Analysis of Nabumetone in Bulk and Tablet Formulation by a New and Validated Reverse Phase High Performance Liquid Chromatography
RP-HPLC analytical method for the estimation of nabumetone in pharmaceutical dosage forms was developed and validated. A Hypersil ODS C18, 4.6 mm x 250 mm, 5 μm column from Supelco (India), with mobile phase comprised of acetonitrile: triple distilled water (50:50) with a total run time of 18 min wa...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2009/795896 |
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| author | Prafulla Kumar Sahu M. Mathrusri Annapurna |
| author_facet | Prafulla Kumar Sahu M. Mathrusri Annapurna |
| author_sort | Prafulla Kumar Sahu |
| collection | DOAJ |
| description | RP-HPLC analytical method for the estimation of nabumetone in pharmaceutical dosage forms was developed and validated. A Hypersil ODS C18, 4.6 mm x 250 mm, 5 μm column from Supelco (India), with mobile phase comprised of acetonitrile: triple distilled water (50:50) with a total run time of 18 min was used and the wavelength of the detector was set at 230 nm. Stavudin is used as internal standard. The retention times were 14.167 min and 1.967 min for nabumetone and stavudin (IS) respectively. The extraction recovery of nabumetone from pharmaceutical dosage form (tablets) was >101% and the calibration curve was linear (r2 = 0.995) over nabumetone concentrations ranging from 1 to 200 µg/mL. The method had an accuracy of >99% and LOD and LOQ of 0.17482 µg/mL and 0.5827 µg/mL respectively. The method reported is simple, reliable, precise and accurate and has the capability of being used for determination of nabumetone in bulk and pharmaceutical dosage forms. |
| format | Article |
| id | doaj-art-cd477f79127149f5a829ff294b1803e0 |
| institution | OA Journals |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
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| series | E-Journal of Chemistry |
| spelling | doaj-art-cd477f79127149f5a829ff294b1803e02025-08-20T02:19:52ZengWileyE-Journal of Chemistry0973-49452090-98102009-01-016S1S59S6410.1155/2009/795896Analysis of Nabumetone in Bulk and Tablet Formulation by a New and Validated Reverse Phase High Performance Liquid ChromatographyPrafulla Kumar Sahu0M. Mathrusri Annapurna1Gayatri Institute of Pharmaceutical Science and Technology, Gunupur, Rayagada, Orissa, IndiaDepartment of Pharmaceutical Analysis & Quality Assurance, Roland Institute of Pharmaceutical Sciences, Berhampur, Orissa, IndiaRP-HPLC analytical method for the estimation of nabumetone in pharmaceutical dosage forms was developed and validated. A Hypersil ODS C18, 4.6 mm x 250 mm, 5 μm column from Supelco (India), with mobile phase comprised of acetonitrile: triple distilled water (50:50) with a total run time of 18 min was used and the wavelength of the detector was set at 230 nm. Stavudin is used as internal standard. The retention times were 14.167 min and 1.967 min for nabumetone and stavudin (IS) respectively. The extraction recovery of nabumetone from pharmaceutical dosage form (tablets) was >101% and the calibration curve was linear (r2 = 0.995) over nabumetone concentrations ranging from 1 to 200 µg/mL. The method had an accuracy of >99% and LOD and LOQ of 0.17482 µg/mL and 0.5827 µg/mL respectively. The method reported is simple, reliable, precise and accurate and has the capability of being used for determination of nabumetone in bulk and pharmaceutical dosage forms.http://dx.doi.org/10.1155/2009/795896 |
| spellingShingle | Prafulla Kumar Sahu M. Mathrusri Annapurna Analysis of Nabumetone in Bulk and Tablet Formulation by a New and Validated Reverse Phase High Performance Liquid Chromatography E-Journal of Chemistry |
| title | Analysis of Nabumetone in Bulk and Tablet Formulation by a New and Validated Reverse Phase High Performance Liquid Chromatography |
| title_full | Analysis of Nabumetone in Bulk and Tablet Formulation by a New and Validated Reverse Phase High Performance Liquid Chromatography |
| title_fullStr | Analysis of Nabumetone in Bulk and Tablet Formulation by a New and Validated Reverse Phase High Performance Liquid Chromatography |
| title_full_unstemmed | Analysis of Nabumetone in Bulk and Tablet Formulation by a New and Validated Reverse Phase High Performance Liquid Chromatography |
| title_short | Analysis of Nabumetone in Bulk and Tablet Formulation by a New and Validated Reverse Phase High Performance Liquid Chromatography |
| title_sort | analysis of nabumetone in bulk and tablet formulation by a new and validated reverse phase high performance liquid chromatography |
| url | http://dx.doi.org/10.1155/2009/795896 |
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