analyses were performed to evaluate inflammation and demyelination across the experimental groups. TLA-treated mice exhibited significantly reduced clinical scores and demyelination compared to the untreated EAE group. The treatment also decreased inflammatory cell infiltration in CNS tissues. At the molecular level, TLA modulated cytokine expression by downregulating pro-inflammatory markers (IFN-γ, IL-17, RORγT) and upregulating anti-inflammatory and regulatory markers (IL-4, GATA3, FOXP3, TGF-β, TNF-α). TLA treatment demonstrated immunomodulatory and neuroprotective effects in EAE mice, suggesting its potential as a prophylactic or therapeutic agent in MS. These findings support further exploration of parasitic antigens in the context of autoimmune neuroinflammatory diseases. |
| format | Article |
| id | doaj-art-cd61409d52db47b9a5b41a367e562b3f |
| institution | DOAJ |
| issn | 2191-0855 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | SpringerOpen |
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| series | AMB Express |
| spelling | doaj-art-cd61409d52db47b9a5b41a367e562b3f2025-08-20T03:21:06ZengSpringerOpenAMB Express2191-08552025-06-0115111010.1186/s13568-025-01900-xImmunomodulatory potential of Toxoplasma gondii lysate in C57BL/6 mouse model of autoimmune encephalomyelitisMaryam Hajizadeh0Seyedmousa Motavallihaghi1Elham Badakhsh2Tohid Kazemi3Abdol Sattar Pagheh4Kareem Hatam-Nahavandi5Ehsan Ahmadpour6Infectious and Tropical Diseases Research Center, Tabriz University of Medical SciencesDepartment of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical SciencesInfectious and Tropical Diseases Research Center, Tabriz University of Medical SciencesImmunology Research Center, Tabriz University of Medical SciencesInfectious Diseases Research Center, Birjand University of Medical SciencesIranshahr University of Medical SciencesInfectious and Tropical Diseases Research Center, Tabriz University of Medical SciencesAbstract Multiple sclerosis (MS) is a debilitating autoimmune disease characterized by chronic inflammation and demyelination within the central nervous system. Immunotherapy is an essential part of managing MS symptoms and progression. This study aimed to evaluate the immunomodulatory effects of Toxoplasma gondii lysate antigens (TLA) in a C57BL/6 mouse model of experimental autoimmune encephalomyelitis (EAE). Eighteen mice were randomly assigned into three groups: healthy controls, EAE-induced mice, and EAE-induced mice treated with TLA. Clinical scores were recorded daily for all mice. To assess immune responses, mRNA and protein expression levels in isolated splenocytes were measured using quantitative real-time PCR and ELISA, respectively. In addition, histological analyses were performed to evaluate inflammation and demyelination across the experimental groups. TLA-treated mice exhibited significantly reduced clinical scores and demyelination compared to the untreated EAE group. The treatment also decreased inflammatory cell infiltration in CNS tissues. At the molecular level, TLA modulated cytokine expression by downregulating pro-inflammatory markers (IFN-γ, IL-17, RORγT) and upregulating anti-inflammatory and regulatory markers (IL-4, GATA3, FOXP3, TGF-β, TNF-α). TLA treatment demonstrated immunomodulatory and neuroprotective effects in EAE mice, suggesting its potential as a prophylactic or therapeutic agent in MS. These findings support further exploration of parasitic antigens in the context of autoimmune neuroinflammatory diseases.https://doi.org/10.1186/s13568-025-01900-xMultiple sclerosisToxoplasmosisImmunomodulatoryAutoimmuneInflammation |
| spellingShingle | Maryam Hajizadeh Seyedmousa Motavallihaghi Elham Badakhsh Tohid Kazemi Abdol Sattar Pagheh Kareem Hatam-Nahavandi Ehsan Ahmadpour Immunomodulatory potential of Toxoplasma gondii lysate in C57BL/6 mouse model of autoimmune encephalomyelitis AMB Express Multiple sclerosis Toxoplasmosis Immunomodulatory Autoimmune Inflammation |
| title | Immunomodulatory potential of Toxoplasma gondii lysate in C57BL/6 mouse model of autoimmune encephalomyelitis |
| title_full | Immunomodulatory potential of Toxoplasma gondii lysate in C57BL/6 mouse model of autoimmune encephalomyelitis |
| title_fullStr | Immunomodulatory potential of Toxoplasma gondii lysate in C57BL/6 mouse model of autoimmune encephalomyelitis |
| title_full_unstemmed | Immunomodulatory potential of Toxoplasma gondii lysate in C57BL/6 mouse model of autoimmune encephalomyelitis |
| title_short | Immunomodulatory potential of Toxoplasma gondii lysate in C57BL/6 mouse model of autoimmune encephalomyelitis |
| title_sort | immunomodulatory potential of toxoplasma gondii lysate in c57bl 6 mouse model of autoimmune encephalomyelitis |
| topic | Multiple sclerosis Toxoplasmosis Immunomodulatory Autoimmune Inflammation |
| url | https://doi.org/10.1186/s13568-025-01900-x |
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