CLINICAL AND DIAGNOSTIC VALUE OF YKL-40 AND NGAL IN PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE
Introduction. Chronic obstructive pulmonary disease (COPD) is one of the most common diseases of the bronchopulmonary system. Determination of new inflammatory markers for early diagnosis, optimization and monitoring of therapy is a promising direction of modern research. Objective. To evaluate the...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2019-08-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/3174 |
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| Summary: | Introduction. Chronic obstructive pulmonary disease (COPD) is one of the most common diseases of the bronchopulmonary system. Determination of new inflammatory markers for early diagnosis, optimization and monitoring of therapy is a promising direction of modern research. Objective. To evaluate the levels of YKL-40 and NGAL in serum and induced sputum and to determine their significance as markers of inflammation in patients with COPD. Materials and Methods. The study included 50 patients with COPD, 60 patients with asthma. The control group consisted of 30 volunteers, comparable in age and sex, without allergic and bronchoobstructive diseases. Clinical and anamnestic data, indices of external respiratory function were evaluated. Induced sputum was collected with subsequent assessment of cellular composition. Immunophenotyping of lymphocytes by flow cytometry was performed. The concentration of YKL-40 and NGAL in serum was determined using an enzyme immunoassay test system (R&D Systems). The obtained data were processed using STATISTICA and SPSS Statistics software systems. Results. A significant increase in the absolute number of T-lymphocytes in patients of the studied groups compared to the control group was found. The highest number of T-cytotoxic lymphocytes and NK-cells was registered in the COPD group. Analysis of the cellular composition of induced sputum in patients with COPD revealed the predominance of neutrophilic pattern of inflammation. The maximum concentration of YKL-40 in serum was registered in COPD patients. The level of NGAL in serum of COPD patients was not significantly different from that of the asthma group. NGAL level in induced sputum was significantly higher in COPD group. Correlation analysis confirmed the correlation of YKL-40 and NGAL levels in serum with neutrophilic inflammation indices, smoking index, number of hospitalizations due to COPD exacerbation during a calendar year. It was found that YKL-40 and NGAL levels were significantly increased in patients with frequent COPD exacerbations compared to the group with infrequent exacerbations. Conclusions. YKL-40 and NGAL are promising markers of neutrophilic airway inflammation in COPD patients. The obtained data allow us to consider YKL-40 and NGAL as markers of non-T2-endotype COPD with neutrophilic inflammation pattern and high risk of exacerbations. |
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| ISSN: | 1563-0625 2313-741X |