The effect of inhibition of leukotriene synthesis on the activity of interleukin-8 and granulocyte-macrophage colony-stimulating factor
The cytokines interleukin-8 (IL-8) and granulocyte-macrophage colony-stimulating factor (GM-CSF) enhanced the extracellular release of arachidonate metabolites from ionophore-stimulated neutrophils by 145 ± 10% (mean ± S.E.M., n = 13) and 182 ± 11% (n = 16), respectively. To determine whether enhanc...
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| Format: | Article |
| Language: | English |
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Wiley
1993-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935193000286 |
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| author | P. J. Roberts A. R. Pizzey D. C. Linch |
| author_facet | P. J. Roberts A. R. Pizzey D. C. Linch |
| author_sort | P. J. Roberts |
| collection | DOAJ |
| description | The cytokines interleukin-8 (IL-8) and granulocyte-macrophage colony-stimulating factor (GM-CSF) enhanced the extracellular release of arachidonate metabolites from ionophore-stimulated neutrophils by 145 ± 10% (mean ± S.E.M., n = 13) and 182 ± 11% (n = 16), respectively. To determine whether enhanced leukotriene production mediates the effects of these cytokines on neutrophil activity, two different specific arachidonate 5-lipoxygenase (5-LO) inhibitors, piriprost and MK-886, were used to inhibit leukotriene synthesis. Neither inhibitor affected the upregulation of CD11b β2-integrin expression or priming of superoxide generation stimulated by IL-8 and GM-CSF. It is concluded that leukotrienes do not mediate either the direct or priming effects of these cytokines and that these classes of anti-inflammatory drugs are therefore unlikely to inhibit the effects of IL-8 and GM-CSF on neutrophil activation. |
| format | Article |
| id | doaj-art-cd5c51f5b4e74997a9df1dd1086bfe48 |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1993-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-cd5c51f5b4e74997a9df1dd1086bfe482025-08-20T03:19:35ZengWileyMediators of Inflammation0962-93511466-18611993-01-012321121610.1155/S0962935193000286The effect of inhibition of leukotriene synthesis on the activity of interleukin-8 and granulocyte-macrophage colony-stimulating factorP. J. Roberts0A. R. Pizzey1D. C. Linch2Department of Haematology, University College London Medical School, 98 Chenies Mews, London WC1E 6HX, UKDepartment of Haematology, University College London Medical School, 98 Chenies Mews, London WC1E 6HX, UKDepartment of Haematology, University College London Medical School, 98 Chenies Mews, London WC1E 6HX, UKThe cytokines interleukin-8 (IL-8) and granulocyte-macrophage colony-stimulating factor (GM-CSF) enhanced the extracellular release of arachidonate metabolites from ionophore-stimulated neutrophils by 145 ± 10% (mean ± S.E.M., n = 13) and 182 ± 11% (n = 16), respectively. To determine whether enhanced leukotriene production mediates the effects of these cytokines on neutrophil activity, two different specific arachidonate 5-lipoxygenase (5-LO) inhibitors, piriprost and MK-886, were used to inhibit leukotriene synthesis. Neither inhibitor affected the upregulation of CD11b β2-integrin expression or priming of superoxide generation stimulated by IL-8 and GM-CSF. It is concluded that leukotrienes do not mediate either the direct or priming effects of these cytokines and that these classes of anti-inflammatory drugs are therefore unlikely to inhibit the effects of IL-8 and GM-CSF on neutrophil activation.http://dx.doi.org/10.1155/S0962935193000286 |
| spellingShingle | P. J. Roberts A. R. Pizzey D. C. Linch The effect of inhibition of leukotriene synthesis on the activity of interleukin-8 and granulocyte-macrophage colony-stimulating factor Mediators of Inflammation |
| title | The effect of inhibition of leukotriene synthesis on the activity of interleukin-8 and granulocyte-macrophage colony-stimulating factor |
| title_full | The effect of inhibition of leukotriene synthesis on the activity of interleukin-8 and granulocyte-macrophage colony-stimulating factor |
| title_fullStr | The effect of inhibition of leukotriene synthesis on the activity of interleukin-8 and granulocyte-macrophage colony-stimulating factor |
| title_full_unstemmed | The effect of inhibition of leukotriene synthesis on the activity of interleukin-8 and granulocyte-macrophage colony-stimulating factor |
| title_short | The effect of inhibition of leukotriene synthesis on the activity of interleukin-8 and granulocyte-macrophage colony-stimulating factor |
| title_sort | effect of inhibition of leukotriene synthesis on the activity of interleukin 8 and granulocyte macrophage colony stimulating factor |
| url | http://dx.doi.org/10.1155/S0962935193000286 |
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