The Shaker Potassium Channel Is No Target for Xenon Anesthesia in Short-Sleeping Drosophila melanogaster Mutants
Background. Xenon seems to be an ideal anesthetic drug. To explore if next to the antagonism at the NMDA-receptor other molecular targets are involved, we tested the xenon requirement in short sleeping Drosophila shaker mutants and in 𝑛𝑎[ℎ𝑎𝑟38]. Methods. The Drosophila melanogaster strains wildtype...
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/373709 |
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| author | C. Schaper J. Höcker R. Böhm T. Roeder B. Bein |
| author_facet | C. Schaper J. Höcker R. Böhm T. Roeder B. Bein |
| author_sort | C. Schaper |
| collection | DOAJ |
| description | Background. Xenon seems to be an ideal anesthetic drug. To explore if next to the antagonism at the NMDA-receptor other molecular targets are involved, we tested the xenon requirement in short sleeping Drosophila shaker mutants and in 𝑛𝑎[ℎ𝑎𝑟38]. Methods. The Drosophila melanogaster strains wildtype Canton-S, 𝑛𝑎[ℎ𝑎𝑟38], 𝑠ℎ102 and 𝑠ℎ𝑚𝑛𝑠, were raised and sleep was measured. Based on the response of the flies at different xenon concentrations, logEC50 values were calculated. Results. The logEC50-values for WT Canton-S were 1.671 (1.601–1.742 95%-confidence intervall; 𝑛=238; P versus 𝑠ℎ102 > 0,05), for 𝑠ℎ𝑚𝑛𝑠 1.711 (1.650–1.773; 𝑛=242; P versus WT Canton-S > 0,05). The logEC50-value for 𝑠ℎ102 was 1.594 (1.493–1.694; 𝑛=261; P versus 𝑠ℎ𝑚𝑛𝑠 > 0.05). The logEC-value of 𝑛𝑎[ℎ𝑎𝑟38] was 2.076 (1.619–2.532; 𝑛=207; P versus 𝑠ℎ𝑚𝑛𝑠 < 0.05, versus 𝑠ℎ102 < 0.05, versus WT Canton-S < 0.05). P values for all shaker mutants were 𝑃>0.05, while 𝑛𝑎[ℎ𝑎𝑟38] was found to be hyposensitive compared to wildtype (P < 0.05). Conclusions. The xenon requirement in Drosophila melanogaster is not influenced by a single gene mutation at the shaker locus, whereas a reduced expression of a nonselective cation channel leads to an increased xenon requirement. This supports the thesis that xenon mediates its effects not only via an antagonism at the NMDA-receptor. |
| format | Article |
| id | doaj-art-cd5444907e3147acb7ac2f0c386b2e23 |
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| publishDate | 2012-01-01 |
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| spelling | doaj-art-cd5444907e3147acb7ac2f0c386b2e232025-08-20T02:19:51ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/373709373709The Shaker Potassium Channel Is No Target for Xenon Anesthesia in Short-Sleeping Drosophila melanogaster MutantsC. Schaper0J. Höcker1R. Böhm2T. Roeder3B. Bein4Department of Anaesthesiology and Operrative Intensive Care Medicine, University Hospital Schleswig-Holstein, Campus Kiel, Schwanenweg 21, 24105 Kiel, GermanyDepartment of Anaesthesiology and Operrative Intensive Care Medicine, University Hospital Schleswig-Holstein, Campus Kiel, Schwanenweg 21, 24105 Kiel, GermanyInstitute of Clinical and Experimental Pharmacology, University Hospital Schleswig-Holstein, Campus Kiel, Arnold-Heller-Straße 3, Haus 30, 24105 Kiel, GermanyDepartment of Zoophysiology, CAU Kiel, Olshausenstraße 40, 24098 Kiel, GermanyDepartment of Anaesthesiology and Operrative Intensive Care Medicine, University Hospital Schleswig-Holstein, Campus Kiel, Schwanenweg 21, 24105 Kiel, GermanyBackground. Xenon seems to be an ideal anesthetic drug. To explore if next to the antagonism at the NMDA-receptor other molecular targets are involved, we tested the xenon requirement in short sleeping Drosophila shaker mutants and in 𝑛𝑎[ℎ𝑎𝑟38]. Methods. The Drosophila melanogaster strains wildtype Canton-S, 𝑛𝑎[ℎ𝑎𝑟38], 𝑠ℎ102 and 𝑠ℎ𝑚𝑛𝑠, were raised and sleep was measured. Based on the response of the flies at different xenon concentrations, logEC50 values were calculated. Results. The logEC50-values for WT Canton-S were 1.671 (1.601–1.742 95%-confidence intervall; 𝑛=238; P versus 𝑠ℎ102 > 0,05), for 𝑠ℎ𝑚𝑛𝑠 1.711 (1.650–1.773; 𝑛=242; P versus WT Canton-S > 0,05). The logEC50-value for 𝑠ℎ102 was 1.594 (1.493–1.694; 𝑛=261; P versus 𝑠ℎ𝑚𝑛𝑠 > 0.05). The logEC-value of 𝑛𝑎[ℎ𝑎𝑟38] was 2.076 (1.619–2.532; 𝑛=207; P versus 𝑠ℎ𝑚𝑛𝑠 < 0.05, versus 𝑠ℎ102 < 0.05, versus WT Canton-S < 0.05). P values for all shaker mutants were 𝑃>0.05, while 𝑛𝑎[ℎ𝑎𝑟38] was found to be hyposensitive compared to wildtype (P < 0.05). Conclusions. The xenon requirement in Drosophila melanogaster is not influenced by a single gene mutation at the shaker locus, whereas a reduced expression of a nonselective cation channel leads to an increased xenon requirement. This supports the thesis that xenon mediates its effects not only via an antagonism at the NMDA-receptor.http://dx.doi.org/10.1100/2012/373709 |
| spellingShingle | C. Schaper J. Höcker R. Böhm T. Roeder B. Bein The Shaker Potassium Channel Is No Target for Xenon Anesthesia in Short-Sleeping Drosophila melanogaster Mutants The Scientific World Journal |
| title | The Shaker Potassium Channel Is No Target for Xenon Anesthesia in Short-Sleeping Drosophila melanogaster Mutants |
| title_full | The Shaker Potassium Channel Is No Target for Xenon Anesthesia in Short-Sleeping Drosophila melanogaster Mutants |
| title_fullStr | The Shaker Potassium Channel Is No Target for Xenon Anesthesia in Short-Sleeping Drosophila melanogaster Mutants |
| title_full_unstemmed | The Shaker Potassium Channel Is No Target for Xenon Anesthesia in Short-Sleeping Drosophila melanogaster Mutants |
| title_short | The Shaker Potassium Channel Is No Target for Xenon Anesthesia in Short-Sleeping Drosophila melanogaster Mutants |
| title_sort | shaker potassium channel is no target for xenon anesthesia in short sleeping drosophila melanogaster mutants |
| url | http://dx.doi.org/10.1100/2012/373709 |
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